FastQCFastQC Report
Fri 27 May 2016
SRR940065_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940065_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206015
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17860.8669271654976579No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5030.24415697886076257No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4460.216489090600199No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3890.18882120233963548No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2610.1266898041404752No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.10727374220323763No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGC152.6776406E-4119.232423
GTATCAA4600.045.39331
GGTATCA2850.039.773181
GTACATG11400.037.156521
TATACAG1801.4551915E-1136.4144025
TACATGG11350.036.2777672
ATCAACG5050.035.415573
CAACGCA5250.034.049835
TCAACGC5300.033.7450264
TATCAAC5850.033.6624262
AACGCAG5350.032.2996066
ACATGGG11950.031.9283453
GGTCTAG750.0038521731.818541
CATGGGG4450.030.8128724
TGCTCCG800.005313907729.79365
GCCCGAT400.005370834429.73573538-39
GAACAAA3300.028.9259471
GAGTACA8150.028.5488451
GTTGTAG1055.7973445E-428.4094141
GTAGGCT1055.8221817E-428.388673
Produced by FastQC (version 0.11.5)