FastQCFastQC Report
Fri 27 May 2016
SRR940068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248149
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34770.2785725101730643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27740.22224910647687096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16720.13395836554770305No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG16630.13323729779056828No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG15330.12282187463195499No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG14360.11505036658283586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.065.179791
GTATCAA39650.052.3497771
ATCAACG49050.041.5811163
TCAACGC50550.040.347254
CAACGCA51150.039.806835
AACGCAG51850.039.368466
TATCAAC55300.037.3144872
ACGCAGA61550.033.2502027
GTACATG48100.032.6129041
CGCAGAG64650.031.4717928
TATCGTT953.2749498E-431.3345575
ACATGGG49700.030.957963
TACATGG51200.030.6346042
CGCGAAA400.00536554729.747555114-115
GCAGAGT68700.029.4432749
CATGGGG38000.027.9347924
GAGTACT43900.026.08735812-13
CAGAGTA67500.026.00052610-11
GTGGTAT9350.025.5168571
GTACTTT45450.025.13224214-15
Produced by FastQC (version 0.11.5)