FastQCFastQC Report
Fri 27 May 2016
SRR940067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914270
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17040.1863782033753705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12100.051.2752231
GTATCAA18950.049.7401921
TCAACGC22550.039.927814
ATCAACG22900.039.3110923
CAACGCA23500.038.542075
TATCAAC25100.037.3131332
AACGCAG24150.037.2579656
GTACATG49200.035.527291
TACATGG50450.034.172162
ACATGGG54600.030.4638823
ACGCAGA30400.029.598027
CGCAGAG31400.028.4656418
CGATACC1800.028.085691118-119
CATGGGG31000.027.1208044
ATACCGT900.009459526.483596
GCAGAGT35900.024.3902139
CATGGGA28000.024.0638754
GTGGTAT7300.022.8820421
GTCCAAG7000.022.1582221
TACCGTG1350.002533034222.0696547
Produced by FastQC (version 0.11.5)