FastQCFastQC Report
Fri 27 May 2016
SRR940067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914270
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60860.6656676911634418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46210.5054305620877859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.30220831920548635No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13250.1449243658875387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.10423616655911272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23000.075.9626161
GTATCAA43800.061.3991781
GTGGTAT7250.047.7034871
TCAACGC55900.047.4584054
ATCAACG56450.046.996013
TATCAAC57000.046.647132
CAACGCA57350.046.3875925
AACGCAG59400.044.8745966
TATAACG1002.1910273E-741.73142
ACGCAGA68100.039.2269447
CGCAGAG70100.038.02298
TGGTATC9300.037.8210872
GCAGAGT75750.034.794169
TAACGCA1209.1449147E-734.776174
GTACATG45000.033.9224781
TACATGG45500.032.7562032
TACGGTA953.2483856E-431.3769912
ACATGGG47450.030.6562163
GAGTACT51850.029.60439312-13
CAGAGTA72850.029.359910-11
Produced by FastQC (version 0.11.5)