FastQCFastQC Report
Fri 27 May 2016
SRR940065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206015
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24761.2018542339150062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.8955658568550833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.5693760163094921No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760.3281314467393151No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.3174526126738344No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5640.27376647331504983No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.20435405188942551No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2920.14173725214183433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6650.075.3164441
GAATACG300.00419644759.625524
GGGCGAC150.004235959659.4952710-11
GTATCAA11900.052.1096951
ATCAACG13850.044.772953
TCTAATA1204.0563464E-1044.719142
TCAACGC13900.044.6118974
TATCAAC14000.044.293242
CAACGCA14150.043.7598345
AACGCAG14550.042.934736
ACGCAGA17350.036.0057837
GAGTTAG851.6846131E-435.0738331
CGCAGAG17900.034.899468
GTACATG9550.034.3393021
ACATGGG9250.034.1638073
TACATGG9350.033.798422
GTATAAG902.3568553E-433.1252861
GTGGTAT2400.032.2971571
GCAGAGT19200.032.2266049
GTGTAGC1901.0404619E-931.3818511
Produced by FastQC (version 0.11.5)