##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940065_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 206015 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.1093900929544 34.0 33.0 34.0 31.0 34.0 2 33.14617867630998 34.0 33.0 34.0 31.0 34.0 3 33.210246826687374 34.0 33.0 34.0 31.0 34.0 4 36.4364439482562 37.0 37.0 37.0 35.0 37.0 5 36.45610271096765 37.0 37.0 37.0 35.0 37.0 6 36.54787758172949 37.0 37.0 37.0 35.0 37.0 7 36.535994951823895 37.0 37.0 37.0 35.0 37.0 8 36.54158192364633 37.0 37.0 37.0 35.0 37.0 9 38.39903890493411 39.0 39.0 39.0 37.0 39.0 10-11 38.39214862995413 39.0 39.0 39.0 37.0 39.0 12-13 38.37552605392811 39.0 39.0 39.0 37.0 39.0 14-15 39.95952721889183 41.0 40.0 41.0 38.0 41.0 16-17 39.97521296992937 41.0 40.0 41.0 38.0 41.0 18-19 40.035160061160596 41.0 40.0 41.0 38.0 41.0 20-21 40.029497852098146 41.0 40.0 41.0 38.0 41.0 22-23 39.97363056088149 41.0 40.0 41.0 38.0 41.0 24-25 39.94427590224013 41.0 40.0 41.0 38.0 41.0 26-27 39.839596631313256 41.0 40.0 41.0 37.0 41.0 28-29 39.74362061014975 41.0 40.0 41.0 37.0 41.0 30-31 39.548532873819866 41.0 39.5 41.0 36.5 41.0 32-33 39.32039900007281 41.0 39.0 41.0 35.0 41.0 34-35 39.18347693129141 41.0 39.0 41.0 35.0 41.0 36-37 39.07091473921802 41.0 39.0 41.0 35.0 41.0 38-39 38.9474407203359 41.0 39.0 41.0 35.0 41.0 40-41 38.81137295827973 41.0 39.0 41.0 35.0 41.0 42-43 38.63804334635827 41.0 39.0 41.0 35.0 41.0 44-45 38.41213503871077 41.0 38.0 41.0 35.0 41.0 46-47 38.08861733368929 41.0 38.0 41.0 34.5 41.0 48-49 37.787547023275 41.0 38.0 41.0 33.5 41.0 50-51 37.81381452806835 41.0 38.0 41.0 34.0 41.0 52-53 37.75583331310827 41.0 38.0 41.0 34.0 41.0 54-55 37.62860956726452 41.0 37.5 41.0 34.0 41.0 56-57 37.47485377278353 41.0 37.0 41.0 34.0 41.0 58-59 37.29523578380215 40.0 37.0 41.0 33.5 41.0 60-61 37.07865689391549 40.0 36.0 41.0 33.0 41.0 62-63 36.84579520908672 39.5 36.0 41.0 33.0 41.0 64-65 36.56852656359974 39.0 35.0 41.0 33.0 41.0 66-67 36.26764555978934 39.0 35.0 41.0 32.0 41.0 68-69 35.938014222265366 38.0 35.0 41.0 32.0 41.0 70-71 35.58959784481712 37.0 35.0 40.0 32.0 41.0 72-73 35.219930587578574 37.0 35.0 39.0 31.0 41.0 74-75 34.851627308691114 36.0 35.0 39.0 31.0 41.0 76-77 34.47731475863408 36.0 35.0 38.5 31.0 40.5 78-79 34.13465038953474 35.0 35.0 37.0 31.0 39.0 80-81 33.813360677620565 35.0 35.0 37.0 30.5 39.0 82-83 33.51355726524768 35.0 35.0 36.5 30.0 39.0 84-85 33.25396209013907 35.0 35.0 36.0 30.0 37.0 86-87 33.03132053491251 35.0 35.0 36.0 30.0 37.0 88-89 32.906004417154094 35.0 35.0 35.5 30.0 37.0 90-91 32.869300779069484 35.0 35.0 35.0 30.0 36.0 92-93 32.76402446423804 35.0 35.0 35.0 30.0 36.0 94-95 32.673346600975655 35.0 35.0 35.0 30.0 36.0 96-97 32.596791495764876 35.0 35.0 35.0 30.0 36.0 98-99 32.53303400237847 35.0 35.0 35.0 30.0 36.0 100-101 32.4718175860981 35.0 35.0 35.0 30.0 35.0 102-103 31.991289469213406 35.0 33.5 35.0 28.0 35.0 104-105 32.3563478387496 35.0 34.5 35.0 29.0 35.0 106-107 32.388144067179574 35.0 35.0 35.0 29.5 35.0 108-109 32.365405431643325 35.0 35.0 35.0 29.5 35.0 110-111 32.31694536805573 35.0 35.0 35.0 29.0 35.0 112-113 32.27371550615247 35.0 35.0 35.0 29.0 35.0 114-115 32.205965585030214 35.0 35.0 35.0 29.0 35.0 116-117 32.140016503652646 35.0 35.0 35.0 29.0 35.0 118-119 32.07666189355144 35.0 35.0 35.0 29.0 35.0 120-121 31.9895274615926 35.0 35.0 35.0 29.0 35.0 122-123 31.88696696842463 35.0 35.0 35.0 27.0 35.0 124-125 30.88182171201126 34.5 32.5 35.0 22.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11015367099365392 1101 2 0.1061673216499841 1101 3 0.20331044818294686 1101 4 0.2040796086645642 1101 5 0.13620561494915506 1101 6 0.18928809476638264 1101 7 0.14566637482007394 1101 8 0.19558950320763557 1101 9 0.22909101370775886 1101 10-11 0.1871447394143928 1101 12-13 0.31562179566481774 1101 14-15 0.20355128186024984 1101 16-17 0.26776513457343043 1101 18-19 0.30503122970507945 1101 20-21 0.39185333983294157 1101 22-23 0.24387537456520647 1101 24-25 0.22778327219848649 1101 26-27 0.12762292907870432 1101 28-29 0.07184652152368898 1101 30-31 -0.004682513649079567 1101 32-33 -0.0015909174115691371 1101 34-35 0.010715807818669987 1101 36-37 0.2109793404999536 1101 38-39 0.03665418581580582 1101 40-41 -0.14114502267420193 1101 42-43 -0.14626899023452467 1101 44-45 0.06780731503358339 1101 46-47 0.08241380770948581 1101 48-49 0.2681505336029346 1101 50-51 0.17057367088153086 1101 52-53 0.07822071026432553 1101 54-55 0.06921505151667162 1101 56-57 -0.07738460751009768 1101 58-59 -0.05744378994249644 1101 60-61 0.14686858820719806 1101 62-63 0.14345594877950418 1101 64-65 0.29869099246212727 1101 66-67 0.20688974293872775 1101 68-69 0.2987716807781382 1101 70-71 0.33517785562551694 1101 72-73 0.19662515827857874 1101 74-75 -0.05460362859835044 1101 76-77 -0.1550627370142763 1101 78-79 -0.08287162897726574 1101 80-81 0.05112058860875379 1101 82-83 0.11355313107441845 1101 84-85 0.04199348228215172 1101 86-87 0.036574678112408776 1101 88-89 0.11395577698998238 1101 90-91 0.07612690943197009 1101 92-93 0.3424640112182473 1101 94-95 0.21770154839416023 1101 96-97 0.3627961939746598 1101 98-99 0.39125622131241755 1101 100-101 0.33682795168842006 1101 102-103 0.1808430528900189 1101 104-105 0.29005920709436595 1101 106-107 0.3813872822515165 1101 108-109 0.29205408252052223 1101 110-111 0.20027388035970972 1101 112-113 0.2898035405891477 1101 114-115 0.127381337262797 1101 116-117 0.1749804465823388 1101 118-119 0.11794078391601204 1101 120-121 0.058305422654449046 1101 122-123 -0.2157866969417377 1101 124-125 0.11348329085939923 1102 1 -0.015262681095876474 1102 2 0.03943255828600911 1102 3 0.05691735259591013 1102 4 0.06772337411585028 1102 5 0.0037871459405565133 1102 6 0.029853001281459512 1102 7 0.004735818647695567 1102 8 0.043437795608930685 1102 9 0.13892210429116858 1102 10-11 -0.06553841849592601 1102 12-13 0.043405248167374566 1102 14-15 0.0656406661772806 1102 16-17 0.0974123640741027 1102 18-19 0.07747723346519564 1102 20-21 0.06839402760958535 1102 22-23 0.1031668728405819 1102 24-25 0.05429213217094997 1102 26-27 -0.07483151275043554 1102 28-29 -0.03305199959368821 1102 30-31 0.08417627452577392 1102 32-33 0.16036079314785923 1102 34-35 0.17295618916017474 1102 36-37 0.04999726769570145 1102 38-39 0.1646241640544659 1102 40-41 0.3213425792858047 1102 42-43 0.1285011834120482 1102 44-45 0.04096833411546896 1102 46-47 -0.26003115979654723 1102 48-49 -0.13205819757794757 1102 50-51 0.02898435318083159 1102 52-53 0.31404253377544933 1102 54-55 0.22719198746136726 1102 56-57 0.1251586560981437 1102 58-59 0.1970824198841541 1102 60-61 0.2219303933618164 1102 62-63 0.15396652597303273 1102 64-65 0.16300832307159396 1102 66-67 -0.030419845372321674 1102 68-69 -0.14062966043072578 1102 70-71 -0.12145491072878656 1102 72-73 0.03431075878782508 1102 74-75 0.07279640989109026 1102 76-77 0.13723493437474588 1102 78-79 0.25837424451091806 1102 80-81 0.3233519397360496 1102 82-83 0.23883625049530366 1102 84-85 0.21225743100238503 1102 86-87 0.2991762680222436 1102 88-89 0.3408584135164574 1102 90-91 0.40736552914152213 1102 92-93 0.3594437746562349 1102 94-95 0.40879420431339497 1102 96-97 0.28694979643798746 1102 98-99 0.330694571595906 1102 100-101 0.19457247064446648 1102 102-103 0.2993831189142071 1102 104-105 0.3290075572298221 1102 106-107 0.3024839351508106 1102 108-109 0.17692625317675947 1102 110-111 0.30557209948304376 1102 112-113 0.187451243805981 1102 114-115 0.2514727644807522 1102 116-117 0.14280475710327778 1102 118-119 0.15378037178295756 1102 120-121 0.07268714905376328 1102 122-123 0.20769334036341647 1102 124-125 0.2527040277840946 1103 1 -0.044518564072348 1103 2 -0.015616554850872433 1103 3 0.02135802816654575 1103 4 -0.14332884834192328 1103 5 -0.1021417636771389 1103 6 0.04944172292657356 1103 7 -0.02509952867757903 1103 8 0.0065075433299384144 1103 9 0.11671872643801606 1103 10-11 0.09749599603308212 1103 12-13 0.07325472876944872 1103 14-15 0.13593251834321052 1103 16-17 0.12468958829853705 1103 18-19 0.14463149818146803 1103 20-21 0.15240222491012645 1103 22-23 0.00591579517822538 1103 24-25 0.03186262188899747 1103 26-27 0.1198967750030846 1103 28-29 -0.010389290244525284 1103 30-31 -0.01850904806627085 1103 32-33 -0.039625073575336955 1103 34-35 -0.10334937228424934 1103 36-37 -0.14691461132344585 1103 38-39 -0.27574047448698025 1103 40-41 -0.14712608017288886 1103 42-43 0.1007292944996081 1103 44-45 0.08991187167465142 1103 46-47 0.0729276417015825 1103 48-49 -0.5588760903063843 1103 50-51 -0.7096560255909807 1103 52-53 -0.4550479898624573 1103 54-55 -0.4943541930205768 1103 56-57 -0.6078566785071828 1103 58-59 -0.4009742545558126 1103 60-61 -0.48884623019834805 1103 62-63 -0.5760384206738465 1103 64-65 -0.555682935496705 1103 66-67 -0.5134175133816541 1103 68-69 -0.487439073193066 1103 70-71 -0.5047281776357195 1103 72-73 -0.41282371934092765 1103 74-75 -0.5920187975147471 1103 76-77 -0.628237470402297 1103 78-79 -0.5784641414688707 1103 80-81 -0.468224627291022 1103 82-83 -0.5914584283259856 1103 84-85 -0.49182708599288105 1103 86-87 -0.7285532210597907 1103 88-89 -0.5782285768022462 1103 90-91 -0.6717737543013413 1103 92-93 -0.7471847321630634 1103 94-95 -0.620772610752681 1103 96-97 -0.49111535796806294 1103 98-99 -0.6041722222702504 1103 100-101 -0.5078584529651664 1103 102-103 -0.6604501084195462 1103 104-105 -0.8277870155918876 1103 106-107 -0.6015793819292554 1103 108-109 -0.6413339404491296 1103 110-111 -0.6648540188124876 1103 112-113 -0.47675623269744705 1103 114-115 -0.4428103250535571 1103 116-117 -0.41884459629930504 1103 118-119 -0.36862793858379916 1103 120-121 -0.16593202980285326 1103 122-123 -0.22499692887551248 1103 124-125 -0.7323217654460663 1104 1 0.05271269451220206 1104 2 -0.009471828471923516 1104 3 0.10569259044515178 1104 4 -0.26096059850458175 1104 5 -0.09281623776085013 1104 6 -0.0790625783818939 1104 7 0.14374309403621766 1104 8 0.14933990073614467 1104 9 0.12493097251623198 1104 10-11 0.13412579434147176 1104 12-13 0.08068623314305512 1104 14-15 0.1149970741156352 1104 16-17 0.21881233824432655 1104 18-19 0.23221915089600031 1104 20-21 0.17868719563231394 1104 22-23 0.14027565927133878 1104 24-25 0.12007197633126765 1104 26-27 0.317156949947055 1104 28-29 0.047472344975595604 1104 30-31 0.14687806969560313 1104 32-33 0.13549962822470718 1104 34-35 -0.007744658917658853 1104 36-37 0.0038480576565262936 1104 38-39 -0.09817234031507382 1104 40-41 -0.2262918432033274 1104 42-43 -0.37946299929060245 1104 44-45 -0.15860977944947052 1104 46-47 0.08940273748702054 1104 48-49 -0.03366825397297646 1104 50-51 -0.14043749020459018 1104 52-53 -0.22016040220974276 1104 54-55 -0.010850976075595042 1104 56-57 0.1376215257868978 1104 58-59 0.04782933107275511 1104 60-61 -0.013960088363610623 1104 62-63 -0.06003321572914899 1104 64-65 -0.12764129593912799 1104 66-67 -0.1414663820290869 1104 68-69 0.03288189839660305 1104 70-71 0.013785521489559471 1104 72-73 -0.0939341075430562 1104 74-75 -0.17443765098109765 1104 76-77 -0.2767518924365646 1104 78-79 -0.21973501546583663 1104 80-81 -0.29850005561458914 1104 82-83 -0.26914945702547044 1104 84-85 -0.2101455996920123 1104 86-87 -0.41363960868358873 1104 88-89 -0.40885100143898967 1104 90-91 -0.14595144533004145 1104 92-93 -0.1588307959236417 1104 94-95 -0.1812656346809689 1104 96-97 -0.12324866238504484 1104 98-99 -0.12033646719181945 1104 100-101 -0.14999681774504126 1104 102-103 -0.013135715301643813 1104 104-105 -0.12368089255277681 1104 106-107 0.10045199232072122 1104 108-109 -0.04919196112432189 1104 110-111 -0.11392508076578167 1104 112-113 -0.19586271719104786 1104 114-115 -0.0682784086218362 1104 116-117 0.04889197566628667 1104 118-119 0.1276707674091142 1104 120-121 0.15620344334107727 1104 122-123 0.16367508731765312 1104 124-125 0.2925679909336054 1105 1 0.15205090419523515 1105 2 0.13525823074089516 1105 3 0.10307329403275389 1105 4 0.044870122498558374 1105 5 -0.07154142062396573 1105 6 -0.00565261669606798 1105 7 -2.6247893487152396E-4 1105 8 -0.13784923197023602 1105 9 0.06450603347060735 1105 10-11 0.12967438368316664 1105 12-13 0.09198543202845144 1105 14-15 0.3110611632831777 1105 16-17 0.18681651797263754 1105 18-19 0.15135534037700893 1105 20-21 0.2045806125602141 1105 22-23 0.17075284492093346 1105 24-25 0.15347497180322733 1105 26-27 0.20185217809056155 1105 28-29 0.2723208298240891 1105 30-31 0.1363451543351104 1105 32-33 0.005760861244553439 1105 34-35 0.2379885350913966 1105 36-37 0.18354851046043308 1105 38-39 0.2802114980687662 1105 40-41 0.4161672302902204 1105 42-43 0.27373100976547704 1105 44-45 0.14322233479643387 1105 46-47 -0.11252690375296481 1105 48-49 0.12403986166617642 1105 50-51 0.3397436313558373 1105 52-53 0.26217327943428614 1105 54-55 0.12115181040995537 1105 56-57 0.18878140039504387 1105 58-59 0.29686451045276385 1105 60-61 0.1471057423573967 1105 62-63 0.22962195668463892 1105 64-65 0.2059637197348536 1105 66-67 0.2676802567727208 1105 68-69 0.2525266214896007 1105 70-71 0.25770749989429476 1105 72-73 0.2232654744841085 1105 74-75 0.21172048207358074 1105 76-77 0.26960129460627513 1105 78-79 0.10036947774210603 1105 80-81 0.09633797991310189 1105 82-83 0.22651755795188677 1105 84-85 0.25345593287504187 1105 86-87 0.4533335713930313 1105 88-89 0.2835209943812842 1105 90-91 0.3396842216849336 1105 92-93 0.19408456457792767 1105 94-95 0.14094265511352688 1105 96-97 0.12058275523947515 1105 98-99 0.19236294068000603 1105 100-101 0.22030621255798621 1105 102-103 0.24455846790978342 1105 104-105 0.32428845610622403 1105 106-107 0.2526916300775994 1105 108-109 0.31094736315293403 1105 110-111 0.33157822818579064 1105 112-113 0.4349418805100953 1105 114-115 0.39852758232208885 1105 116-117 0.44407135567325184 1105 118-119 0.44742694992392273 1105 120-121 0.4488192566465514 1105 122-123 0.45733583270253675 1105 124-125 0.29316708532580193 1106 1 0.0402462908980894 1106 2 -0.00611218988326101 1106 3 0.12673679471117794 1106 4 0.08065031245784837 1106 5 0.09533781953885523 1106 6 -0.013413810725921849 1106 7 0.0026954586499314814 1106 8 0.022517118097610478 1106 9 0.11445175938648333 1106 10-11 0.15516285044029132 1106 12-13 0.10140876988462821 1106 14-15 0.16055234374450578 1106 16-17 0.14922493045568075 1106 18-19 0.16461178542449773 1106 20-21 0.22648698976509962 1106 22-23 0.3172928720579762 1106 24-25 0.26127687139616995 1106 26-27 0.2374763300580014 1106 28-29 0.20294498179152498 1106 30-31 0.05938450304609688 1106 32-33 0.06333888024048662 1106 34-35 -0.14193005378240997 1106 36-37 -0.0382832398787869 1106 38-39 -0.037434363532312886 1106 40-41 0.0669474202495266 1106 42-43 -0.12082801330101489 1106 44-45 -0.19591606411808726 1106 46-47 -0.3136395224775228 1106 48-49 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0.14303029399623313 2205 62-63 0.21125460974586474 2205 64-65 0.24844362078619042 2205 66-67 0.11643102980920617 2205 68-69 0.2963114081315368 2205 70-71 0.40374955307920857 2205 72-73 0.439493056425988 2205 74-75 0.18632159524612035 2205 76-77 0.3293353700546362 2205 78-79 0.31973249567655415 2205 80-81 0.07119264905348643 2205 82-83 0.0658063643835547 2205 84-85 5.45723308249535E-5 2205 86-87 -0.20013581636207078 2205 88-89 -0.04338067537752721 2205 90-91 0.11951106151177271 2205 92-93 0.27343521392858605 2205 94-95 0.28898223458168104 2205 96-97 0.3550725511578463 2205 98-99 0.30088489491625126 2205 100-101 0.2445610053842131 2205 102-103 0.2051768970996477 2205 104-105 0.41891430644636074 2205 106-107 0.20992106730704307 2205 108-109 0.1899393235734621 2205 110-111 0.1965565831641527 2205 112-113 0.17453371724479894 2205 114-115 0.15724743389908014 2205 116-117 0.09195632784393837 2205 118-119 0.09396993075261406 2205 120-121 -0.1419043055055873 2205 122-123 -0.17343101949289164 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-0.18029471304063804 2206 118-119 -0.48497803639411075 2206 120-121 -0.3228134584644309 2206 122-123 -0.3822506677535351 2206 124-125 -0.15863589430326286 2207 1 0.06652901867335004 2207 2 0.032093247575907924 2207 3 0.017686088645547215 2207 4 0.1718734895019196 2207 5 0.17909831001576038 2207 6 0.10101404997059404 2207 7 0.020208901536513224 2207 8 -0.007748221869221084 2207 9 -0.08579699683242126 2207 10-11 0.09250266651145012 2207 12-13 0.041211021254042635 2207 14-15 0.0777278299498434 2207 16-17 0.09092426208038518 2207 18-19 0.2409849700066431 2207 20-21 0.10060754858714915 2207 22-23 -9.643267962360369E-4 2207 24-25 -0.07817485984660522 2207 26-27 0.02434376058214127 2207 28-29 0.01043530793856462 2207 30-31 0.11506569305564796 2207 32-33 0.16690564238933803 2207 34-35 -0.06672526962240255 2207 36-37 -0.014902667990746465 2207 38-39 -0.05440129654402881 2207 40-41 -0.27575196162896276 2207 42-43 0.013663669698132708 2207 44-45 0.05763310920720954 2207 46-47 -0.04289727412333377 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118-119 -1.2742400398027698 2216 120-121 -1.2351117377322112 2216 122-123 -1.1900570728993252 2216 124-125 -1.2948112050405491 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 2.0 5 11.0 6 29.0 7 82.0 8 116.0 9 183.0 10 368.0 11 535.0 12 719.0 13 1087.0 14 1846.0 15 1941.0 16 905.0 17 596.0 18 492.0 19 477.0 20 439.0 21 392.0 22 418.0 23 443.0 24 524.0 25 657.0 26 813.0 27 997.0 28 1223.0 29 1488.0 30 1792.0 31 2182.0 32 2877.0 33 3786.0 34 5687.0 35 12855.0 36 21310.0 37 76034.0 38 61902.0 39 804.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.56205393123137 18.431682899119274 12.274355961663575 22.731907207985785 2 15.847543601617373 19.89631722269954 40.93673700203385 23.319402173649237 3 20.488313957721523 24.871975341601342 28.92168046016067 25.718030240516466 4 12.071823338696428 16.04942472305531 37.88370024352661 33.995051694721646 5 12.411438782893299 38.212799432819736 34.43386376990351 14.941898014383458 6 31.227364966551125 36.96464808287942 17.00698105696504 14.801005893604419 7 27.277625415625078 33.78054995995437 20.566949008567338 18.374875615853213 8 26.13887338300609 35.33140790719123 19.37917141955683 19.150547290245857 9 26.524767614008688 15.495959032109313 20.321821226609714 37.657452127272286 10-11 24.96128922651263 27.076426473800453 27.00580054850375 20.956483751183168 12-13 25.98403028905662 24.376137659879134 28.165910249253695 21.473921801810548 14-15 22.577724922942505 25.130208965366602 27.545567070358956 24.746499041331944 16-17 20.978084120088344 28.650826396136203 28.15765842293037 22.213431060845085 18-19 20.627624202121204 26.13571827294129 32.252020483945344 20.984637040992162 20-21 21.473436400262116 25.260296580346093 32.864597238065194 20.401669781326603 22-23 20.750916195422665 25.551052107856226 32.74470305560275 20.953328641118365 24-25 20.53733951411305 25.395480911584105 33.10729801228066 20.959881562022183 26-27 20.467441691138994 25.77773463097347 32.785962187219376 20.968861490668157 28-29 20.357012838870954 25.923112394728538 32.962405650074025 20.757469116326483 30-31 20.780282989102737 25.62264883624979 32.766303424507925 20.83076475013955 32-33 20.65359318496226 25.479212678688445 32.97332718491372 20.893866951435573 34-35 20.873965487949906 25.83671091910783 32.64592384049705 20.64339975244521 36-37 20.923335307309213 25.647528808721738 32.512353529371794 20.91678235459726 38-39 20.602933359869326 25.749055262014615 32.73514085515474 20.91287052296132 40-41 21.079775744484625 25.527995534305752 32.38113729582797 21.011091425381647 42-43 20.696024037318033 25.627508943610355 32.30548557615296 21.37098144291865 44-45 21.132007698487243 25.906671617774478 31.9496928614248 21.01162782231348 46-47 21.17588525107395 26.14712520932942 31.4998907846516 21.17709875494503 48-49 21.277680157659187 26.04264758705719 31.408545050336382 21.271127204947238 50-51 21.37118891709666 26.56217386450238 30.83573207255923 21.230905145841728 52-53 21.297963740504333 26.57282236730335 30.856733732980608 21.272480159211707 54-55 21.32490352644225 26.5468533844623 30.68198917554547 21.446253913549985 56-57 21.190013324304843 26.611719078026063 30.84030492999279 21.357962667676304 58-59 21.317308299071406 26.3750479343336 30.89446680031649 21.4131769662785 60-61 21.500376186200036 26.362886197606972 30.98536514331481 21.151372472878187 62-63 21.211562264883625 26.552920903817682 30.97468630924933 21.260830522049364 64-65 21.280055530071913 26.16466316691301 31.12256661545668 21.4327146875584 66-67 21.12375585213662 26.548859424943387 30.816277495030693 21.511107227889298 68-69 21.303112159581 26.345718383900163 30.976460394778037 21.3747090617408 70-71 21.157734042992118 26.280189015821705 30.96990745797019 21.592169483215987 72-73 21.15404218139456 26.29323107540713 30.864985559303936 21.687741183894378 74-75 21.245297672499575 26.46093731039002 30.68029027012596 21.613474746984444 76-77 21.266149055509306 26.39671278891125 30.71809446605877 21.61904368952068 78-79 21.209429433365127 26.45954532326608 30.549063294088523 21.78196194928027 80-81 20.83950197801131 26.716258524864696 30.694366915030457 21.749872582093538 82-83 21.22199839817489 26.679610707958158 30.445113219911175 21.653277673955778 84-85 21.339708273669395 26.177462806106348 30.595830400698976 21.886998519525278 86-87 21.127881775311934 26.388919226559292 30.526734768669193 21.956464229459577 88-89 21.34189258063733 26.26968910030823 30.55991068611509 21.82850763293935 90-91 20.99483047350921 26.59636434240225 30.695095017353108 21.71371016673543 92-93 20.958284388439623 26.359130932849222 30.704223984777734 21.97836069393342 94-95 20.968861490668157 26.45025847632454 30.598014707666916 21.982865325340388 96-97 21.06230128874111 26.317501152828676 30.497051185593282 22.12314637283693 98-99 20.936096886149066 26.316287648957598 30.341965390869596 22.405650074023736 100-101 20.753343203164818 26.340315025604934 30.482489139140355 22.423852632089897 102-103 21.087784870033737 26.271873407276168 30.428124165716085 22.212217556974007 104-105 20.808678979685947 26.232313181079046 30.383709924034658 22.57529791520035 106-107 20.76620634419824 26.361672693735894 30.50797272043298 22.364148241632893 108-109 20.746062179938356 26.334975608572194 30.273523772540834 22.64543843894862 110-111 20.62471179283062 26.3806033541247 30.355071232677233 22.63961362036745 112-113 20.757954517874914 26.38472926728636 30.25459311215203 22.6027231026867 114-115 20.71359054823811 26.49158190321555 30.179429117853356 22.615398430692984 116-117 20.642771850456764 26.30403758967837 30.359344510567244 22.69384604929762 118-119 20.55874707848234 26.27582039128314 30.20151494190943 22.963917588325096 120-121 20.58253181208119 26.403238606991252 30.26097677590655 22.753252805021006 122-123 20.560638788437736 26.5327767395578 29.93349998786496 22.973084484139505 124-125 20.484965864053258 26.463185843714882 30.064145970307916 22.98770232192394 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 272.0 1 193.0 2 383.0 3 612.5 4 321.5 5 134.0 6 437.0 7 966.0 8 1633.0 9 1737.0 10 984.5 11 358.0 12 218.5 13 211.0 14 225.0 15 250.0 16 257.0 17 294.5 18 317.0 19 308.0 20 364.0 21 451.0 22 529.0 23 644.0 24 786.5 25 975.0 26 1269.0 27 1587.0 28 1837.5 29 2138.5 30 2507.0 31 2824.5 32 3204.0 33 3594.0 34 4039.0 35 4572.5 36 5150.5 37 5618.0 38 5788.5 39 6128.5 40 6520.5 41 6655.0 42 6737.0 43 6997.5 44 7406.0 45 7599.0 46 7510.0 47 7493.5 48 7680.5 49 7656.5 50 7350.0 51 7065.0 52 6875.5 53 6644.0 54 6326.0 55 5771.5 56 5083.5 57 4470.0 58 3965.0 59 3507.5 60 2947.0 61 2360.5 62 1842.5 63 1471.5 64 1112.5 65 795.5 66 565.0 67 395.5 68 302.5 69 224.0 70 156.0 71 129.0 72 113.0 73 89.5 74 74.0 75 54.5 76 35.5 77 20.5 78 15.0 79 9.0 80 2.5 81 1.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023784675873116036 2 9.70803096861879E-4 3 0.0 4 0.1397956459481106 5 0.04077373006819891 6 0.014076644904497244 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.854015484309395E-4 38-39 0.002669708516370167 40-41 0.0 42-43 9.70803096861879E-4 44-45 2.4270077421546975E-4 46-47 0.0 48-49 4.854015484309395E-4 50-51 0.001941606193723758 52-53 0.0 54-55 0.0 56-57 2.4270077421546975E-4 58-59 0.001941606193723758 60-61 0.0 62-63 0.0 64-65 2.4270077421546975E-4 66-67 2.4270077421546975E-4 68-69 2.4270077421546975E-4 70-71 2.4270077421546975E-4 72-73 0.0 74-75 0.0 76-77 0.002184306967939228 78-79 2.4270077421546975E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 2.4270077421546975E-4 88-89 0.0 90-91 0.0 92-93 4.854015484309395E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 2.4270077421546975E-4 116-117 4.854015484309395E-4 118-119 2.4270077421546975E-4 120-121 2.4270077421546975E-4 122-123 0.0 124-125 2.4270077421546975E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 206015.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.58160911275992 #Duplication Level Percentage of deduplicated Percentage of total 1 74.21449808456165 47.18677207712269 2 16.98784499152127 21.602290398381236 3 4.697625290742495 8.960477249826134 4 1.7645574397981163 4.487736055770249 5 0.803362719670146 2.5539547208915474 6 0.44734470664369613 1.706573776588904 7 0.28476155281056015 1.2673918418800505 8 0.1832345246284205 0.932027673670929 9 0.12765247960792206 0.7304715054634912 >10 0.46573435870904495 4.810401495871323 >50 0.012666252791115946 0.48382393320510464 >100 0.004871635688890748 0.701265931354902 >500 0.002922981413334449 1.1733194999877954 >1k 0.002922981413334449 3.403493839985717 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2476 1.2018542339150062 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1845 0.8955658568550833 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1173 0.5693760163094921 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 676 0.3281314467393151 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 654 0.3174526126738344 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 564 0.27376647331504983 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 421 0.20435405188942551 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 292 0.14173725214183433 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.4270077421546975E-4 0.0 0.0 0.0 0.0 14-15 4.854015484309395E-4 0.0 0.0 0.0 0.0 16-17 4.854015484309395E-4 0.0 0.0 0.0 0.0 18-19 7.281023226464093E-4 0.0 0.0 2.4270077421546975E-4 0.0 20-21 9.70803096861879E-4 0.0 0.0 4.854015484309395E-4 0.0 22-23 9.70803096861879E-4 0.0 0.0 4.854015484309395E-4 0.0 24-25 0.0014562046452928185 0.0 0.0 9.70803096861879E-4 0.0 26-27 0.0014562046452928185 0.0 0.0 9.70803096861879E-4 0.0 28-29 0.0016989054195082883 0.0 0.0 0.0012135038710773488 0.0 30-31 0.001941606193723758 0.0 0.0 0.0036405116132320462 0.0 32-33 0.002184306967939228 0.0 0.0 0.008979928645972381 0.0 34-35 0.0033978108390165765 0.0 0.0 0.015532849549790066 0.0 36-37 0.0033978108390165765 0.0 0.0 0.02815328980899449 0.0 38-39 0.004854015484309395 0.0 0.0 0.05897628813435915 0.0 40-41 0.004854015484309395 0.0 0.0 0.08785768026600005 0.0 42-43 0.005339417032740335 0.0 0.0 0.12183578865616582 0.0 44-45 0.005824818581171274 0.0 0.0 0.15290148775574594 0.0 46-47 0.005824818581171274 0.0 0.0 0.19003470621071283 0.0 48-49 0.006310220129602214 0.0 0.0 0.22741062543989515 0.0 50-51 0.006310220129602214 0.0 0.0 0.27303837099240347 0.0 52-53 0.006310220129602214 0.0 0.0 0.3176953134480499 0.0 54-55 0.006310220129602214 0.0 0.0 0.3655073659684974 0.0 56-57 0.006310220129602214 0.0 0.0 0.4254544571997184 0.0 58-59 0.006310220129602214 0.0 0.0 0.49510957939955824 0.0 60-61 0.006310220129602214 0.0 0.0 0.5773851418586026 0.0 62-63 0.006310220129602214 0.0 0.0 0.6443705555420722 0.0 64-65 0.006310220129602214 0.0 0.0 0.7154818823872049 0.0 66-67 0.006310220129602214 0.0 0.0 0.8164454044608402 0.0 68-69 0.006795621678033153 0.0 0.0 0.9251753513093707 0.0 70-71 0.007038322452248623 0.0 0.0 1.0423998252554425 0.0 72-73 0.007523724000679562 0.0 0.0 1.180496565784045 0.0 74-75 0.007766424774895032 0.0 0.0 1.3033031575370724 0.0 76-77 0.007766424774895032 0.0 0.0 1.439943693420382 0.0 78-79 0.007766424774895032 0.0 0.0 1.6057083222095478 0.0 80-81 0.008251826323325972 0.0 0.0 1.7971992330655535 0.0 82-83 0.008251826323325972 0.0 0.0 1.9952430648253767 0.0 84-85 0.008494527097541442 0.0 0.0 2.192801495036769 0.0 86-87 0.008737227871756912 0.0 0.0 2.3859913113122833 0.0 88-89 0.008737227871756912 0.0 0.0 2.6141300390748246 0.0 90-91 0.008737227871756912 0.0 0.0 2.8662961434846976 0.0 92-93 0.008979928645972381 0.0 0.0 3.153896560930029 0.0 94-95 0.009222629420187851 0.0 0.0 3.437613765987914 0.0 96-97 0.009222629420187851 0.0 0.0 3.712836443948256 0.0 98-99 0.010921534839696139 0.0 0.0 4.030774458170521 0.0 100-101 0.011164235613911609 0.0 0.0 4.405504453559207 0.0 102-103 0.011164235613911609 0.0 0.0 4.779506346625245 0.0 104-105 0.013105841807635367 0.0 0.0 5.1578768536271635 0.0 106-107 0.013833944130281776 0.0 0.0 5.5277528335315385 0.0 108-109 0.014804747227143655 0.0 0.0 5.928937213309711 0.0 110-111 0.015532849549790064 0.0 0.0 6.378661747930976 0.0 112-113 0.015532849549790064 0.0 0.0 6.8679465087493625 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 665 0.0 75.316444 1 GAATACG 30 0.004196447 59.62552 4 GGGCGAC 15 0.0042359596 59.49527 10-11 GTATCAA 1190 0.0 52.109695 1 ATCAACG 1385 0.0 44.77295 3 TCTAATA 120 4.0563464E-10 44.71914 2 TCAACGC 1390 0.0 44.611897 4 TATCAAC 1400 0.0 44.29324 2 CAACGCA 1415 0.0 43.759834 5 AACGCAG 1455 0.0 42.93473 6 ACGCAGA 1735 0.0 36.005783 7 GAGTTAG 85 1.6846131E-4 35.073833 1 CGCAGAG 1790 0.0 34.89946 8 GTACATG 955 0.0 34.339302 1 ACATGGG 925 0.0 34.163807 3 TACATGG 935 0.0 33.79842 2 GTATAAG 90 2.3568553E-4 33.125286 1 GTGGTAT 240 0.0 32.297157 1 GCAGAGT 1920 0.0 32.226604 9 GTGTAGC 190 1.0404619E-9 31.381851 1 >>END_MODULE