FastQCFastQC Report
Fri 27 May 2016
SRR940064_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940064_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815935
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13900.054.9592171
GGTATCA7800.053.5610121
TCAACGC16100.044.8020134
CAACGCA16250.044.355755
ATCAACG16300.044.2522963
AACGCAG17250.041.7844056
TATCAAC18750.039.1419372
GTACATG44500.036.7481841
GGACCGA1801.4551915E-1136.403086
TACATGG44700.035.907342
ACATGGG45500.034.3263743
ACGCAGA22300.031.7877677
CATGGGA23150.031.4157094
CGCAGAG22950.030.887468
GGTAACG1550.028.79349924-25
ACCGAGT2353.3651304E-1027.883218
CTAGTAC2153.949026E-927.726793
GAGTACA31900.027.689581
CATGGGG23950.026.8816724
GACGCAT1700.026.2384172-73
Produced by FastQC (version 0.11.5)