FastQCFastQC Report
Fri 27 May 2016
SRR940063_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940063_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957031
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30500.053.6101341
GGTATCA18200.051.806221
TCAACGC37250.042.1009254
ATCAACG38300.040.9435773
CAACGCA38400.040.6577155
TATCAAC41300.039.1555332
AACGCAG40100.039.0826766
GTACATG88800.034.20591
TACATGG92100.032.395472
ACGCAGA50850.030.5859837
ACATGGG97200.029.19913
CGCAGAG52900.029.0627698
CATGGGG60600.027.7484464
GTGGTAT11150.026.2251131
GCAGAGT64950.023.0191659
GAGTACA68850.021.7553421
TATTCCG1953.9940533E-521.3912585
CCAATAC11750.020.8053383
AGTACAT66600.020.6972032
CATGGGA40600.020.2682044
Produced by FastQC (version 0.11.5)