##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940068_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1248149 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06876102132037 34.0 31.0 34.0 31.0 34.0 2 32.14677814908316 34.0 31.0 34.0 31.0 34.0 3 32.27211334544193 34.0 31.0 34.0 31.0 34.0 4 35.479478812225146 37.0 37.0 37.0 35.0 37.0 5 35.55838685926119 37.0 37.0 37.0 35.0 37.0 6 35.64089383559175 37.0 37.0 37.0 35.0 37.0 7 35.67114262800355 37.0 37.0 37.0 35.0 37.0 8 35.66582435270148 37.0 37.0 37.0 35.0 37.0 9 37.401600289709 39.0 39.0 39.0 35.0 39.0 10-11 37.40554493093373 39.0 39.0 39.0 35.0 39.0 12-13 37.37442725187458 39.0 39.0 39.0 35.0 39.0 14-15 38.98632094405396 41.0 40.0 41.0 36.5 41.0 16-17 39.028324342686645 41.0 40.0 41.0 37.0 41.0 18-19 39.05042827418842 41.0 40.0 41.0 36.0 41.0 20-21 39.04246087606528 41.0 40.0 41.0 36.5 41.0 22-23 39.07095386848846 41.0 40.0 41.0 36.0 41.0 24-25 39.102706087173885 41.0 40.0 41.0 36.0 41.0 26-27 39.11138734237659 41.0 40.0 41.0 36.0 41.0 28-29 39.04876260766943 41.0 40.0 41.0 36.0 41.0 30-31 38.97051313585157 41.0 40.0 41.0 35.0 41.0 32-33 38.90249882025303 41.0 40.0 41.0 35.0 41.0 34-35 38.806704568124474 41.0 40.0 41.0 35.0 41.0 36-37 38.66717114703452 41.0 39.5 41.0 35.0 41.0 38-39 38.56767541375268 41.0 39.0 41.0 35.0 41.0 40-41 38.44651199496214 41.0 39.0 41.0 34.0 41.0 42-43 38.28327387194958 41.0 38.5 41.0 34.0 41.0 44-45 38.12435334242947 40.5 38.0 41.0 33.5 41.0 46-47 37.93243635174967 40.0 38.0 41.0 33.0 41.0 48-49 37.756312747917114 40.0 38.0 41.0 33.0 41.0 50-51 37.55649565877151 40.0 37.0 41.0 33.0 41.0 52-53 37.609525385190395 40.0 37.0 41.0 33.0 41.0 54-55 37.34301834156018 40.0 36.5 41.0 33.0 41.0 56-57 37.181413837610734 40.0 36.0 41.0 32.0 41.0 58-59 36.9574177441956 39.5 35.0 41.0 32.0 41.0 60-61 36.67589125977747 39.0 35.0 41.0 32.0 41.0 62-63 36.373846792330085 39.0 35.0 41.0 31.5 41.0 64-65 36.02921085543473 37.5 35.0 41.0 31.0 41.0 66-67 35.68628024378499 37.0 35.0 40.0 31.0 41.0 68-69 35.336203049475664 36.5 35.0 39.5 31.0 41.0 70-71 34.96067937401705 36.0 35.0 39.0 31.0 41.0 72-73 34.59499466810453 35.5 35.0 38.5 30.0 40.5 74-75 34.2348742017179 35.0 35.0 37.0 30.0 39.5 76-77 33.88003475546589 35.0 35.0 37.0 30.0 39.0 78-79 33.56640513272053 35.0 34.0 36.5 30.0 39.0 80-81 33.27985961611955 35.0 34.0 36.0 29.0 37.0 82-83 33.00924849517165 35.0 34.0 36.0 29.0 37.0 84-85 32.80426575673257 35.0 34.0 35.0 29.0 36.5 86-87 32.6129692849171 35.0 34.0 35.0 29.0 36.0 88-89 32.47225130973946 35.0 34.0 35.0 28.0 36.0 90-91 32.34389724303749 35.0 34.0 35.0 28.0 36.0 92-93 32.217529718006425 35.0 34.0 35.0 27.0 35.5 94-95 32.11227705987025 35.0 34.0 35.0 27.0 35.0 96-97 32.018246619594294 35.0 34.0 35.0 27.0 35.0 98-99 31.939367415268528 35.0 33.5 35.0 27.0 35.0 100-101 31.834564222700976 35.0 33.0 35.0 25.5 35.0 102-103 31.179218186290257 34.0 32.0 35.0 26.0 35.0 104-105 32.07999165163774 35.0 34.0 35.0 27.0 35.0 106-107 32.18575666847468 35.0 34.0 35.0 28.0 35.0 108-109 32.15379534013968 35.0 34.0 35.0 27.0 35.0 110-111 32.09934070371406 35.0 34.0 35.0 27.0 35.0 112-113 32.03416178677386 35.0 34.0 35.0 27.0 35.0 114-115 31.951246605974127 35.0 34.0 35.0 27.0 35.0 116-117 31.84575920022369 35.0 34.0 35.0 26.0 35.0 118-119 31.719039954364423 35.0 34.0 35.0 25.0 35.0 120-121 31.605226218985074 35.0 34.0 35.0 25.0 35.0 122-123 31.431448088329198 35.0 34.0 35.0 25.0 35.0 124-125 30.093732398936346 34.5 31.5 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0199847094763399 1101 2 0.1328331340083153 1101 3 0.2542640425847509 1101 4 0.07888009114551409 1101 5 0.19794681931858804 1101 6 0.1705644066315699 1101 7 0.09338420747355514 1101 8 0.166736551080227 1101 9 0.22492485567771325 1101 10-11 0.18952812398791963 1101 12-13 0.22597806810199472 1101 14-15 0.2858737787150929 1101 16-17 0.2634969855497147 1101 18-19 0.24728305452192245 1101 20-21 0.16844723942594442 1101 22-23 0.2046120610904012 1101 24-25 0.25905926848964356 1101 26-27 0.2839004495784039 1101 28-29 0.3039452701094092 1101 30-31 0.3584773111343722 1101 32-33 0.3445291170910423 1101 34-35 0.4031724363440077 1101 36-37 0.4179160540501883 1101 38-39 0.3993875275449099 1101 40-41 0.39891583831946775 1101 42-43 0.4680316855670199 1101 44-45 0.3927604247092944 1101 46-47 0.36813181879052337 1101 48-49 0.3375805248707735 1101 50-51 0.4078762309172248 1101 52-53 0.38651893111700986 1101 54-55 0.4935551026925893 1101 56-57 0.40377788369952583 1101 58-59 0.4921137979883028 1101 60-61 0.41882285959120225 1101 62-63 0.3375121178335121 1101 64-65 0.4220010590066039 1101 66-67 0.42652061267030206 1101 68-69 0.4486947786491484 1101 70-71 0.5270558484154932 1101 72-73 0.5595174729502688 1101 74-75 0.5264950427737318 1101 76-77 0.5263565328343063 1101 78-79 0.5207961254887081 1101 80-81 0.5830811814728349 1101 82-83 0.525033254630074 1101 84-85 0.46238668063831057 1101 86-87 0.43036988576135116 1101 88-89 0.5543297502861009 1101 90-91 0.49139484653225907 1101 92-93 0.5404398669904111 1101 94-95 0.5372477908268749 1101 96-97 0.5873184641114548 1101 98-99 0.5713411123310266 1101 100-101 0.5014875370284493 1101 102-103 0.5154042554041034 1101 104-105 0.5249403600920957 1101 106-107 0.6390562932900039 1101 108-109 0.7098139461204767 1101 110-111 0.7848479484550026 1101 112-113 0.7326944868001846 1101 114-115 0.7247777955102919 1101 116-117 0.694940346833004 1101 118-119 0.6701233476713071 1101 120-121 0.7102924188262101 1101 122-123 0.6467033185749642 1101 124-125 0.6719343285312256 1102 1 -0.03864068493133033 1102 2 0.0917425588041283 1102 3 0.11437699857862782 1102 4 0.006141540396022549 1102 5 0.030262592169520985 1102 6 0.16297699390650422 1102 7 0.1527003210909612 1102 8 0.09176223979814324 1102 9 0.19488884991574196 1102 10-11 0.13857609944573568 1102 12-13 0.06682787582484195 1102 14-15 0.22614450215051107 1102 16-17 0.2374574400158238 1102 18-19 0.21458128380482577 1102 20-21 -0.01783566323950936 1102 22-23 0.11126523124660537 1102 24-25 0.13808192049270218 1102 26-27 0.2831694858042013 1102 28-29 0.26505720992804527 1102 30-31 0.32895857925809935 1102 32-33 0.3351584764631923 1102 34-35 0.3332818446920456 1102 36-37 0.3569610680329873 1102 38-39 0.33917990933634456 1102 40-41 0.34559594608741406 1102 42-43 0.33775543725280954 1102 44-45 0.3251003442989031 1102 46-47 0.35278661010277546 1102 48-49 0.33599480154252603 1102 50-51 0.4147427410092064 1102 52-53 0.4471854248486977 1102 54-55 0.5407873483874042 1102 56-57 0.39918453533863385 1102 58-59 0.2593794816668975 1102 60-61 0.21548326064578305 1102 62-63 0.21933745379907776 1102 64-65 0.1829124759083598 1102 66-67 0.22213356144640528 1102 68-69 0.20511302116026542 1102 70-71 0.3028247273812781 1102 72-73 0.198224738800171 1102 74-75 0.2944411658004924 1102 76-77 0.24656625950809286 1102 78-79 0.3158187477457872 1102 80-81 0.2033990872856748 1102 82-83 0.23262871626803872 1102 84-85 0.26810648310748775 1102 86-87 0.1788391706285708 1102 88-89 0.19270215511317446 1102 90-91 0.14768876833444722 1102 92-93 0.17805112461992678 1102 94-95 0.19153910810582886 1102 96-97 0.11123904583788402 1102 98-99 0.1665993402666217 1102 100-101 0.19516909666280569 1102 102-103 0.30366316618496825 1102 104-105 0.23040089132909713 1102 106-107 0.25149282608079915 1102 108-109 0.2858932331617723 1102 110-111 0.3362818141685153 1102 112-113 0.3535828155702845 1102 114-115 0.28041678076984766 1102 116-117 0.398683922466315 1102 118-119 0.44696898745708324 1102 120-121 0.46314967354324565 1102 122-123 0.5393271023251671 1102 124-125 0.49403060665266096 1103 1 0.07419047955787761 1103 2 0.28251812091992434 1103 3 0.2735582663830556 1103 4 0.18297342524823534 1103 5 0.20636334303593884 1103 6 0.3599350973871651 1103 7 0.3122396162036978 1103 8 0.330084345464023 1103 9 0.2981433224566459 1103 10-11 0.3708005390532918 1103 12-13 0.39065022017558704 1103 14-15 0.5543730381764149 1103 16-17 0.5213668483358518 1103 18-19 0.45091851364716007 1103 20-21 0.1943866394060123 1103 22-23 0.28663764858150387 1103 24-25 0.3527804709775708 1103 26-27 0.31666098017830535 1103 28-29 0.38357394285776536 1103 30-31 0.37596980520219603 1103 32-33 0.4133747335741873 1103 34-35 0.45880660673959284 1103 36-37 0.47533993244447004 1103 38-39 0.4684637427015659 1103 40-41 0.47890144721363015 1103 42-43 0.5069261408820225 1103 44-45 0.47043982829150366 1103 46-47 0.501840142725051 1103 48-49 0.48385986445366314 1103 50-51 0.43965644393357195 1103 52-53 0.5802998488254048 1103 54-55 0.6202103858721273 1103 56-57 0.5746082225287523 1103 58-59 0.6020383277418659 1103 60-61 0.5114384999079817 1103 62-63 0.5630686584424893 1103 64-65 0.5383803844257073 1103 66-67 0.4869186675103876 1103 68-69 0.5429764124156122 1103 70-71 0.5191492647031524 1103 72-73 0.5037663638908683 1103 74-75 0.5043608487718387 1103 76-77 0.5115398576868486 1103 78-79 0.43495287313375997 1103 80-81 0.33723042755724464 1103 82-83 0.45291029548297956 1103 84-85 0.5144195773423448 1103 86-87 0.5135878104694669 1103 88-89 0.4793043079300858 1103 90-91 0.4823075101808172 1103 92-93 0.41758388821160963 1103 94-95 0.46492527093199953 1103 96-97 0.47135312341995217 1103 98-99 0.42239994275204396 1103 100-101 0.4378980402625139 1103 102-103 0.34399293906382056 1103 104-105 0.5201854373022243 1103 106-107 0.5553065708827276 1103 108-109 0.5706759523770231 1103 110-111 0.5406841713755028 1103 112-113 0.6062359228681728 1103 114-115 0.5807482683883904 1103 116-117 0.6373298037305055 1103 118-119 0.5163750154133737 1103 120-121 0.6608959971930339 1103 122-123 0.5425879082011278 1103 124-125 0.48770653463703795 1104 1 -0.07622399565974547 1104 2 0.0782294678123776 1104 3 0.1055995314951872 1104 4 0.10732916456460373 1104 5 0.05838606316888928 1104 6 0.15659902790936542 1104 7 0.13035441266494985 1104 8 0.25475423470084024 1104 9 0.2509726985143672 1104 10-11 0.2645165272574701 1104 12-13 0.1789017731909226 1104 14-15 0.23986969906677302 1104 16-17 0.26256431689552073 1104 18-19 0.23550540650176544 1104 20-21 0.1692495788358812 1104 22-23 0.18384855465067318 1104 24-25 0.20192897268513121 1104 26-27 0.11635620408612368 1104 28-29 0.18668174694981587 1104 30-31 0.18770913170530434 1104 32-33 0.20726198415434993 1104 34-35 0.24884932099491408 1104 36-37 0.21293987772158118 1104 38-39 0.1566737468564483 1104 40-41 0.12447953373659004 1104 42-43 0.16162977590062155 1104 44-45 0.11140573795820785 1104 46-47 0.16062772670948533 1104 48-49 0.1585411542924291 1104 50-51 0.2525953671087109 1104 52-53 0.1742496760429475 1104 54-55 0.18413482774932532 1104 56-57 0.04925961745574625 1104 58-59 0.11238754084698854 1104 60-61 0.07559222055776615 1104 62-63 0.18441919908436688 1104 64-65 0.1991453394820013 1104 66-67 0.18352838183663067 1104 68-69 0.1980461334302106 1104 70-71 0.21597559046647774 1104 72-73 0.1969432302960783 1104 74-75 0.18308048516863096 1104 76-77 0.13840195600564442 1104 78-79 0.11753858032576403 1104 80-81 0.06615831494969626 1104 82-83 0.07753407561857273 1104 84-85 0.15923635700163885 1104 86-87 0.10989756229103165 1104 88-89 0.20733028103479967 1104 90-91 0.11952135507182504 1104 92-93 0.04419390655391453 1104 94-95 0.01770119083390398 1104 96-97 -0.05688870108171784 1104 98-99 0.023126794463152578 1104 100-101 0.04490282344839969 1104 102-103 -0.005963738648766537 1104 104-105 0.07987392218755218 1104 106-107 0.036162426738577835 1104 108-109 0.11522063766973645 1104 110-111 0.12314472529274667 1104 112-113 0.13662232727032375 1104 114-115 0.07247649830559766 1104 116-117 0.09629397037183551 1104 118-119 0.054123675209275746 1104 120-121 0.01902310630439885 1104 122-123 -0.028206029718795378 1104 124-125 -0.025772777070617536 1105 1 -0.02152481800975181 1105 2 0.05627173273988717 1105 3 0.06728487022970597 1105 4 -0.027286625788889296 1105 5 -0.08336584907482347 1105 6 0.008715601337414114 1105 7 -0.022780888011332934 1105 8 0.031095158582751026 1105 9 0.08118671725297588 1105 10-11 -0.037443512407485 1105 12-13 -0.012723873352754822 1105 14-15 0.029346394281169808 1105 16-17 0.027312491479762002 1105 18-19 0.0778915685600623 1105 20-21 -0.045964464247013836 1105 22-23 0.009031015079038696 1105 24-25 -0.019460810180056853 1105 26-27 4.364201335533835E-4 1105 28-29 0.008139531522360244 1105 30-31 0.06706817909713436 1105 32-33 0.09884024048670881 1105 34-35 0.06334707401725126 1105 36-37 0.03652697589883758 1105 38-39 0.021903370282792878 1105 40-41 0.04794637113785427 1105 42-43 0.04388254749765963 1105 44-45 -0.07191444946034409 1105 46-47 -0.04196258653656315 1105 48-49 -0.03580821218808694 1105 50-51 -0.02408799973858322 1105 52-53 -0.06411213199098142 1105 54-55 0.025065201466581755 1105 56-57 -0.026238507222714702 1105 58-59 -0.07393632580475185 1105 60-61 -0.042553036931749944 1105 62-63 0.013675675881827942 1105 64-65 -0.07737451490670821 1105 66-67 -0.05400288028479139 1105 68-69 -0.103878201281006 1105 70-71 -0.04947572826352342 1105 72-73 -0.048520211888678944 1105 74-75 -0.0647589593921225 1105 76-77 -0.045586887819268895 1105 78-79 -0.05536306388735568 1105 80-81 -0.06588550152034856 1105 82-83 -0.07085490063926159 1105 84-85 -0.1108261753559745 1105 86-87 -0.10937873075575766 1105 88-89 -0.05020711584685955 1105 90-91 -0.06358825426728743 1105 92-93 -0.06657706077443493 1105 94-95 -0.17173184695978705 1105 96-97 -0.1395347140555998 1105 98-99 -0.12600630548962144 1105 100-101 -0.12583258499192596 1105 102-103 -0.09914758183261085 1105 104-105 -0.03170892710021178 1105 106-107 -0.026713185317355226 1105 108-109 -0.04288454912396844 1105 110-111 -0.07445014033490338 1105 112-113 -0.09110183897842461 1105 114-115 -0.10865838364895808 1105 116-117 -0.16413552776258555 1105 118-119 -0.20132133023573218 1105 120-121 -0.09673681683257129 1105 122-123 -0.18099036692935755 1105 124-125 -0.13439738979359106 1106 1 0.0019625256664497215 1106 2 0.07968191796692992 1106 3 0.05335348357547076 1106 4 0.09187719484810941 1106 5 0.06664498428318666 1106 6 0.06136650066200389 1106 7 0.04151314335396705 1106 8 0.18772914851629707 1106 9 0.14123157826644928 1106 10-11 0.06654542353852833 1106 12-13 0.10545520283459098 1106 14-15 0.1528610941347992 1106 16-17 0.17528447127204316 1106 18-19 0.11594354373842464 1106 20-21 0.06501042886125674 1106 22-23 0.19001589981678535 1106 24-25 0.1949769385390283 1106 26-27 0.27630827164016836 1106 28-29 0.23684496862107096 1106 30-31 0.22097668320523667 1106 32-33 0.22616571041284317 1106 34-35 0.2186187107272204 1106 36-37 0.34267923212100726 1106 38-39 0.28801389859214765 1106 40-41 0.27821056478529727 1106 42-43 0.3102736519857814 1106 44-45 0.38180645829712034 1106 46-47 0.3020884970330471 1106 48-49 0.3288278103640039 1106 50-51 0.33625442790003746 1106 52-53 0.19861082864885304 1106 54-55 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-0.6627291703845835 2216 122-123 -0.6815913318382698 2216 124-125 -0.70338302746568 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9848.0 3 1616.0 4 1145.0 5 1661.0 6 2994.0 7 2851.0 8 2291.0 9 2481.0 10 2542.0 11 2742.0 12 3018.0 13 2819.0 14 2995.0 15 3199.0 16 3019.0 17 3621.0 18 2591.0 19 2356.0 20 2437.0 21 2130.0 22 2184.0 23 3065.0 24 4988.0 25 7649.0 26 8814.0 27 7631.0 28 8412.0 29 10150.0 30 12016.0 31 15597.0 32 20379.0 33 28762.0 34 44157.0 35 86446.0 36 226039.0 37 493745.0 38 209434.0 39 325.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8471496879747 18.012657365360184 13.12887025599301 25.011322690672106 2 16.395103758094496 19.955277029495484 38.00352484453348 25.646094367876543 3 19.221611831692492 23.266617781998423 28.70627927570552 28.805491110603565 4 13.639896404225643 16.591204946545833 34.860667854269714 34.90823079495881 5 15.51353023387014 36.63508757308442 32.26805313365473 15.583329059390708 6 33.622464723539146 34.460203022494376 17.052206356776168 14.865125897190307 7 29.79308852348644 31.876309978479284 19.960324934776597 18.370276563257683 8 26.774345673190286 34.05835807560468 19.68971507490051 19.477581176304522 9 26.772993319085852 16.569276779812213 19.146980111284392 37.510749789817545 10-11 26.281436499383403 25.749925154770313 26.159439776943184 21.8091985689031 12-13 25.821874304994818 23.14760457386039 26.687397439776234 24.34312368136855 14-15 23.554153101314053 24.921492566734322 23.832484889472592 27.69186944247903 16-17 24.424701240559955 27.627684547218458 24.54900568466423 23.398608527557357 18-19 24.15429950691687 26.36980673482132 25.816841782923177 23.65905197533863 20-21 24.896229921262368 25.964995508848652 25.188816993007613 23.949957576881367 22-23 24.808656256604543 25.638076492581458 25.158639352986444 24.394627897827554 24-25 24.722431243728522 25.984885506771693 25.109093190540356 24.18359005895943 26-27 24.26539258992157 26.231637569237858 25.072267796508307 24.43070204433226 28-29 24.065161748580902 26.32995035909631 25.41982038574159 24.185067506581195 30-31 24.302215035834234 25.874682551860378 25.331590331548266 24.491512080757126 32-33 24.3315615139681 25.980723652266164 25.13424856857614 24.5534662651896 34-35 24.851085787726937 26.245478889196928 24.52072619816447 24.38270912491166 36-37 24.991406849041773 25.80753585286923 24.62464555756129 24.576411740527703 38-39 24.40475165050185 25.872986175406908 24.96315191267514 24.759110261416094 40-41 24.622277703307418 25.612844761484304 25.234621692801625 24.530255842406653 42-43 25.02292654491259 25.753451000895428 24.664715556597216 24.558906897594763 44-45 24.434400923708328 25.886949895553972 24.794733708703355 24.883915472034346 46-47 24.444350956482022 26.135187600290884 24.620562410226515 24.79989903300058 48-49 24.711059434050714 25.737005788026647 24.810144231886476 24.741790546036167 50-51 24.687930814130283 25.824428756238476 24.679958060914238 24.807682368717 52-53 24.977894055734627 25.580313589897173 24.713784613782007 24.728007740586193 54-55 24.646504226083447 25.478539445048092 24.855444548079912 25.01951178078855 56-57 24.465008119383995 25.95826961162781 24.886863505802967 24.689858763185235 58-59 24.6760890687448 25.198281129486244 24.95767932447187 25.167950477297087 60-61 24.488283329841163 25.313664460655776 25.30897707145544 24.889075138047616 62-63 24.33040373908915 25.66232402901681 24.941070881222217 25.06620135067183 64-65 24.424087965634417 25.320694684875296 25.010859460152595 25.244357889337692 66-67 24.16660489139908 25.7596454567129 24.850407015533307 25.22334263635471 68-69 24.127196959891194 25.427610474638314 25.298735251581185 25.146457313889307 70-71 24.200754427876166 25.392901133404873 25.259670164196653 25.14667427452231 72-73 24.266786650250392 25.495443121250595 24.997145314890798 25.24062491360821 74-75 24.307448843661422 25.67390150339039 24.955439148245713 25.063210504702475 76-77 24.387124792970575 25.43955942696018 25.01332119668817 25.159994583381074 78-79 24.481452661794865 25.45163283410572 25.167666911619165 24.899247592480254 80-81 24.38466287927809 25.942102557589124 25.10406370313327 24.569170859999513 82-83 24.68251344772666 25.574340635674293 24.99984974607086 24.743296170528193 84-85 24.457935159361075 25.30489331788917 24.952471540018262 25.284699982731496 86-87 24.670732728619814 25.034770960447855 25.24818511814914 25.04631119278319 88-89 24.535130528094925 25.207716162050463 25.196496383825405 25.060656926029207 90-91 24.34000474430443 25.73765118463358 25.17013780922126 24.752206261840726 92-93 24.308829257424236 25.069605958943303 25.330357815502936 25.291206968129526 94-95 24.138492016425552 25.242704830535367 25.40542966408401 25.213373488955064 96-97 24.493627163414533 25.305338372618603 25.24006327957455 24.96097118439232 98-99 24.123459422029807 25.274054174487436 25.211821957353497 25.390664446129264 100-101 23.871133956593493 25.501872945799764 25.374178470158476 25.25281462744826 102-103 24.072308728602643 24.9140779867742 25.62004698712963 25.393566297493525 104-105 23.66536428333589 25.243570792901814 25.673513562029765 25.417551361732528 106-107 23.91808216147941 25.49056773136668 25.341858627372094 25.24949147978181 108-109 23.98228826170612 25.417730810747567 25.242111977741327 25.357868949804992 110-111 23.6231953159652 25.758745603107947 25.575878166373307 25.042180914553548 112-113 23.731761089201665 25.64795365972305 25.39663395076025 25.22365130031503 114-115 23.207012573047635 25.87314611450279 25.468734850603415 25.451106461846155 116-117 23.395421660295174 25.66880634483621 25.762595882875654 25.173176111992962 118-119 23.30375289312894 25.879358927506686 25.38596423740006 25.430923941964313 120-121 23.28375325009927 25.975069850338162 25.561156760867622 25.180020138694942 122-123 23.35241027334087 25.921386553241412 25.464549665886445 25.26165350753128 124-125 23.39333164519634 25.93699616322789 25.37846856058542 25.29120363099035 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 995.0 1 685.5 2 367.0 3 381.0 4 349.5 5 252.0 6 219.5 7 217.5 8 205.5 9 213.5 10 196.5 11 175.0 12 162.5 13 163.5 14 175.5 15 172.0 16 206.5 17 255.0 18 299.0 19 384.0 20 528.5 21 740.0 22 990.5 23 1278.5 24 1523.0 25 1951.5 26 2497.0 27 3029.5 28 3960.0 29 5372.0 30 6753.0 31 8522.0 32 10356.0 33 11111.5 34 11644.5 35 12422.5 36 14663.0 37 17421.0 38 20219.5 39 22728.0 40 25265.0 41 30337.5 42 35082.5 43 38817.5 44 42311.0 45 46129.5 46 48169.0 47 48495.5 48 51475.5 49 54055.5 50 52703.0 51 50858.0 52 52487.0 53 55046.5 54 54577.0 55 51841.0 56 46780.0 57 42024.0 58 40093.0 59 36793.5 60 33135.0 61 29368.5 62 24910.0 63 20338.0 64 16367.5 65 13454.0 66 9965.0 67 8106.5 68 6300.0 69 4034.0 70 3277.5 71 2639.0 72 2193.0 73 1922.0 74 1613.0 75 1354.0 76 914.5 77 555.5 78 333.5 79 190.5 80 118.0 81 79.5 82 56.0 83 43.0 84 33.0 85 32.0 86 26.0 87 16.5 88 16.5 89 14.0 90 14.5 91 11.0 92 8.5 93 9.0 94 7.0 95 8.5 96 7.5 97 7.0 98 7.5 99 9.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05239759035179294 2 0.21664080169915612 3 0.025157252860035142 4 0.110563722760664 5 0.02219286319181444 6 0.04037979439954685 7 0.04118098079636325 8 0.10375363838772454 9 0.12923136580648623 10-11 0.04602815849710251 12-13 0.03256822703058689 14-15 0.014261117863332022 16-17 0.03252816771074607 18-19 0.05544209865969528 20-21 0.1895607014867616 22-23 0.11012307024241495 24-25 0.07495098742217476 26-27 0.014942126300625969 28-29 0.006850143692780269 30-31 0.008853109684821284 32-33 0.0072907962110292926 34-35 0.009213643563388666 36-37 0.004967355660261716 38-39 0.00957417744195605 40-41 0.006089016615804683 42-43 0.011336787514952141 44-45 0.010896134996703118 46-47 0.017345685491075184 48-49 0.01826704984741405 50-51 0.011897617992723626 52-53 0.014541533102217764 54-55 0.014821948341103505 56-57 0.0167848550133037 58-59 0.018948058284707997 60-61 0.00957417744195605 62-63 0.020149837879932603 64-65 0.03116615083615818 66-67 0.027761108649688455 68-69 0.03449107438294627 70-71 0.025317490139398418 72-73 0.016063787256168933 74-75 0.010936194316543938 76-77 0.011056372276066399 78-79 0.023074168228312486 80-81 0.018867939645026355 82-83 0.02095102427674901 84-85 0.01702521093234862 86-87 0.027280396811598617 88-89 0.0282418204877783 90-91 0.026999981572712873 92-93 0.032928760909154274 94-95 0.026999981572712873 96-97 0.028281879807619124 98-99 0.03228781179170115 100-101 0.019909481960887685 102-103 0.028882769605231427 104-105 0.024716600341786115 106-107 0.032928760909154274 108-109 0.022112744552132798 110-111 0.020069719240250965 112-113 0.02691986293303123 114-115 0.012979219628425772 116-117 0.011376846834792962 118-119 0.029243303483798812 120-121 0.024235888503696273 122-123 0.017626100729960924 124-125 0.018026693928369128 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1248149.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.89277036166491 #Duplication Level Percentage of deduplicated Percentage of total 1 58.31288379849651 16.848207607164095 2 14.059106241380018 8.124130562448855 3 7.34333250961475 6.365076596689423 4 4.66232081234685 5.388294583341941 5 3.226131633617152 4.660594022330161 6 2.263137453670893 3.92329864474777 7 1.6860742390367733 3.4100749060845885 8 1.2673263713476757 2.9293255856504365 9 0.9832655587591468 2.5568339394385946 >10 5.731504224136365 29.088498130225055 >50 0.28968370484468775 5.747563659904016 >100 0.16495827429870144 8.697252607700024 >500 0.007775810719305373 1.3409273899366176 >1k 0.0024993677312052987 0.9199217643383988 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 1533 0.12282187463195499 No Hit GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG 1471 0.11785451897169327 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1366 0.10944206180512103 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 1296 0.10383375702740617 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1294 0.1036735197480429 No Hit GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC 1281 0.10263197743218158 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.6023727936328116E-4 0.0 18-19 0.0 0.0 0.0 1.6023727936328116E-4 0.0 20-21 0.0 0.0 0.0 1.6023727936328116E-4 0.0 22-23 0.0 0.0 0.0 4.005931984082029E-4 0.0 24-25 0.0 0.0 0.0 6.810084372939449E-4 0.0 26-27 0.0 0.0 0.0 0.0016424321134736316 0.0 28-29 0.0 0.0 0.0 0.00416616926344531 0.0 30-31 0.0 0.0 0.0 0.010415423158613275 0.0 32-33 0.0 0.0 0.0 0.02499701558067186 0.0 34-35 0.0 0.0 0.0 0.04542726869949021 0.0 36-37 0.0 0.0 0.0 0.07719430933326069 0.0 38-39 0.0 0.0 0.0 0.1253856711017675 0.0 40-41 0.0 0.0 0.0 0.17557999886231532 0.0 42-43 0.0 0.0 0.0 0.22645533506015708 0.0 44-45 0.0 0.0 0.0 0.28281879807619126 0.0 46-47 0.0 0.0 0.0 0.35508581106903103 0.0 48-49 0.0 0.0 0.0 0.42326677343810715 0.0 50-51 0.0 0.0 0.0 0.49937948113566566 0.0 52-53 0.0 0.0 0.0 0.5750114769951344 0.0 54-55 0.0 0.0 0.0 0.6590559300211754 0.0 56-57 0.0 0.0 0.0 0.7602057126192465 0.0 58-59 0.0 0.0 0.0 0.868205638910098 0.0 60-61 0.0 0.0 0.0 0.986140276521473 0.0 62-63 0.0 0.0 0.0 1.1057974648860032 0.0 64-65 0.0 0.0 0.0 1.2388745253972082 0.0 66-67 0.0 0.0 0.0 1.3995524572787383 0.0 68-69 0.0 0.0 0.0 1.5734099053878983 0.0 70-71 0.0 0.0 0.0 1.7607272849635742 0.0 72-73 0.0 0.0 0.0 1.9584200283780224 0.0 74-75 0.0 0.0 0.0 2.172416914967684 0.0 76-77 0.0 0.0 0.0 2.4039197243277846 0.0 78-79 0.0 0.0 0.0 2.6611406170256915 0.0 80-81 0.0 0.0 0.0 2.9545751348597 0.0 82-83 0.0 0.0 0.0 3.2678790753347555 0.0 84-85 0.0 0.0 0.0 3.593681523600147 0.0 86-87 0.0 0.0 0.0 3.941756953697035 0.0 88-89 0.0 0.0 0.0 4.315109814613479 0.0 90-91 0.0 0.0 0.0 4.728401817411223 0.0 92-93 0.0 0.0 0.0 5.1629252597246005 0.0 94-95 0.0 0.0 0.0 5.625209810687666 0.0 96-97 0.0 0.0 0.0 6.109046275725094 0.0 98-99 0.0 0.0 0.0 6.629296662497827 0.0 100-101 0.0 0.0 0.0 7.202585588739806 0.0 102-103 0.0 0.0 0.0 7.81032553004489 0.0 104-105 0.0 0.0 0.0 8.437694538071977 0.0 106-107 0.0 0.0 0.0 9.080846918116347 0.0 108-109 0.0 0.0 0.0 9.760573457175386 0.0 110-111 0.0 0.0 0.0 10.493979484821123 0.0 112-113 0.0 0.0 0.0 11.249978968857084 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1420 0.0 45.381226 1 GTATCAA 2170 0.0 38.22046 1 ATCAACG 2330 0.0 34.283745 3 TCAACGC 2385 0.0 33.24853 4 AACGCAG 2395 0.0 32.589558 6 CAACGCA 2435 0.0 32.54358 5 GTACATG 4820 0.0 32.433594 1 ACATGGG 4955 0.0 30.678627 3 TACATGG 5040 0.0 30.423452 2 GTATAGG 340 0.0 29.833958 1 AGTACAT 3550 0.0 28.403006 2 TATCAAC 2915 0.0 28.245317 2 ACGCAGA 2900 0.0 26.298115 7 CATGGGG 3775 0.0 25.902164 4 GAGTACA 4215 0.0 24.631596 1 CGCAGAG 3100 0.0 24.409264 8 GTATAAC 225 1.8607352E-7 23.867167 1 GTTCGCG 300 2.2009772E-10 23.832666 7 ACTTGAC 960 0.0 22.963768 8 CTATAGG 130 0.0020189038 22.9492 1 >>END_MODULE