FastQCFastQC Report
Fri 27 May 2016
SRR940070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753596
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42110.5587874670247719No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31030.41175908577009435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19830.2631383393754744No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.12367369253552302No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17000.075.0289461
GTATCAA33850.060.042631
ATCAACG42300.047.334343
TCAACGC42850.047.0049134
TATCAAC43150.046.8162162
CAACGCA43450.046.1634525
AACGCAG44700.044.866576
GTGGTAT6550.044.588081
ACGCAGA51850.038.4398167
CGCAGAG53400.037.1012238
TGGTATC7800.034.3791662
GTACATG36700.034.1049351
GCAGAGT57900.033.9094439
TATCACG902.3709492E-433.105862
TACATGG37950.032.9750062
ACATGGG38250.031.6258343
GAGTACT39800.028.70142212-13
GTACTTT40350.028.23647714-15
CAGAGTA57450.027.8060610-11
CATGGGG22250.027.3179154
Produced by FastQC (version 0.11.5)