FastQCFastQC Report
Fri 27 May 2016
SRR940066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803155
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71970.896091040957225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51350.6393535494393984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32870.40926097702187003No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13540.16858514234487737No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.14081964253475357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.12488249466167801No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8790.10944338265963607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.079.22881
GTATCAA46400.060.514521
TCAACGC60150.046.267434
ATCAACG60700.045.84823
CAACGCA61550.045.249795
TATCAAC61900.045.151932
AACGCAG63450.044.1706666
TGGTATC8400.039.7285652
ACGCAGA73500.037.8834157
GTACATG51000.037.8731541
CGCAGAG74300.037.475528
GTGGTAT9200.036.9354741
TACATGG52250.036.1548962
ACATGGG52650.034.861533
GCAGAGT81750.033.987549
CATGGGA35850.031.9158334
GAGTACT58550.028.96066712-13
CAGAGTA78950.028.63664210-11
TAACGCA1254.8291877E-528.6045674
ATGGGAT17400.027.7034955
Produced by FastQC (version 0.11.5)