##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940069_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1177180 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.661677908221343 34.0 31.0 34.0 30.0 34.0 2 31.641041302094838 34.0 31.0 34.0 30.0 34.0 3 31.857941011570023 34.0 31.0 34.0 30.0 34.0 4 34.9964465927046 37.0 37.0 37.0 33.0 37.0 5 35.13141320783568 37.0 37.0 37.0 33.0 37.0 6 35.105663534888464 37.0 37.0 37.0 33.0 37.0 7 35.095544436704664 37.0 37.0 37.0 33.0 37.0 8 35.030291883993954 37.0 37.0 37.0 33.0 37.0 9 36.65612820469257 39.0 39.0 39.0 34.0 39.0 10-11 36.6849330603646 39.0 39.0 39.0 34.0 39.0 12-13 36.6363173006677 39.0 39.0 39.0 33.5 39.0 14-15 38.16057314939091 41.0 40.0 41.0 33.5 41.0 16-17 38.220253487147254 41.0 40.0 41.0 34.0 41.0 18-19 38.256255203112524 41.0 40.0 41.0 33.5 41.0 20-21 38.29104172683871 41.0 40.0 41.0 34.0 41.0 22-23 38.33619667340594 41.0 40.0 41.0 34.0 41.0 24-25 38.404441546747314 41.0 40.0 41.0 33.5 41.0 26-27 38.45077558232386 41.0 40.0 41.0 33.5 41.0 28-29 38.412345605599825 41.0 39.5 41.0 33.0 41.0 30-31 38.357986034421245 41.0 39.0 41.0 33.0 41.0 32-33 38.31602006490087 41.0 39.0 41.0 33.0 41.0 34-35 38.23882796173908 41.0 39.0 41.0 33.0 41.0 36-37 38.10969945123091 41.0 39.0 41.0 33.0 41.0 38-39 38.01305662685401 41.0 39.0 41.0 32.0 41.0 40-41 37.92744652474558 41.0 38.5 41.0 32.0 41.0 42-43 37.770990842521954 40.5 38.0 41.0 32.0 41.0 44-45 37.61599373078034 40.0 38.0 41.0 31.0 41.0 46-47 37.45871277119897 40.0 38.0 41.0 31.0 41.0 48-49 37.311115122581086 40.0 37.5 41.0 30.5 41.0 50-51 37.144476630591754 40.0 37.0 41.0 30.0 41.0 52-53 37.27304745238621 40.0 37.0 41.0 31.0 41.0 54-55 37.071162863793134 40.0 37.0 41.0 30.0 41.0 56-57 36.95428948843847 40.0 36.0 41.0 30.0 41.0 58-59 36.75966079953788 40.0 36.0 41.0 30.5 41.0 60-61 36.51254311150376 39.0 35.0 41.0 31.0 41.0 62-63 36.22237380859342 39.0 35.0 41.0 30.0 41.0 64-65 35.904752034523185 38.5 35.0 41.0 30.0 41.0 66-67 35.58264666406157 37.5 35.0 40.5 30.0 41.0 68-69 35.23349530233269 37.0 35.0 40.0 30.0 41.0 70-71 34.8700954824241 36.5 35.0 39.0 30.0 41.0 72-73 34.50242188960057 36.0 35.0 39.0 29.5 41.0 74-75 34.12883458774359 35.5 34.5 38.5 29.0 40.5 76-77 33.76010763009905 35.0 34.0 37.0 29.0 39.0 78-79 33.42321395198695 35.0 34.0 37.0 29.0 39.0 80-81 33.1075901731256 35.0 34.0 36.5 28.0 39.0 82-83 32.80558198406361 35.0 34.0 36.0 28.0 37.0 84-85 32.57197922152942 35.0 34.0 36.0 27.5 37.0 86-87 32.36229421159041 35.0 34.0 35.0 27.0 36.5 88-89 32.1946698890569 35.0 34.0 35.0 27.0 36.0 90-91 32.04519487249189 35.0 34.0 35.0 27.0 36.0 92-93 31.899728588661038 35.0 34.0 35.0 26.5 36.0 94-95 31.7891847465978 35.0 33.0 35.0 26.0 35.5 96-97 31.687365143818276 35.0 33.0 35.0 25.0 35.0 98-99 31.613576088618565 35.0 33.0 35.0 25.0 35.0 100-101 31.517606483290578 35.0 33.0 35.0 25.0 35.0 102-103 30.812176982279688 34.0 32.0 35.0 24.5 35.0 104-105 31.71340958901782 35.0 33.5 35.0 25.5 35.0 106-107 31.818592738578637 35.0 34.0 35.0 27.0 35.0 108-109 31.799513243514163 35.0 34.0 35.0 26.0 35.0 110-111 31.746139078135883 35.0 34.0 35.0 25.0 35.0 112-113 31.690152313155167 35.0 34.0 35.0 25.0 35.0 114-115 31.619847856742382 35.0 34.0 35.0 25.0 35.0 116-117 31.539301126420767 35.0 34.0 35.0 25.0 35.0 118-119 31.45252892505819 35.0 34.0 35.0 25.0 35.0 120-121 31.357475067534278 35.0 34.0 35.0 24.5 35.0 122-123 31.215914303674886 35.0 34.0 35.0 24.0 35.0 124-125 29.975115955079087 34.5 31.5 35.0 12.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04883493454443766 1101 2 0.26283918869367184 1101 3 0.3360438733638311 1101 4 0.26645898731566575 1101 5 0.2817903522761611 1101 6 0.36955252409457984 1101 7 0.3937284238502414 1101 8 0.3680163449080638 1101 9 0.34793181236415194 1101 10-11 0.21433122509738922 1101 12-13 0.26194595476506066 1101 14-15 0.35319706092041514 1101 16-17 0.3717873837429977 1101 18-19 0.3255588722932572 1101 20-21 0.27406155078101335 1101 22-23 0.20848848599943892 1101 24-25 0.20334472484614707 1101 26-27 0.27681920416787875 1101 28-29 0.32825820915989823 1101 30-31 0.36057739692318336 1101 32-33 0.3938483670133479 1101 34-35 0.423750476258391 1101 36-37 0.3718020260133983 1101 38-39 0.38375173185170297 1101 40-41 0.4060201098311822 1101 42-43 0.40241134968365344 1101 44-45 0.40550685115204743 1101 46-47 0.5009054508806798 1101 48-49 0.5062110231277757 1101 50-51 0.4255116672340691 1101 52-53 0.4666765374921269 1101 54-55 0.4523587956108841 1101 56-57 0.4440185150859719 1101 58-59 0.37077838421459575 1101 60-61 0.40797591550146706 1101 62-63 0.3181467534524032 1101 64-65 0.3696823296898586 1101 66-67 0.28281594504515795 1101 68-69 0.3433211283080624 1101 70-71 0.40174102330252737 1101 72-73 0.3674139551712443 1101 74-75 0.4061568161204647 1101 76-77 0.4848666882104382 1101 78-79 0.4106978692778327 1101 80-81 0.3409861854538434 1101 82-83 0.4752227449473949 1101 84-85 0.40755522589387283 1101 86-87 0.37537356850064185 1101 88-89 0.29589851643856235 1101 90-91 0.22859208575914636 1101 92-93 0.32350500419597594 1101 94-95 0.3046252428680205 1101 96-97 0.3123583825677443 1101 98-99 0.26078960614408686 1101 100-101 0.19227975617448934 1101 102-103 0.25096109297591696 1101 104-105 0.2401313106582954 1101 106-107 0.2617842630623599 1101 108-109 0.3449613999130534 1101 110-111 0.23953888856593508 1101 112-113 0.35742872796423697 1101 114-115 0.35144074700222205 1101 116-117 0.43317131564690214 1101 118-119 0.46574246817734277 1101 120-121 0.32885738428567635 1101 122-123 0.28625436822398953 1101 124-125 0.24664760873152858 1102 1 0.14453535063320544 1102 2 0.2059737240612165 1102 3 0.2743240397993354 1102 4 0.3281788208801615 1102 5 0.3240927101124953 1102 6 0.3293306100862594 1102 7 0.24809735588907245 1102 8 0.3007486611355219 1102 9 0.3756710634043756 1102 10-11 0.5381869809919735 1102 12-13 0.5161068424210882 1102 14-15 0.600076395178391 1102 16-17 0.4789302408858447 1102 18-19 0.42333973221003873 1102 20-21 0.3766967796298317 1102 22-23 0.4390710102712774 1102 24-25 0.46998827547027133 1102 26-27 0.5230050571498452 1102 28-29 0.5089100815593497 1102 30-31 0.4864442485181897 1102 32-33 0.5516153573046125 1102 34-35 0.5891457605857155 1102 36-37 0.6460738706735896 1102 38-39 0.6604507609779162 1102 40-41 0.6650353664192465 1102 42-43 0.523770572984624 1102 44-45 0.4543972811104382 1102 46-47 0.5279512206448942 1102 48-49 0.6102332145286056 1102 50-51 0.5857058419913486 1102 52-53 0.545039921680754 1102 54-55 0.6895293920047791 1102 56-57 0.668012967235768 1102 58-59 0.5368671498179225 1102 60-61 0.5345362483724827 1102 62-63 0.572654381746446 1102 64-65 0.543052648691237 1102 66-67 0.5217202446290727 1102 68-69 0.537842626227615 1102 70-71 0.5147784712914287 1102 72-73 0.5036830259063336 1102 74-75 0.544853071321576 1102 76-77 0.4848666882104382 1102 78-79 0.5504343463929473 1102 80-81 0.4307920106965568 1102 82-83 0.45545852858123936 1102 84-85 0.41102263227390523 1102 86-87 0.3341114325783181 1102 88-89 0.2837625941084667 1102 90-91 0.30348806356774105 1102 92-93 0.35228447715020295 1102 94-95 0.3614907075004723 1102 96-97 0.3387106710559564 1102 98-99 0.30378544525643036 1102 100-101 0.3396445273256674 1102 102-103 0.2981178197442951 1102 104-105 0.31433380719089143 1102 106-107 0.37343474849925684 1102 108-109 0.3945453111474464 1102 110-111 0.2901630217143385 1102 112-113 0.35014717456617817 1102 114-115 0.39027569845853094 1102 116-117 0.3808134793084861 1102 118-119 0.3631072393285244 1102 120-121 0.3028518364354653 1102 122-123 0.30948599097017393 1102 124-125 0.25392916212958383 1103 1 -0.23957767468119684 1103 2 -0.15184208059173443 1103 3 -0.07551813020904419 1103 4 -0.20091005859223543 1103 5 0.02212874873837478 1103 6 -0.12761194950366672 1103 7 -0.18651659997826187 1103 8 -0.2777528592965055 1103 9 -0.20642199935614514 1103 10-11 -0.26968883825860956 1103 12-13 -0.17841465549846447 1103 14-15 -0.041151580024283874 1103 16-17 -0.16620054986947252 1103 18-19 -0.07767295579058242 1103 20-21 -0.21634311374391757 1103 22-23 -0.18609862663574006 1103 24-25 -0.08162754523634419 1103 26-27 -0.10297193009868266 1103 28-29 -0.08982139838383318 1103 30-31 -0.013714888167619677 1103 32-33 0.005151645135740068 1103 34-35 0.04126101848048336 1103 36-37 0.07698941529439196 1103 38-39 0.06481941301085214 1103 40-41 0.025598962292342264 1103 42-43 -0.021513380997340903 1103 44-45 -0.2016322725788271 1103 46-47 -0.13647150986062684 1103 48-49 -0.13188275271443217 1103 50-51 -0.14673358806597747 1103 52-53 -0.19230101163491042 1103 54-55 -0.17901670536634384 1103 56-57 -0.2715603597756626 1103 58-59 -0.3334378512740983 1103 60-61 -0.31541839067191546 1103 62-63 -0.3708091605521844 1103 64-65 -0.3057324990492063 1103 66-67 -0.26737744569560107 1103 68-69 -0.36808519198508804 1103 70-71 -0.2588129590347066 1103 72-73 -0.3017245413464167 1103 74-75 -0.30754415251326606 1103 76-77 -0.2073008934532794 1103 78-79 -0.18638717598045673 1103 80-81 -0.18855257016261362 1103 82-83 -0.09993083828809546 1103 84-85 -0.1095307340503453 1103 86-87 -0.10525235668551147 1103 88-89 -0.1268665231449333 1103 90-91 -0.10003683735683921 1103 92-93 -0.06572886233081832 1103 94-95 -0.0468568283542794 1103 96-97 -0.06838850516669481 1103 98-99 -0.05023021267342642 1103 100-101 -0.01985364610252205 1103 102-103 -0.07508570907671341 1103 104-105 -0.10174982497095897 1103 106-107 -0.08682914772682437 1103 108-109 -0.01475937820467621 1103 110-111 -0.015015407377898526 1103 112-113 -0.04977311708796961 1103 114-115 -0.059692380869005746 1103 116-117 -0.06916906699049008 1103 118-119 -0.07617721493064877 1103 120-121 -0.014812055683862013 1103 122-123 -0.08490931762432297 1103 124-125 -0.13277951786660935 1104 1 0.051808597189598515 1104 2 0.21424136489951806 1104 3 0.22730824933335114 1104 4 0.13489074894477682 1104 5 0.11297666260109906 1104 6 0.24587391859861896 1104 7 0.20042821832591073 1104 8 0.17379491997671437 1104 9 0.24781756995744786 1104 10-11 0.21180510521379858 1104 12-13 0.26539422740867025 1104 14-15 0.336708446893617 1104 16-17 0.28097307058813215 1104 18-19 0.32003902267508977 1104 20-21 0.23587761303939203 1104 22-23 0.2516537286805516 1104 24-25 0.3177668020045701 1104 26-27 0.23451124621356456 1104 28-29 0.20459313728238726 1104 30-31 0.2595018009306287 1104 32-33 0.3863764096041109 1104 34-35 0.3679770346312239 1104 36-37 0.33540125917056685 1104 38-39 0.240122239390395 1104 40-41 0.258438800784937 1104 42-43 0.12922686126376703 1104 44-45 0.27346866954768956 1104 46-47 0.2908501590491994 1104 48-49 0.1608494141765533 1104 50-51 0.18570248192056482 1104 52-53 0.14047153210668029 1104 54-55 0.07367508467403638 1104 56-57 0.0591933414356447 1104 58-59 0.07941544616869578 1104 60-61 0.1421859161921475 1104 62-63 0.16014642491214914 1104 64-65 0.11553264226976978 1104 66-67 0.10610842240668461 1104 68-69 0.13359717679014693 1104 70-71 0.18264060758709633 1104 72-73 0.19928487991872146 1104 74-75 0.13996594169937993 1104 76-77 0.18233571489126632 1104 78-79 0.19416464097782438 1104 80-81 0.1270598120715789 1104 82-83 0.27658329360961886 1104 84-85 0.15931179623228786 1104 86-87 0.12826042931718007 1104 88-89 0.15408906231939312 1104 90-91 0.19034001324882155 1104 92-93 0.16610155489663825 1104 94-95 0.07158193342230135 1104 96-97 0.14527232928821832 1104 98-99 0.2038074723871368 1104 100-101 0.09050974555293223 1104 102-103 0.015762204786007317 1104 104-105 0.07321652765353548 1104 106-107 0.01646829507263803 1104 108-109 0.04008584386800251 1104 110-111 0.08828203542156032 1104 112-113 0.13797923822831493 1104 114-115 -0.03243800671018704 1104 116-117 -0.058401906828983385 1104 118-119 -0.06776537105447034 1104 120-121 -0.011783791888436923 1104 122-123 -0.05866436473065306 1104 124-125 0.0465609935735003 1105 1 -0.08255154151508037 1105 2 -0.04031539383611005 1105 3 -0.011424667553399104 1105 4 0.006618758792704682 1105 5 -0.11038134102062003 1105 6 -0.02036478136120934 1105 7 -0.0416672092830197 1105 8 5.850895096060071E-4 1105 9 -0.05033113407302636 1105 10-11 0.05371343470955736 1105 12-13 0.08531797190061496 1105 14-15 0.093154892118541 1105 16-17 0.044963564703515146 1105 18-19 -0.09281204793941811 1105 20-21 -0.22272943460493622 1105 22-23 -0.11431993417586739 1105 24-25 -0.1058175809813946 1105 26-27 -0.08758432391237392 1105 28-29 -0.10869465889690844 1105 30-31 -0.04813274626165054 1105 32-33 -0.078440148734785 1105 34-35 0.007519893504650099 1105 36-37 0.0030769759643334282 1105 38-39 -0.06045148065096839 1105 40-41 -0.0822567980225557 1105 42-43 -0.04216476390224244 1105 44-45 -0.17072055563185273 1105 46-47 -0.3054285436540809 1105 48-49 -0.30058169710875404 1105 50-51 -0.2967011209094679 1105 52-53 -0.1751787062084844 1105 54-55 -0.12752441865418263 1105 56-57 -0.18139006976244332 1105 58-59 -0.14831805236560314 1105 60-61 -0.15339993115610184 1105 62-63 -0.058814706245364334 1105 64-65 -0.07283743521494301 1105 66-67 -0.13068247752588746 1105 68-69 -0.12127690497720778 1105 70-71 -0.1438901364496772 1105 72-73 -0.08534831247122554 1105 74-75 0.022483825357404896 1105 76-77 -0.005103993732703316 1105 78-79 -0.11157843826252645 1105 80-81 0.0380753081242986 1105 82-83 0.0749039087842931 1105 84-85 0.02632227034185064 1105 86-87 -0.0573169828054958 1105 88-89 0.006779471726233055 1105 90-91 0.039078247801533905 1105 92-93 0.03692168684957409 1105 94-95 0.047678267795713936 1105 96-97 -0.04267442813803157 1105 98-99 -0.08609996281438725 1105 100-101 -0.07011249262327723 1105 102-103 0.017199845131859348 1105 104-105 0.04807095988802956 1105 106-107 0.09729013722970237 1105 108-109 0.003952882622833442 1105 110-111 0.024547760169550514 1105 112-113 0.060928307102333434 1105 114-115 0.11083211735977727 1105 116-117 0.09042014554067634 1105 118-119 0.08540745843372477 1105 120-121 -0.01452301337145201 1105 122-123 0.0408133994981128 1105 124-125 0.11845614915308644 1106 1 0.17663664840173254 1106 2 0.304054747515373 1106 3 0.27273591817505505 1106 4 0.375382799779608 1106 5 0.3236926420694317 1106 6 0.4110713965957302 1106 7 0.41446220894394514 1106 8 0.4667318969794607 1106 9 0.5082290417691979 1106 10-11 0.4253493420144636 1106 12-13 0.50653018551386 1106 14-15 0.6581096624787435 1106 16-17 0.6265650242145782 1106 18-19 0.558391048024383 1106 20-21 0.5180310538056005 1106 22-23 0.5046909447303278 1106 24-25 0.6056453186029245 1106 26-27 0.6131549229087909 1106 28-29 0.6701795798137198 1106 30-31 0.6566862514325251 1106 32-33 0.6213905541659841 1106 34-35 0.6109805203333849 1106 36-37 0.6841278119063361 1106 38-39 0.6624018239708533 1106 40-41 0.6610083598012579 1106 42-43 0.6334189181249883 1106 44-45 0.6204032429793216 1106 46-47 0.5749191638991391 1106 48-49 0.6356130091573462 1106 50-51 0.5847900506891577 1106 52-53 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-0.6887765116678892 2216 98-99 -0.7532110740361162 2216 100-101 -0.7771824215810099 2216 102-103 -0.8416040522743735 2216 104-105 -0.6703574436782453 2216 106-107 -0.6676629496853828 2216 108-109 -0.7116504315712575 2216 110-111 -0.7468262106783037 2216 112-113 -0.7419726456450881 2216 114-115 -0.7564786579026901 2216 116-117 -0.6735331277972065 2216 118-119 -0.7111879734859876 2216 120-121 -0.7708436546061321 2216 122-123 -0.6921929431081573 2216 124-125 -0.705094674952651 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17829.0 3 3148.0 4 1944.0 5 1557.0 6 1746.0 7 1882.0 8 2008.0 9 2234.0 10 2402.0 11 2894.0 12 3320.0 13 3483.0 14 3639.0 15 3842.0 16 3358.0 17 3414.0 18 2940.0 19 2405.0 20 2392.0 21 2191.0 22 2250.0 23 3035.0 24 5767.0 25 10205.0 26 12579.0 27 9152.0 28 8795.0 29 10854.0 30 11893.0 31 14375.0 32 18643.0 33 25601.0 34 39849.0 35 71025.0 36 179992.0 37 446932.0 38 236510.0 39 1095.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82185483315782 18.784012456325094 13.688783952962414 23.705348757554663 2 15.051099886104202 20.12874140886889 40.41606867116461 24.404090033862293 3 19.11288116064191 25.197771029157103 28.341459435483735 27.347888374717254 4 12.8491824415003 16.58054222677319 36.32781603955714 34.242459292169364 5 13.943564675283184 38.67635550396937 32.88786434041671 14.492215480330737 6 32.05893175987076 36.207202789536346 17.366529473103633 14.367335977489265 7 29.194063641717676 32.981156320192135 20.077234186665716 17.747545851424473 8 26.076241725796468 36.62427802684481 19.18475709727674 18.114723150081975 9 26.485307250691047 18.07637678077823 19.166999787369765 36.27131618116096 10-11 25.11394044627684 27.86151470995065 26.14742544523358 20.877119398538927 12-13 25.372050554517422 24.712629026953856 26.894965877485262 23.020354541043467 14-15 22.682998838170043 25.97588406338876 24.505648787512555 26.835468310928633 16-17 22.980160954048515 29.29672147794583 25.116258517303987 22.606859050701672 18-19 23.071515966455124 28.20498872303633 26.511637988004388 22.211857322504162 20-21 23.894989358754863 27.265589392204248 26.377691217919335 22.461730031121547 22-23 23.7978485735255 26.925104879701937 26.303353003858415 22.97369354291415 24-25 23.46582581520538 27.171231386044674 26.229736760670185 23.133206038079756 26-27 22.942134787309286 27.534690877886835 26.153852689352824 23.36932164545105 28-29 23.009717893105723 27.55934677136403 26.369140613385188 23.061794722145056 30-31 23.209003280994867 27.200271518891633 26.305219177593404 23.285506022520096 32-33 23.05574316431554 27.311474434943655 26.091521924738448 23.541260476002364 34-35 23.54343465580124 27.38816057199803 25.95064681959481 23.117757952605928 36-37 23.548441082598284 27.088531352925038 26.012780225820237 23.350247338656445 38-39 23.126698078102674 27.244139241130068 26.17733415118608 23.451828529581178 40-41 23.28497888678722 26.99526338525868 26.295284241485373 23.424473486468727 42-43 23.192829173346517 26.925602800907694 26.151122257649867 23.73044576809592 44-45 23.021804603902144 27.115318445768853 26.414877935847827 23.44799901448118 46-47 23.394396808080394 27.502141087468523 25.856987252033182 23.2464748524179 48-49 23.259901152036733 27.254517786940752 26.0387575098198 23.44682355120272 50-51 23.28277155785435 27.363552022782535 26.142185240628628 23.21149117873449 52-53 23.58364726652971 27.00483941403668 26.113252144006555 23.298261175427058 54-55 23.14997404411396 27.120629874162805 26.321434973920923 23.407961107802315 56-57 23.12714639405798 27.520743873696446 26.095691153755308 23.256418578490266 58-59 23.33548296101442 26.959602293389345 25.98071452604533 23.724200219550905 60-61 23.156015384131777 27.152041065131083 26.434658644398702 23.257284906338434 62-63 23.025910521821512 27.279076584518265 26.382170638140597 23.312842255519634 64-65 22.948597727795146 27.115156402626983 26.300012789065818 23.636233080512056 66-67 22.939085612899717 27.403104266369372 26.08775084294105 23.570059277789863 68-69 22.951785088324957 27.149828448816137 26.485123378761642 23.41326308409726 70-71 22.86309816405754 27.125876749303124 26.32909649946064 23.681928587178692 72-73 22.907560476291017 27.267274130723013 26.123021879496456 23.702143513489517 74-75 22.922537634372063 27.597491693209037 26.039994936489474 23.43997573592943 76-77 23.25162526889577 27.104820923064377 26.149837388151255 23.493716419888603 78-79 23.091335332399225 27.244721862751014 26.22074581755366 23.4431969872961 80-81 23.128912702135036 27.438832697784754 26.264257359788466 23.16799724029174 82-83 23.15110668710997 27.329960952950238 26.248063260384047 23.270869099555743 84-85 23.008801812445228 27.33154144931895 26.195619527542462 23.46403721069336 86-87 23.124791553813637 27.36594122083081 26.288882516011853 23.2203847093437 88-89 23.040390946738096 27.42150453483091 26.101438986162467 23.436665532268528 90-91 22.87223646934263 27.501559252442547 26.375413605518478 23.250790672696343 92-93 22.984179697292355 27.048409259512617 26.44561978077447 23.521791262420557 94-95 22.830204390999114 27.104112461783963 26.52774921783523 23.537933929381694 96-97 23.12220435882097 27.198529611703286 26.379168819338698 23.300097210137046 98-99 22.844473752640713 27.19393660251911 26.224330070719244 23.73725957412094 100-101 22.57480702669657 27.231614271761302 26.601872783127707 23.591705918414426 102-103 22.697679951903773 26.87725052738022 26.6257650465348 23.799304474181206 104-105 22.580020214559838 27.081392280995125 26.611716032593108 23.726871471851926 106-107 22.637226113931167 27.215445073226775 26.41050077350282 23.736828039339237 108-109 22.789938886446045 27.098221398028304 26.36677054059049 23.745069174935157 110-111 22.6659342508074 27.311761901970133 26.538816766035616 23.483487081186855 112-113 22.581766255638485 27.430958439319348 26.59134100440542 23.395934300636746 114-115 22.51426600334831 27.435968127754062 26.397872917979136 23.65189295091849 116-117 22.343384208623664 27.464118328485675 26.61061308201118 23.58188438087948 118-119 22.29929020038052 27.641481937201473 26.287483716586607 23.771744145831402 120-121 22.277962800811128 27.671713483229603 26.56267989825645 23.487643817702818 122-123 22.28842904741614 27.462771405618668 26.52883317621839 23.719966370746796 124-125 22.318158510406448 27.577694161589584 26.308766345905077 23.795380982098894 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 798.0 1 746.5 2 703.5 3 659.0 4 519.0 5 424.0 6 402.5 7 374.5 8 362.0 9 335.0 10 306.5 11 316.0 12 307.5 13 297.5 14 347.0 15 405.5 16 432.5 17 489.5 18 591.5 19 716.5 20 928.0 21 1233.5 22 1600.0 23 2097.0 24 2790.5 25 3700.5 26 4742.0 27 6008.5 28 7399.0 29 9042.5 30 11300.0 31 13798.5 32 16302.5 33 18163.0 34 19635.5 35 21119.0 36 23758.0 37 26726.0 38 28700.0 39 29953.5 40 31192.5 41 33416.5 42 35845.5 43 38275.5 44 40532.5 45 43112.0 46 44203.0 47 44913.0 48 46056.5 49 46126.0 50 46034.5 51 45344.5 52 44704.5 53 45390.5 54 45583.5 55 42972.5 56 38079.0 57 34286.5 58 32789.0 59 29714.0 60 24924.5 61 20412.0 62 16313.5 63 12082.5 64 8914.0 65 7136.0 66 5303.5 67 4155.5 68 3155.0 69 2022.0 70 1508.0 71 1150.5 72 844.0 73 707.5 74 527.5 75 414.5 76 317.5 77 182.5 78 114.0 79 74.5 80 40.0 81 24.5 82 22.0 83 21.0 84 14.0 85 9.5 86 8.5 87 10.5 88 11.0 89 6.5 90 5.0 91 5.5 92 4.5 93 5.5 94 5.5 95 4.0 96 3.0 97 1.5 98 2.5 99 2.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050034828998114136 2 0.20574593520107376 3 0.026673915628875788 4 0.11570023275964594 5 0.024889991335224862 6 0.04221954161640531 7 0.04238943916818159 8 0.10499668699774035 9 0.12147674952003942 10-11 0.04914286685128868 12-13 0.03321497137226253 14-15 0.013379432202381962 16-17 0.03266280432898962 18-19 0.05661835912944494 20-21 0.19287619565402062 22-23 0.11119794763757454 24-25 0.07747328360998318 26-27 0.015078407720144751 28-29 0.00620126063983418 30-31 0.008324980037037667 32-33 0.007220645950491854 34-35 0.009599211675359758 36-37 0.004162490018518834 38-39 0.009684160451247897 40-41 0.00624373502777825 42-43 0.011298187193122547 44-45 0.011468084744898825 46-47 0.017669345384733005 48-49 0.018773679471278816 50-51 0.011722931072563244 52-53 0.015120882108088823 54-55 0.015545625987529519 56-57 0.017499447832956726 58-59 0.019708116006048354 60-61 0.010448699434241152 62-63 0.020812450092594168 64-65 0.033427343311982875 66-67 0.029562174009072528 68-69 0.03546611393329822 70-71 0.027056185120372417 72-73 0.017881717324453355 74-75 0.011128289641346269 76-77 0.012232623727892081 78-79 0.025229786438777414 80-81 0.020727501316706025 82-83 0.022638848774189164 84-85 0.017541922220900796 86-87 0.026971236344484277 88-89 0.029264853293464045 90-91 0.028372891146638577 92-93 0.03406445913114392 94-95 0.027013710732428347 96-97 0.029562174009072528 98-99 0.03516879321768974 100-101 0.022808746325965443 102-103 0.031643419018331946 104-105 0.025951851033826603 106-107 0.033172496984318456 108-109 0.023148541429518 110-111 0.02149204029969928 112-113 0.028415365534582647 114-115 0.014313868737151497 116-117 0.012869739547053127 118-119 0.03134609830272346 120-121 0.025442158378497767 122-123 0.019410795290439865 124-125 0.020515129376985675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1177180.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.31837130996032 #Duplication Level Percentage of deduplicated Percentage of total 1 72.40315404531492 36.43208789264412 2 13.871216883233316 13.959540833030493 3 5.380488761808174 8.12212294037197 4 2.6558794172119824 5.345581066790142 5 1.4426274442149665 3.6295331699973876 6 0.9078760294290081 2.740970589133278 7 0.6279417383470692 2.2117903885818846 8 0.4336328636510078 1.7455759556314245 9 0.3378149181049358 1.5298466834923201 >10 1.7862325875000817 16.18460314537495 >50 0.11001265726600178 3.763987315907235 >100 0.040755138690514045 3.5266619390095926 >500 0.002198406997524174 0.7119690092345348 >1k 1.691082305787826E-4 0.09572907080062766 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.5484632766441836E-4 0.0 24-25 0.0 0.0 0.0 5.096926553288367E-4 0.0 26-27 0.0 0.0 0.0 7.220645950491854E-4 0.0 28-29 0.0 0.0 0.0 0.0014016548021543009 0.0 30-31 0.0 0.0 0.0 0.0033554766475815084 0.0 32-33 0.0 0.0 0.0 0.007263120338435923 0.0 34-35 0.0 0.0 0.0 0.013421906590326034 0.0 36-37 0.0 0.0 0.0 0.023615759696902768 0.0 38-39 0.0 0.0 0.0 0.04086036120219508 0.0 40-41 0.0 0.0 0.0 0.06120559302740448 0.0 42-43 0.0 0.0 0.0 0.08227288944766306 0.0 44-45 0.0 0.0 0.0 0.10826721486943372 0.0 46-47 0.0 0.0 0.0 0.13812670959411474 0.0 48-49 0.0 0.0 0.0 0.16998250055216704 0.0 50-51 0.0 0.0 0.0 0.2107154385905299 0.0 52-53 0.0 0.0 0.0 0.2480928999813113 0.0 54-55 0.0 0.0 0.0 0.28598005402742144 0.0 56-57 0.0 0.0 0.0 0.33745901221563396 0.0 58-59 0.0 0.0 0.0 0.39322788358619754 0.0 60-61 0.0 0.0 0.0 0.45392378395827315 0.0 62-63 0.0 0.0 0.0 0.5140675172870759 0.0 64-65 0.0 0.0 0.0 0.5766747651166346 0.0 66-67 0.0 0.0 0.0 0.6526189707606314 0.0 68-69 0.0 0.0 0.0 0.7407958001325201 0.0 70-71 0.0 0.0 0.0 0.8422671129309027 0.0 72-73 0.0 0.0 0.0 0.9525306240337077 0.0 74-75 0.0 0.0 0.0 1.0573998878676159 0.0 76-77 0.0 0.0 0.0 1.1710188756180022 0.0 78-79 0.0 0.0 0.0 1.3119488948164257 0.0 80-81 0.0 0.0 0.0 1.4726295044088413 0.0 82-83 0.0 0.0 0.0 1.636793013812671 0.0 84-85 0.0 0.0 0.0 1.8002769330093953 0.0 86-87 0.0 0.0 0.0 1.9731052175538148 0.0 88-89 0.0 0.0 0.0 2.1690820435277525 0.0 90-91 0.0 0.0 0.0 2.4046449990655634 0.0 92-93 0.0 0.0 0.0 2.6568154402895052 0.0 94-95 0.0 0.0 0.0 2.914167756842624 0.0 96-97 0.0 0.0 0.0 3.160179411814676 0.0 98-99 0.0 0.0 0.0 3.4370699468220662 0.0 100-101 0.0 0.0 0.0 3.765991607060942 0.0 102-103 0.0 0.0 0.0 4.120652746393924 0.0 104-105 0.0 0.0 0.0 4.486654547307973 0.0 106-107 0.0 0.0 0.0 4.842207648787781 0.0 108-109 0.0 0.0 0.0 5.209865950831649 0.0 110-111 0.0 0.0 0.0 5.619191627448648 0.0 112-113 0.0 0.0 0.0 6.069717460371396 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1450 0.0 59.255302 1 GTATCAA 2525 0.0 51.278004 1 ATCAACG 3000 0.0 41.528927 3 TCAACGC 3065 0.0 40.84444 4 CAACGCA 3150 0.0 39.72199 5 TATCAAC 3345 0.0 38.352493 2 AACGCAG 3235 0.0 38.309925 6 GTACATG 6945 0.0 32.303406 1 TACATGG 7000 0.0 31.45427 2 ACGCAGA 3905 0.0 31.27916 7 CGCAGAG 4075 0.0 29.974262 8 ACATGGG 6970 0.0 29.762688 3 CATGGGG 4080 0.0 25.7156 4 TAAGGTA 350 0.0 25.548616 4 GCAGAGT 4845 0.0 24.58101 9 CATGGGA 3185 0.0 23.957676 4 GTATTGG 445 0.0 22.794058 1 CGTACAC 160 2.5786163E-4 22.354088 3 ATGGGGC 1245 0.0 22.014599 5 GAGTACA 5455 0.0 21.985386 1 >>END_MODULE