FastQCFastQC Report
Fri 27 May 2016
SRR940069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177180
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64100.544521653442974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48310.4103875363156017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29720.25246776193955045No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13160.11179258906879153No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.069.7281341
GTATCAA54050.056.245821
TCAACGC68500.043.758644
ATCAACG68450.043.703553
TATCAAC70200.042.953632
CAACGCA70300.042.6757855
AACGCAG72600.041.3864446
GTGGTAT12800.039.118761
ACGCAGA83750.035.6618427
CGCAGAG84900.035.2488678
TGGTATC14200.034.4121632
GTACATG67150.032.1350021
TACATGG67750.031.665012
ACATGGG65350.031.2777123
GCAGAGT94700.031.1613739
CATGGGG39750.028.9338744
CGCGGAA1600.027.88856182-83
GAGTACT59050.026.7503512-13
GTACTTT59900.026.17210814-15
CAGAGTA93250.026.12702410-11
Produced by FastQC (version 0.11.5)