##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940065_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 206015 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.003456059024828 34.0 31.0 34.0 27.0 34.0 2 31.172322403708467 34.0 31.0 34.0 27.0 34.0 3 31.317501152828676 34.0 31.0 34.0 27.0 34.0 4 34.27401402810475 37.0 35.0 37.0 30.0 37.0 5 34.67985826274786 37.0 35.0 37.0 31.0 37.0 6 34.40066500012135 37.0 37.0 37.0 31.0 37.0 7 34.537489988593066 37.0 37.0 37.0 31.0 37.0 8 34.374652331140936 37.0 37.0 37.0 31.0 37.0 9 35.91458874353809 39.0 38.0 39.0 30.0 39.0 10-11 36.05378249156615 39.0 38.5 39.0 31.0 39.0 12-13 36.04039997087591 39.0 38.5 39.0 31.0 39.0 14-15 37.52235759532073 41.0 39.0 41.0 31.0 41.0 16-17 37.61550372545689 41.0 39.0 41.0 31.0 41.0 18-19 37.61330242943475 41.0 39.0 41.0 31.0 41.0 20-21 37.62278960269883 41.0 39.0 41.0 31.0 41.0 22-23 37.62788631895736 41.0 39.0 41.0 30.5 41.0 24-25 37.68768050870082 41.0 39.0 41.0 30.0 41.0 26-27 37.710953085940346 41.0 39.0 41.0 30.0 41.0 28-29 37.6526223818654 41.0 39.0 41.0 30.0 41.0 30-31 37.59047642161978 41.0 39.0 41.0 30.0 41.0 32-33 37.54260126689804 41.0 39.0 41.0 30.0 41.0 34-35 37.454593112152025 41.0 39.0 41.0 30.0 41.0 36-37 37.314455258112275 41.0 38.0 41.0 30.0 41.0 38-39 37.21199184525398 41.0 38.0 41.0 30.0 41.0 40-41 37.133000024270075 41.0 38.0 41.0 30.0 41.0 42-43 36.974358663204136 40.5 38.0 41.0 29.0 41.0 44-45 36.827529548819264 40.0 38.0 41.0 29.0 41.0 46-47 36.68951047253841 40.0 38.0 41.0 29.0 41.0 48-49 36.564944300172314 40.0 37.0 41.0 29.0 41.0 50-51 36.417180787806714 40.0 37.0 41.0 28.0 41.0 52-53 36.5520471810305 40.0 37.0 41.0 29.0 41.0 54-55 36.40046355847875 40.0 37.0 41.0 29.0 41.0 56-57 36.32660243186176 40.0 36.0 41.0 29.0 41.0 58-59 36.16575006674272 40.0 36.0 41.0 29.0 41.0 60-61 35.951149188165914 40.0 35.0 41.0 28.0 41.0 62-63 35.69935441594059 39.0 35.0 41.0 28.0 41.0 64-65 35.40897992864598 39.0 35.0 41.0 28.0 41.0 66-67 35.11443584205034 38.5 35.0 41.0 28.0 41.0 68-69 34.778999102007134 37.5 35.0 40.0 28.0 41.0 70-71 34.41840399970876 37.0 35.0 39.5 27.0 41.0 72-73 34.04824405989855 36.5 35.0 39.0 26.5 41.0 74-75 33.656476470159944 36.0 34.0 39.0 26.0 41.0 76-77 33.27959857291945 35.5 34.0 38.0 26.0 40.0 78-79 32.92317792393758 35.0 34.0 37.0 25.5 39.0 80-81 32.58602771642842 35.0 34.0 37.0 25.0 39.0 82-83 32.25233114093634 35.0 34.0 36.0 25.0 38.0 84-85 31.99823071135597 35.0 34.0 36.0 25.0 37.0 86-87 31.75799820401427 35.0 33.5 36.0 25.0 37.0 88-89 31.56420406281096 35.0 33.0 35.0 24.5 36.5 90-91 31.39429895881368 35.0 33.0 35.0 24.0 36.0 92-93 31.238475353736376 35.0 33.0 35.0 23.0 36.0 94-95 31.11385578719996 35.0 33.0 35.0 23.0 36.0 96-97 30.989233793655803 35.0 33.0 35.0 20.0 35.5 98-99 30.911479746620394 35.0 33.0 35.0 20.0 35.0 100-101 30.814671261801323 35.0 33.0 35.0 19.5 35.0 102-103 30.12949299808266 34.0 31.0 35.0 19.5 35.0 104-105 30.98104264252603 35.0 33.0 35.0 21.5 35.0 106-107 31.079673810159456 35.0 34.0 35.0 22.5 35.0 108-109 31.049986651457417 35.0 34.0 35.0 20.0 35.0 110-111 31.00162609518724 35.0 34.0 35.0 19.5 35.0 112-113 30.945615610513798 35.0 33.5 35.0 19.0 35.0 114-115 30.872128243089094 35.0 33.0 35.0 18.5 35.0 116-117 30.784836055627018 35.0 33.0 35.0 16.5 35.0 118-119 30.689430381282918 35.0 33.0 35.0 8.5 35.0 120-121 30.592233575225105 35.0 33.0 35.0 2.0 35.0 122-123 30.44196053685411 35.0 33.0 35.0 2.0 35.0 124-125 29.250333713564544 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.13586011999198888 1101 2 0.10772985404566882 1101 3 0.09055234726559647 1101 4 0.1603197024548848 1101 5 0.04697837202483868 1101 6 0.23333321263249474 1101 7 0.1294875468304184 1101 8 0.17299898955815252 1101 9 0.017073694668710004 1101 10-11 0.08600921347396451 1101 12-13 -0.06002153156122603 1101 14-15 0.05031885377451317 1101 16-17 0.08072507570229703 1101 18-19 0.26563232901315814 1101 20-21 0.21041685077334904 1101 22-23 0.28918961473826954 1101 24-25 0.19672276115004905 1101 26-27 0.3712175466956893 1101 28-29 0.2839138050221095 1101 30-31 0.36322433854945047 1101 32-33 0.35217646065440533 1101 34-35 0.303104007216092 1101 36-37 0.11969603622757319 1101 38-39 0.2130541310059897 1101 40-41 0.3234255139455513 1101 42-43 0.39934226571217835 1101 44-45 0.27754800235606325 1101 46-47 0.3568136409744085 1101 48-49 0.281956103701539 1101 50-51 0.2515344752298745 1101 52-53 0.39732607047541535 1101 54-55 0.5364235959636474 1101 56-57 0.6310485306619071 1101 58-59 0.638983967500387 1101 60-61 0.5826916987678317 1101 62-63 0.6167722228076187 1101 64-65 0.5277274060581405 1101 66-67 0.3188835733019033 1101 68-69 0.15135608212062124 1101 70-71 0.3216835778302496 1101 72-73 0.3209312406674627 1101 74-75 0.1941786472890712 1101 76-77 0.06392626418151792 1101 78-79 0.08544678919223969 1101 80-81 0.16575652163761134 1101 82-83 0.27176246879760413 1101 84-85 0.5063202067365111 1101 86-87 0.49522689764997097 1101 88-89 0.32524621491727146 1101 90-91 0.47757015507767164 1101 92-93 0.5885594222013282 1101 94-95 0.3607326713475061 1101 96-97 0.3875716972524401 1101 98-99 0.5189774038283979 1101 100-101 0.14416957652334972 1101 102-103 0.18274110182826675 1101 104-105 0.27739845495609927 1101 106-107 0.2809982941326439 1101 108-109 0.33724828313668453 1101 110-111 0.4844901027505166 1101 112-113 0.5244858358047217 1101 114-115 0.38037437499425764 1101 116-117 0.3980857086521503 1101 118-119 0.5567921625681969 1101 120-121 0.3364092254549824 1101 122-123 0.15588548229036192 1101 124-125 0.02837807823176064 1102 1 -0.258226924332412 1102 2 -0.15999379179228868 1102 3 -0.18047250802892023 1102 4 -0.09467282171203806 1102 5 0.38401261282068333 1102 6 -0.08571461789554746 1102 7 -0.05845933774568124 1102 8 -0.14023101443883235 1102 9 0.11569547672967673 1102 10-11 -0.0609538218623058 1102 12-13 0.005743594047572742 1102 14-15 0.11183533761635545 1102 16-17 -0.028873597892463465 1102 18-19 0.0354950993644465 1102 20-21 0.06692526663692178 1102 22-23 -0.09439464012102405 1102 24-25 0.06875278291138187 1102 26-27 0.2143656866196011 1102 28-29 0.07230331813072155 1102 30-31 -0.07038878338804722 1102 32-33 -0.14304196690403614 1102 34-35 0.007290473737683101 1102 36-37 -0.3444125632010113 1102 38-39 -0.038718603668556284 1102 40-41 -0.1379740212204723 1102 42-43 -0.35046158619230994 1102 44-45 -0.3152189440992146 1102 46-47 -0.09135885092060647 1102 48-49 -0.19164162246092076 1102 50-51 -0.20586807987663747 1102 52-53 -0.2828637120592674 1102 54-55 -0.1783104568260967 1102 56-57 -0.36842594047841715 1102 58-59 -0.4978533850184519 1102 60-61 -0.40865557958483123 1102 62-63 -0.4247667625515916 1102 64-65 -0.34857346291492775 1102 66-67 -0.6929815360228986 1102 68-69 -0.5053255362122044 1102 70-71 -0.32494637583438646 1102 72-73 -0.5197002821819368 1102 74-75 -0.29231304047276296 1102 76-77 -0.29896991796234573 1102 78-79 -0.34265198062612257 1102 80-81 -0.3753753639007229 1102 82-83 -0.26244871978583717 1102 84-85 -0.3641406302607173 1102 86-87 -0.3567664111093336 1102 88-89 -0.2570545652340215 1102 90-91 -0.1302224756753887 1102 92-93 0.037664542776894905 1102 94-95 -0.1209404348981451 1102 96-97 -0.14489266300869375 1102 98-99 -0.010563239537358271 1102 100-101 0.0631345067242357 1102 102-103 -0.2113611320394817 1102 104-105 -0.03609061256325674 1102 106-107 0.09455397798632248 1102 108-109 0.07215968341203549 1102 110-111 0.04838256918328554 1102 112-113 0.002961358002760761 1102 114-115 -0.007192056294694993 1102 116-117 -0.03600112944054956 1102 118-119 0.045008473207566624 1102 120-121 0.027849766675689835 1102 122-123 0.08123077695661252 1102 124-125 0.08880649527961992 1103 1 -0.7164701580261443 1103 2 -0.8690320223060333 1103 3 -0.6621063148270281 1103 4 -1.002212924884958 1103 5 -0.3056206286463521 1103 6 -0.5020385525998137 1103 7 -0.7034455578007979 1103 8 -0.7643565179368181 1103 9 -1.1554373054058615 1103 10-11 -0.8379448472315332 1103 12-13 -0.5253284149977233 1103 14-15 -0.6488458863254181 1103 16-17 -0.8125779297418134 1103 18-19 -0.7288042434381836 1103 20-21 -0.8426146952032312 1103 22-23 -0.7879003994313152 1103 24-25 -0.6361655974150935 1103 26-27 -0.6817617955371418 1103 28-29 -0.6462748742231739 1103 30-31 -0.6751304977545232 1103 32-33 -0.9318342784014533 1103 34-35 -0.892525793663907 1103 36-37 -0.7989034119042842 1103 38-39 -0.901145287295158 1103 40-41 -1.0526726290927044 1103 42-43 -0.9376851039649097 1103 44-45 -1.3562054468198639 1103 46-47 -1.3129827644249588 1103 48-49 -1.6319002614263596 1103 50-51 -1.5057479470238349 1103 52-53 -1.2483149169211103 1103 54-55 -1.0970017153943985 1103 56-57 -1.2474733576220771 1103 58-59 -1.3206998269660133 1103 60-61 -1.3268168402731177 1103 62-63 -1.3695056502627025 1103 64-65 -1.3891642338851753 1103 66-67 -1.2611745965839916 1103 68-69 -1.3518805499921456 1103 70-71 -1.3558911853884794 1103 72-73 -1.379441643216495 1103 74-75 -1.5041284598777551 1103 76-77 -1.3966449939287067 1103 78-79 -1.3624315015080413 1103 80-81 -1.254401581129187 1103 82-83 -1.08851048220545 1103 84-85 -1.1400292600395332 1103 86-87 -1.1494595070036198 1103 88-89 -1.12588362325112 1103 90-91 -1.3616124834486953 1103 92-93 -1.2578198752854348 1103 94-95 -1.18813003280642 1103 96-97 -1.2453166613126996 1103 98-99 -1.1898226558330265 1103 100-101 -1.2541151576104426 1103 102-103 -1.0921116090375698 1103 104-105 -1.2903694621145227 1103 106-107 -1.4091277406201357 1103 108-109 -1.4073187986740372 1103 110-111 -1.46006206370485 1103 112-113 -1.5949115838388046 1103 114-115 -1.7017719446418091 1103 116-117 -1.7032119272640287 1103 118-119 -1.5957024306154857 1103 120-121 -1.6400394284341964 1103 122-123 -1.8177516271137684 1103 124-125 -2.4113489707651823 1104 1 0.280840620546833 1104 2 0.2894874600911308 1104 3 0.04213716091937769 1104 4 0.13714946938960537 1104 5 -0.11889323762488146 1104 6 0.15191937409721845 1104 7 0.3025979613201457 1104 8 0.13111084272247808 1104 9 -0.005187901805776107 1104 10-11 0.17005052615095195 1104 12-13 0.08734417952262419 1104 14-15 0.16071819955617173 1104 16-17 0.0016055445587781492 1104 18-19 0.061863001555671815 1104 20-21 -0.3471907090830868 1104 22-23 -0.11101179970602715 1104 24-25 -0.26998327976888703 1104 26-27 0.08439883483950439 1104 28-29 0.04061878586502132 1104 30-31 0.16229450043820748 1104 32-33 0.11906574328580888 1104 34-35 0.3009535672845445 1104 36-37 0.08539500492504004 1104 38-39 0.28262540597455654 1104 40-41 0.328748609973303 1104 42-43 0.2823250167608933 1104 44-45 0.226603777498795 1104 46-47 0.19526562710255746 1104 48-49 0.20871999448342393 1104 50-51 0.25618366578611074 1104 52-53 0.379842388074195 1104 54-55 0.25134643757314734 1104 56-57 0.26436066247478607 1104 58-59 0.1586105178615682 1104 60-61 0.10690686391252768 1104 62-63 -0.022726209794264207 1104 64-65 0.2270216983059612 1104 66-67 0.4249744672488731 1104 68-69 0.48696857890252687 1104 70-71 0.3643213498533342 1104 72-73 0.41710285183157225 1104 74-75 0.4322338621059032 1104 76-77 0.4590696016037157 1104 78-79 0.3518893254998403 1104 80-81 0.2287401813229195 1104 82-83 0.37081142916590437 1104 84-85 0.35681162769270003 1104 86-87 0.36472396729696754 1104 88-89 0.3972783806525868 1104 90-91 0.25309148532164016 1104 92-93 0.202759897721144 1104 94-95 0.31999546419350366 1104 96-97 0.5319767674446183 1104 98-99 0.29973853841261544 1104 100-101 0.5316652226396847 1104 102-103 0.4239008249969842 1104 104-105 0.3886155736694725 1104 106-107 0.16379931786320157 1104 108-109 0.17872905790082072 1104 110-111 0.1107830168995001 1104 112-113 0.11097289530527021 1104 114-115 0.3733021535292309 1104 116-117 0.3435675132044196 1104 118-119 0.35586989642853695 1104 120-121 0.31148229498082713 1104 122-123 0.3008054465506582 1104 124-125 -0.24691609286450955 1105 1 0.053546994632515776 1105 2 0.2784808421879639 1105 3 0.21418871090196134 1105 4 0.3307544850635864 1105 5 0.11132619811179723 1105 6 0.3190249122372393 1105 7 0.3585784559213252 1105 8 0.4218527444988638 1105 9 0.36078910968847566 1105 10-11 0.28853885774273635 1105 12-13 0.2836938834585325 1105 14-15 0.4784611462646353 1105 16-17 0.3428594630540758 1105 18-19 0.3524307479854869 1105 20-21 0.3691520286389647 1105 22-23 0.3442093775841144 1105 24-25 0.158619994351632 1105 26-27 0.14671161784193743 1105 28-29 0.18573198684029535 1105 30-31 0.3097065519881852 1105 32-33 0.3992120337769336 1105 34-35 0.3847640862674737 1105 36-37 0.31289761725523846 1105 38-39 0.2619078859466981 1105 40-41 0.13140970366887217 1105 42-43 0.19847270049478283 1105 44-45 0.291856302751313 1105 46-47 0.09926423385978467 1105 48-49 0.007173495005879715 1105 50-51 0.2663961353089235 1105 52-53 0.2276027503173097 1105 54-55 0.27009948529170913 1105 56-57 0.6017204674208045 1105 58-59 0.4720274457612561 1105 60-61 0.4958932797954958 1105 62-63 0.5294994955348997 1105 64-65 0.3533400542794922 1105 66-67 0.3044171701398497 1105 68-69 0.3395774259941291 1105 70-71 0.3943317991741324 1105 72-73 0.3105755094421667 1105 74-75 0.2986845761428256 1105 76-77 0.14297764758072873 1105 78-79 0.2117708998641774 1105 80-81 0.17287114614354238 1105 82-83 0.26346207354069406 1105 84-85 0.12292099725034689 1105 86-87 0.1005233403377197 1105 88-89 0.09552289475917064 1105 90-91 0.24531719065079471 1105 92-93 0.22207720876259174 1105 94-95 0.3216812879482944 1105 96-97 0.17310529409038722 1105 98-99 0.013127601456854876 1105 100-101 0.1932604856142568 1105 102-103 0.11807707020771119 1105 104-105 0.32222058934344844 1105 106-107 0.3651089660693998 1105 108-109 0.3366949234528924 1105 110-111 0.4336600632248242 1105 112-113 0.34567160655570817 1105 114-115 0.5319158769705403 1105 116-117 0.6648841276244823 1105 118-119 0.7273850479436916 1105 120-121 0.704077209644705 1105 122-123 0.506399316282458 1105 124-125 0.3039512007614036 1106 1 -0.5484611464801006 1106 2 -0.22735362185545682 1106 3 -0.15876922668284266 1106 4 -0.3791912408522933 1106 5 -0.3179411017525169 1106 6 -0.2511514785361655 1106 7 -0.5940538371587252 1106 8 -0.08539284573180339 1106 9 -0.2838377559831642 1106 10-11 -0.5182250499913224 1106 12-13 -0.6757226701371266 1106 14-15 -0.22849950502437366 1106 16-17 -0.37834813531770095 1106 18-19 -0.2732001882424626 1106 20-21 -0.12238799849807691 1106 22-23 -0.03883675538738629 1106 24-25 -0.08366630144212905 1106 26-27 -0.2707578529857386 1106 28-29 -0.12653395617192587 1106 30-31 -0.15479819699417874 1106 32-33 -0.053433827824150626 1106 34-35 0.010930979076164249 1106 36-37 -0.02176316971898018 1106 38-39 0.02759309625876938 1106 40-41 0.10809898453725708 1106 42-43 0.1712067574825653 1106 44-45 0.23489565146230973 1106 46-47 0.25515297198732867 1106 48-49 0.08665789120669842 1106 50-51 0.24090088429128542 1106 52-53 0.10024182765788225 1106 54-55 0.30201264675711315 1106 56-57 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-1.1034290380534415 2216 60-61 -1.183525212160312 2216 62-63 -0.943026187737054 2216 64-65 -0.8882067571180698 2216 66-67 -0.8407698816143636 2216 68-69 -1.0947819459771821 2216 70-71 -1.0632452506417351 2216 72-73 -1.140217279858085 2216 74-75 -1.1222923573714212 2216 76-77 -1.1533976189437922 2216 78-79 -1.026236465904386 2216 80-81 -1.168260856182492 2216 82-83 -1.2410349220010843 2216 84-85 -1.035443026785316 2216 86-87 -1.1031828042639482 2216 88-89 -1.0647097084585084 2216 90-91 -1.1362916482714809 2216 92-93 -0.9300530490393086 2216 94-95 -0.901687581987737 2216 96-97 -0.9810369815444275 2216 98-99 -1.0918462306811563 2216 100-101 -1.2083212162659542 2216 102-103 -1.1968909468498588 2216 104-105 -0.9662112772973721 2216 106-107 -0.9384072192378312 2216 108-109 -1.0040842973263295 2216 110-111 -0.884409330068447 2216 112-113 -0.8582981550259952 2216 114-115 -0.807979451581506 2216 116-117 -0.6428305457618322 2216 118-119 -0.9707114823916427 2216 120-121 -1.0381325209232308 2216 122-123 -1.0440058009883337 2216 124-125 -0.944965255916248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4599.0 3 920.0 4 880.0 5 605.0 6 669.0 7 602.0 8 491.0 9 467.0 10 567.0 11 606.0 12 698.0 13 711.0 14 668.0 15 623.0 16 622.0 17 587.0 18 515.0 19 479.0 20 432.0 21 400.0 22 440.0 23 794.0 24 1956.0 25 1642.0 26 2613.0 27 1845.0 28 1648.0 29 1881.0 30 2044.0 31 2404.0 32 3149.0 33 4276.0 34 6257.0 35 11252.0 36 25702.0 37 74122.0 38 47481.0 39 368.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.13882480925855 19.804571966955955 11.453228884884924 23.603374338900572 2 14.8365094114728 22.054809848842357 39.72619400915606 23.382486730528782 3 19.719561280424152 27.000480668858 26.871816782626006 26.40814126809184 4 11.847118281660025 17.775779959179708 36.280007775294 34.097093983866266 5 12.807154683122695 40.80198870670946 33.03117550239606 13.359681107771785 6 31.01770470150631 38.26711470665359 16.901531558654714 13.81364903318539 7 27.887248713217446 35.01068272312324 19.295425852189958 17.806642711469358 8 25.240029542097485 38.84202752079608 18.494421985539923 17.42352095156651 9 25.22891636533302 19.64889770208795 18.67150744585715 36.45067848672188 10-11 24.275079035931604 30.080759910450226 26.006099485720114 19.638061567898056 12-13 24.226754066521 27.441612041757708 26.407137654770573 21.924496236950716 14-15 21.412117107604324 28.557418991511373 24.299026854353425 25.731437046530875 16-17 21.524488393717725 31.88650340261575 24.999210952465347 21.589797251201173 18-19 21.344666383340417 30.945297796126525 26.4870378240544 21.22299799647866 20-21 22.39141324488496 30.102276540592065 26.507764360297443 20.998545854225533 22-23 21.90252424259359 30.265884942743497 26.048366130588068 21.783224684074845 24-25 21.52339917031798 30.088845925911535 26.510477892957418 21.877277010813067 26-27 21.423144423865363 30.33491034345484 26.338172788200826 21.903772444478967 28-29 21.37558920188933 30.455487647998293 26.552798799994175 21.616124350118206 30-31 21.62895576149167 30.224712010369082 26.575387020199326 21.57094520793992 32-33 21.313990150413716 30.25604555372976 26.559173974567774 21.870790321288748 34-35 21.622093954648943 30.46826833921541 26.418616166568764 21.49102153956688 36-37 21.805106199136425 30.294726571506935 26.19190357492191 21.708263654434734 38-39 21.304070274062045 30.404600145150212 26.611762021617185 21.679567559170557 40-41 21.92068427350552 30.11415076250185 26.442297190041725 21.522867773950907 42-43 21.555329545095965 30.11236064149212 26.365549533105003 21.96676028030691 44-45 21.6515890373053 30.415142519400263 26.467125751555297 21.466142691739144 46-47 21.544795376149107 30.46964323426815 26.32449004857374 21.661071341009002 48-49 21.48329400798159 30.276879606163888 26.646081544272576 21.593744841581948 50-51 21.68444252418795 30.344286886320276 26.513779728240554 21.45749086125122 52-53 21.551311896028995 30.419627100623604 26.546202189042166 21.482858814305235 54-55 21.610193148413813 30.36631930537451 26.506263912050354 21.51722363416132 56-57 21.447401639025905 30.493358449528102 26.611838272420087 21.447401639025905 58-59 21.59955721599557 30.309584671516898 26.579420739478415 21.51143737300911 60-61 21.443412576277606 30.361035190857855 26.576889281570555 21.618662951293988 62-63 21.347976384681118 30.499883477044975 26.621125611745516 21.53101452652839 64-65 21.310388863127073 30.33797612486738 26.673189396275248 21.6784456157303 66-67 21.197672244894985 30.654844998409803 26.288968897715215 21.858513858979993 68-69 21.51790245003897 30.43307249114407 26.547632632547568 21.50139242626939 70-71 21.205809092365733 30.214903400290353 26.682058527916563 21.897228979427343 72-73 21.301474943439462 30.237797003505296 26.714779535281153 21.74594851777409 74-75 21.45596566773631 30.30312592542248 26.535897896469194 21.705010510372013 76-77 21.331443246418463 30.364052100375265 26.60580522071781 21.698699432488457 78-79 21.36482812196543 30.187415032045056 26.61074966012818 21.83700718586133 80-81 21.135102987049244 30.321038002645984 26.685358482321668 21.858500527983104 82-83 21.45246568625071 30.276209295663868 26.668107026853814 21.603217991231606 84-85 21.523519616267837 30.035149555532897 26.621176150735277 21.82015467746399 86-87 21.270614283182532 30.18127702343467 26.787097212747412 21.761011480635382 88-89 21.570212621326228 30.00733176986983 26.71264803134696 21.709807577456992 90-91 21.390300331867806 30.238570953434113 26.77592975181532 21.59519896288276 92-93 21.39446013105536 30.136663696596393 26.80443908159277 21.664437090755474 94-95 21.231498272727052 30.245362581660075 26.73522350159375 21.787915644019122 96-97 21.489890652009244 30.032338260143337 26.678336279060733 21.799434808786682 98-99 21.138756166724935 30.127607504952802 26.56668803169794 22.166948296624327 100-101 20.949664388799206 30.251131853326374 26.897932926311192 21.90127083156323 102-103 21.156810903855632 30.228871230495447 26.764847981626204 21.849469884022714 104-105 20.84279114481801 30.326740581233597 26.851283146847543 21.97918512710085 106-107 20.756998227682146 30.49212168297361 26.71757022506009 22.033309864284153 108-109 20.83932143481385 30.573789594298034 26.547115029811035 22.039773941077083 110-111 20.63946360990341 30.795578957078597 26.559757634018627 22.005199798999367 112-113 20.80391737960904 30.762694582285366 26.57510900492343 21.858279033182168 114-115 20.843508022428818 30.799087312182927 26.519892225162028 21.83751244022623 116-117 20.593868104287374 30.716763314456873 26.79348604412382 21.89588253713193 118-119 20.7535076368033 30.632916309570348 26.59321610053243 22.020359953093934 120-121 20.50238154195738 30.76947353599503 26.803878441825802 21.924266480221792 122-123 20.596974346027615 30.74471287359447 26.583467655797875 22.07484512458004 124-125 20.7053003121829 30.662138477149476 26.49136520544354 22.14119600522409 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1656.0 1 1665.5 2 1575.0 3 1162.5 4 622.5 5 321.0 6 206.0 7 164.5 8 194.5 9 206.0 10 144.0 11 95.5 12 87.5 13 86.0 14 87.5 15 85.0 16 91.0 17 103.0 18 114.0 19 143.0 20 190.5 21 287.5 22 375.0 23 462.5 24 611.0 25 815.5 26 1168.0 27 1468.5 28 1687.0 29 2097.0 30 2481.0 31 2811.5 32 3247.0 33 3659.5 34 4074.0 35 4537.0 36 5100.0 37 5568.0 38 5901.5 39 6258.5 40 6632.5 41 6868.5 42 7037.0 43 7330.5 44 7553.0 45 7714.5 46 7769.0 47 7676.5 48 7708.0 49 7666.5 50 7405.5 51 7085.5 52 6837.5 53 6571.5 54 6309.0 55 5929.0 56 5268.5 57 4618.5 58 4129.5 59 3669.5 60 3095.0 61 2457.0 62 1908.5 63 1518.0 64 1101.5 65 780.5 66 596.5 67 455.5 68 355.0 69 265.0 70 188.5 71 133.5 72 119.5 73 116.0 74 97.5 75 74.0 76 53.5 77 36.0 78 19.5 79 15.5 80 14.5 81 8.0 82 4.5 83 6.0 84 6.0 85 4.5 86 4.5 87 1.5 88 1.5 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.5 95 1.0 96 1.5 97 1.0 98 0.5 99 0.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05242336723054146 2 0.2271679246656797 3 0.025240880518408854 4 0.11406936388127077 5 0.025240880518408854 6 0.0393175254229061 7 0.03640511613232046 8 0.10047812052520447 9 0.1276606072373371 10-11 0.04659854864937019 12-13 0.03155110064801107 14-15 0.016260951872436473 16-17 0.03422080916438123 18-19 0.06188869742494479 20-21 0.19221901317865203 22-23 0.11188505691333156 24-25 0.07426643690993374 26-27 0.016746353420867412 28-29 0.007766424774895032 30-31 0.008737227871756912 32-33 0.0080091255491105 34-35 0.010678834065480668 36-37 0.005096716258524864 38-39 0.0104361332912652 40-41 0.007523724000679562 42-43 0.012863141033419897 44-45 0.012377739484988956 46-47 0.01917336116302211 48-49 0.01990146348566852 50-51 0.011649637162342548 52-53 0.016746353420867412 54-55 0.016260951872436473 56-57 0.01893066038880664 58-59 0.022085770453607746 60-61 0.011649637162342548 62-63 0.022813872776254157 64-65 0.03325000606751936 66-67 0.031793801422226535 68-69 0.03858942310025969 70-71 0.03009489600271825 72-73 0.01990146348566852 74-75 0.013591243356066306 76-77 0.013591243356066306 78-79 0.026697085163701673 80-81 0.020629565808314928 82-83 0.023299274324685097 84-85 0.018445258840375702 86-87 0.0288813921316409 88-89 0.03009489600271825 90-91 0.0288813921316409 92-93 0.03470621071281217 94-95 0.026454384389486205 96-97 0.032521903744872946 98-99 0.034463509938596704 100-101 0.023056573550469625 102-103 0.0337354076159503 104-105 0.02742518748634808 106-107 0.03397810839016576 108-109 0.02475547896997791 110-111 0.022085770453607746 112-113 0.029609494454287313 114-115 0.014562046452928185 116-117 0.012863141033419897 118-119 0.03470621071281217 120-121 0.027182486712132612 122-123 0.0208722665825304 124-125 0.022328471227823218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 206015.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.88684493365561 #Duplication Level Percentage of deduplicated Percentage of total 1 83.39374192700497 62.45094220124915 2 11.011842460431717 16.49284277536389 3 2.96467080820952 6.660445292811653 4 1.120666438061877 3.3569269507796777 5 0.5472296807986687 2.0490152124531877 6 0.29184024324167984 1.3112997024624007 7 0.18261561180136332 0.957285490240334 8 0.11621806106338554 0.6962563133875097 9 0.0804783399594044 0.5424092068552144 >10 0.2796658442528246 3.307161151365792 >50 0.005190863611587121 0.25218689968130126 >100 0.00454200566013873 0.8109825348132791 >500 6.488579514483901E-4 0.24441235171424766 >1k 6.488579514483901E-4 0.8678339168223584 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1786 0.8669271654976579 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 503 0.24415697886076257 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 446 0.216489090600199 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 389 0.18882120233963548 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 261 0.1266898041404752 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 221 0.10727374220323763 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.854015484309395E-4 0.0 0.0 0.0 0.0 7 4.854015484309395E-4 0.0 0.0 0.0 0.0 8 4.854015484309395E-4 0.0 0.0 0.0 0.0 9 4.854015484309395E-4 0.0 0.0 0.0 0.0 10-11 4.854015484309395E-4 0.0 0.0 0.0 0.0 12-13 4.854015484309395E-4 0.0 0.0 0.0 0.0 14-15 9.70803096861879E-4 0.0 0.0 0.0 0.0 16-17 9.70803096861879E-4 0.0 0.0 0.0 0.0 18-19 9.70803096861879E-4 0.0 0.0 2.4270077421546975E-4 0.0 20-21 9.70803096861879E-4 0.0 0.0 4.854015484309395E-4 0.0 22-23 9.70803096861879E-4 0.0 0.0 4.854015484309395E-4 0.0 24-25 0.001941606193723758 0.0 0.0 9.70803096861879E-4 0.0 26-27 0.001941606193723758 0.0 0.0 9.70803096861879E-4 0.0 28-29 0.002912409290585637 0.0 0.0 0.0012135038710773488 0.0 30-31 0.002912409290585637 0.0 0.0 0.0036405116132320462 0.0 32-33 0.002912409290585637 0.0 0.0 0.008737227871756912 0.0 34-35 0.002912409290585637 0.0 0.0 0.014804747227143655 0.0 36-37 0.002912409290585637 0.0 0.0 0.026697085163701673 0.0 38-39 0.002912409290585637 0.0 0.0 0.058005485037497265 0.0 40-41 0.002912409290585637 0.0 0.0 0.08688687716913818 0.0 42-43 0.0033978108390165765 0.0 0.0 0.12062228478508846 0.0 44-45 0.003883212387447516 0.0 0.0 0.15071718078780671 0.0 46-47 0.003883212387447516 0.0 0.0 0.18639419459748077 0.0 48-49 0.003883212387447516 0.0 0.0 0.22279931072980125 0.0 50-51 0.003883212387447516 0.0 0.0 0.26721355241123224 0.0 52-53 0.003883212387447516 0.0 0.0 0.3123558964153096 0.0 54-55 0.003883212387447516 0.0 0.0 0.3596825473873262 0.0 56-57 0.003883212387447516 0.0 0.0 0.4201150401669781 0.0 58-59 0.003883212387447516 0.0 0.0 0.4883139577215251 0.0 60-61 0.003883212387447516 0.0 0.0 0.5696187170837075 0.0 62-63 0.003883212387447516 0.0 0.0 0.6361187292187462 0.0 64-65 0.003883212387447516 0.0 0.0 0.7065019537412325 0.0 66-67 0.004368613935878456 0.0 0.0 0.8045530665242822 0.0 68-69 0.004854015484309395 0.0 0.0 0.9106133048564424 0.0 70-71 0.005096716258524865 0.0 0.0 1.026624274931437 0.0 72-73 0.005339417032740335 0.0 0.0 1.1618086061694537 0.0 74-75 0.005339417032740335 0.0 0.0 1.284372497148266 0.0 76-77 0.005339417032740335 0.0 0.0 1.4205276314831443 0.0 78-79 0.005339417032740335 0.0 0.0 1.584593354852802 0.0 80-81 0.005339417032740335 0.0 0.0 1.7734145571924373 0.0 82-83 0.005339417032740335 0.0 0.0 1.9678178773390287 0.0 84-85 0.005824818581171274 0.0 0.0 2.163192000582482 0.0 86-87 0.006310220129602214 0.0 0.0 2.354925612212703 0.0 88-89 0.006310220129602214 0.0 0.0 2.576026017522996 0.0 90-91 0.006795621678033153 0.0 0.0 2.8264932165133607 0.0 92-93 0.006795621678033153 0.0 0.0 3.111423925442322 0.0 94-95 0.006795621678033153 0.0 0.0 3.391015217338543 0.0 96-97 0.006795621678033153 0.0 0.0 3.662111982137223 0.0 98-99 0.006795621678033153 0.0 0.0 3.9776229886173335 0.0 100-101 0.007038322452248623 0.0 0.0 4.34749896852171 0.0 102-103 0.0072810232264640925 0.0 0.0 4.7144625391355 0.0 104-105 0.008251826323325972 0.0 0.0 5.086765526782031 0.0 106-107 0.008251826323325972 0.0 0.0 5.448632381137296 0.0 108-109 0.008251826323325972 0.0 0.0 5.843021139237434 0.0 110-111 0.008251826323325972 0.0 0.0 6.285950052180667 0.0 112-113 0.008251826323325972 0.0 0.0 6.764798679707789 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGCGC 15 2.6776406E-4 119.23242 3 GTATCAA 460 0.0 45.3933 1 GGTATCA 285 0.0 39.77318 1 GTACATG 1140 0.0 37.15652 1 TATACAG 180 1.4551915E-11 36.414402 5 TACATGG 1135 0.0 36.277767 2 ATCAACG 505 0.0 35.41557 3 CAACGCA 525 0.0 34.04983 5 TCAACGC 530 0.0 33.745026 4 TATCAAC 585 0.0 33.662426 2 AACGCAG 535 0.0 32.299606 6 ACATGGG 1195 0.0 31.928345 3 GGTCTAG 75 0.00385217 31.81854 1 CATGGGG 445 0.0 30.812872 4 TGCTCCG 80 0.0053139077 29.7936 5 GCCCGAT 40 0.0053708344 29.735735 38-39 GAACAAA 330 0.0 28.925947 1 GAGTACA 815 0.0 28.548845 1 GTTGTAG 105 5.7973445E-4 28.409414 1 GTAGGCT 105 5.8221817E-4 28.38867 3 >>END_MODULE