FastQCFastQC Report
Fri 27 May 2016
SRR940062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927486
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14260.15374895146665288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19900.056.0737421
GGTATCA11600.049.8981861
TCAACGC24150.045.4250954
CAACGCA25250.043.4156655
ATCAACG25300.043.1200033
TATCAAC27200.040.798532
AACGCAG26700.040.165326
GTACATG57500.034.9730151
TACATGG58550.033.423162
ACGCAGA32900.033.3205347
GTACCGT902.3741713E-433.0991976
CGCAGAG33800.032.43338
ACATGGG60750.030.7146993
GGACCGT1751.6492777E-830.6404046
GTGGTAT7150.028.3755251
CATGGGG25000.027.6638834
GCAGAGT39350.027.0959539
CATGGGA37050.025.9079364
ATGGGAT15500.023.0626685
TGGTATC7400.022.5749532
Produced by FastQC (version 0.11.5)