FastQCFastQC Report
Fri 27 May 2016
SRR940062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927486
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61760.6658860618920394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46030.49628781458695875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28690.3093308146969334No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13430.14480002932658823No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9350.10081014699952345No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22500.076.3422551
GTATCAA47350.066.255321
ATCAACG63100.049.609813
TATCAAC63400.049.4691162
TCAACGC63350.049.4140364
CAACGCA64950.048.0141535
AACGCAG68050.045.901966
GTGGTAT8550.042.5518761
ACGCAGA77850.039.968677
TGGTATC8900.039.5275272
CGCAGAG79950.038.9188358
GCAGAGT87500.035.220739
GTACATG58250.033.2768671
TACATGG58600.032.255172
ACATGGG58700.031.4891723
TAGGACG850.007135430428.0594224
GAGTACT56650.027.98813412-13
AGAGTAC83600.027.82576610-11
CATGGGG25500.027.5917644
CAGAGTA85150.027.38911610-11
Produced by FastQC (version 0.11.5)