FastQCFastQC Report
Fri 27 May 2016
SRR940068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248149
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG15330.12282187463195499No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG14710.11785451897169327No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG13660.10944206180512103No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG12960.10383375702740617No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12940.1036735197480429No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC12810.10263197743218158No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.045.3812261
GTATCAA21700.038.220461
ATCAACG23300.034.2837453
TCAACGC23850.033.248534
AACGCAG23950.032.5895586
CAACGCA24350.032.543585
GTACATG48200.032.4335941
ACATGGG49550.030.6786273
TACATGG50400.030.4234522
GTATAGG3400.029.8339581
AGTACAT35500.028.4030062
TATCAAC29150.028.2453172
ACGCAGA29000.026.2981157
CATGGGG37750.025.9021644
GAGTACA42150.024.6315961
CGCAGAG31000.024.4092648
GTATAAC2251.8607352E-723.8671671
GTTCGCG3002.2009772E-1023.8326667
ACTTGAC9600.022.9637688
CTATAGG1300.002018903822.94921
Produced by FastQC (version 0.11.5)