FastQCFastQC Report
Fri 27 May 2016
SRR940064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940064_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815935
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41960.5142566503459222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31940.3914527505254708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.23751892001201078No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.11067058037711337No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18250.075.7865141
CTATACG250.002042719271.522294
GTATCAA34450.057.1072921
TCAACGC42900.045.8476224
ATCAACG42900.045.7086873
CAACGCA43800.044.7145735
TATCAAC43800.044.6333922
TGGTATC6950.043.736652
AACGCAG45450.043.072786
GTGGTAT7350.042.9888041
GTACATG45300.038.6915361
TACATGG45750.038.0412
ACGCAGA54000.036.248487
ACATGGG46700.036.2461283
CGCAGAG54700.035.675848
GCAGAGT61150.031.5236289
CATGGGA23150.031.1526184
TAACGCA1004.393261E-429.8009554
GTGTACG1254.816206E-528.6159421
TATCACG1055.8453536E-428.3818632
Produced by FastQC (version 0.11.5)