FastQCFastQC Report
Fri 27 May 2016
SRR940069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177180
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14500.059.2553021
GTATCAA25250.051.2780041
ATCAACG30000.041.5289273
TCAACGC30650.040.844444
CAACGCA31500.039.721995
TATCAAC33450.038.3524932
AACGCAG32350.038.3099256
GTACATG69450.032.3034061
TACATGG70000.031.454272
ACGCAGA39050.031.279167
CGCAGAG40750.029.9742628
ACATGGG69700.029.7626883
CATGGGG40800.025.71564
TAAGGTA3500.025.5486164
GCAGAGT48450.024.581019
CATGGGA31850.023.9576764
GTATTGG4450.022.7940581
CGTACAC1602.5786163E-422.3540883
ATGGGGC12450.022.0145995
GAGTACA54550.021.9853861
Produced by FastQC (version 0.11.5)