FastQCFastQC Report
Fri 27 May 2016
SRR940063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957031
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57800.29534534710998445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40180.20531100427126603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26140.13356967774143588No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29700.066.451181
GTATCAA59500.060.7276951
TCAACGC75350.047.6235854
ATCAACG75100.047.5440033
CAACGCA76450.046.7214165
TATCAAC77300.046.3462872
AACGCAG81000.044.295916
ACGCAGA95150.037.5161557
CGCAGAG97600.036.452498
GTGGTAT12750.035.54141
GTACATG82350.033.7406541
TACATGG85800.032.2367062
GCAGAGT111300.031.5913499
ACATGGG88300.030.5806223
ATAACGC2201.546141E-1029.8042963
CCAATAC12000.027.3206023
TGGTATC16400.026.8972472
CATGGGG53250.026.7539024
TAAATCG900.009506497526.4581665
GAGTACT65250.026.03163512-13
Produced by FastQC (version 0.11.5)