FastQCFastQC Report
Fri 27 May 2016
SRR940066_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940066_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803155
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22200.2764099084236542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9600.062.7962381
GTATCAA18350.056.5974241
ATCAACG22200.044.5949673
TCAACGC22250.044.2267154
CAACGCA22850.042.7696725
AACGCAG24050.040.635636
GTACATG50400.038.133691
TACATGG51000.036.5124322
TATCAAC27800.036.4972762
CGAATAC851.686718E-435.0817953
ACGCAGA28150.034.717127
CGCAGAG28050.034.4168
ACATGGG51950.033.292253
CATGGGA34100.029.382294
GAGTACA36900.028.4688421
TCTATAC3000.027.8315563
GCAGAGT34050.027.1179839
CGTATAG900.00938224726.5277821
TCGCGAA450.00955492526.43031568-69
AGTACAT38000.026.3865132
Produced by FastQC (version 0.11.5)