##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940062_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 927486 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.13644518623462 34.0 33.0 34.0 31.0 34.0 2 33.18531923932005 34.0 33.0 34.0 31.0 34.0 3 33.245086179198395 34.0 33.0 34.0 31.0 34.0 4 36.4692016914541 37.0 37.0 37.0 35.0 37.0 5 36.48115982343669 37.0 37.0 37.0 35.0 37.0 6 36.561154561901745 37.0 37.0 37.0 35.0 37.0 7 36.55372264379193 37.0 37.0 37.0 35.0 37.0 8 36.55313072111061 37.0 37.0 37.0 35.0 37.0 9 38.42285490023569 39.0 39.0 39.0 37.0 39.0 10-11 38.41081536540713 39.0 39.0 39.0 37.0 39.0 12-13 38.393728854128256 39.0 39.0 39.0 37.0 39.0 14-15 39.997996196169 41.0 40.0 41.0 38.0 41.0 16-17 40.00258979650367 41.0 40.0 41.0 38.0 41.0 18-19 40.054159847156726 41.0 40.0 41.0 38.0 41.0 20-21 40.05898148327846 41.0 40.0 41.0 38.0 41.0 22-23 40.01373497820991 41.0 40.0 41.0 38.0 41.0 24-25 39.9828153740326 41.0 40.0 41.0 38.0 41.0 26-27 39.88780369730648 41.0 40.0 41.0 38.0 41.0 28-29 39.806053676281905 41.0 40.0 41.0 37.0 41.0 30-31 39.643216717017836 41.0 40.0 41.0 36.5 41.0 32-33 39.45600850039785 41.0 40.0 41.0 35.0 41.0 34-35 39.3380951302769 41.0 40.0 41.0 35.0 41.0 36-37 39.22806112437277 41.0 39.5 41.0 35.0 41.0 38-39 39.10720161813764 41.0 39.0 41.0 35.0 41.0 40-41 38.97042003868522 41.0 39.0 41.0 35.0 41.0 42-43 38.79478665985255 41.0 39.0 41.0 35.0 41.0 44-45 38.556055293556994 41.0 39.0 41.0 35.0 41.0 46-47 38.21065115807678 41.0 38.5 41.0 34.5 41.0 48-49 37.88506726786173 41.0 38.0 41.0 34.0 41.0 50-51 37.87399378535094 41.0 38.0 41.0 34.0 41.0 52-53 37.765464384368066 41.0 38.0 41.0 34.0 41.0 54-55 37.600923356255514 41.0 37.5 41.0 34.0 41.0 56-57 37.40556461229603 40.5 37.0 41.0 33.5 41.0 58-59 37.18885837629894 40.0 36.5 41.0 33.0 41.0 60-61 36.946618601251124 40.0 36.0 41.0 33.0 41.0 62-63 36.68087119374309 39.0 35.5 41.0 33.0 41.0 64-65 36.37902674541718 39.0 35.0 41.0 32.5 41.0 66-67 36.052498366552165 38.5 35.0 41.0 32.0 41.0 68-69 35.70012054090304 37.0 35.0 40.5 32.0 41.0 70-71 35.336684866402294 37.0 35.0 39.5 31.0 41.0 72-73 34.97724655682134 36.0 35.0 39.0 31.0 41.0 74-75 34.62000935863183 36.0 35.0 39.0 31.0 41.0 76-77 34.26836200222968 35.5 35.0 37.5 31.0 39.5 78-79 33.943644432368785 35.0 35.0 37.0 31.0 39.0 80-81 33.638044671294224 35.0 35.0 37.0 30.0 39.0 82-83 33.36078388245214 35.0 35.0 36.0 30.0 38.0 84-85 33.12545041111133 35.0 35.0 36.0 30.0 37.0 86-87 32.92076376355007 35.0 35.0 35.5 29.5 37.0 88-89 32.81722311711444 35.0 35.0 35.0 29.5 36.0 90-91 32.81285701347514 35.0 35.0 35.0 30.0 36.0 92-93 32.730930709466236 35.0 35.0 35.0 30.0 36.0 94-95 32.65271173904512 35.0 35.0 35.0 30.0 36.0 96-97 32.59053236383083 35.0 35.0 35.0 30.0 36.0 98-99 32.53708789135362 35.0 35.0 35.0 30.0 35.5 100-101 32.47883903368892 35.0 35.0 35.0 30.0 35.0 102-103 32.01421207436015 35.0 33.5 35.0 28.0 35.0 104-105 32.38734115663202 35.0 34.5 35.0 29.0 35.0 106-107 32.42821832351108 35.0 35.0 35.0 30.0 35.0 108-109 32.41319815069985 35.0 35.0 35.0 30.0 35.0 110-111 32.37242934125151 35.0 35.0 35.0 29.5 35.0 112-113 32.34020028334659 35.0 35.0 35.0 29.5 35.0 114-115 32.286271167435416 35.0 35.0 35.0 29.0 35.0 116-117 32.2352008547838 35.0 35.0 35.0 29.0 35.0 118-119 32.17689916613297 35.0 35.0 35.0 29.0 35.0 120-121 32.09975083181848 35.0 35.0 35.0 29.0 35.0 122-123 32.000210245761124 35.0 35.0 35.0 29.0 35.0 124-125 30.983918355640945 34.5 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11807185591264613 1101 2 0.022237159499077563 1101 3 0.07233242634570303 1101 4 0.03925080546161297 1101 5 0.007071882282801312 1101 6 0.07233647810638644 1101 7 0.09282523857896052 1101 8 0.08387697679577144 1101 9 0.15217638216034857 1101 10-11 0.1159990564489135 1101 12-13 0.1375182226183682 1101 14-15 0.2639094844481207 1101 16-17 0.24520302097338487 1101 18-19 0.20248610968826597 1101 20-21 0.17880557841584732 1101 22-23 0.20016137937479073 1101 24-25 0.2204182121205136 1101 26-27 0.2981343016038309 1101 28-29 0.24288745984911486 1101 30-31 0.3318904230317301 1101 32-33 0.2925098733956659 1101 34-35 0.3490744923644442 1101 36-37 0.3042225858997796 1101 38-39 0.3006797985195746 1101 40-41 0.27565150830811547 1101 42-43 0.15328560916059075 1101 44-45 0.23044775262787454 1101 46-47 0.3864006084006988 1101 48-49 0.5177650249562049 1101 50-51 0.4456611204091274 1101 52-53 0.48422240729018995 1101 54-55 0.5058415867138919 1101 56-57 0.43514666201671304 1101 58-59 0.45776138607944716 1101 60-61 0.49365071115768444 1101 62-63 0.475529641101744 1101 64-65 0.5516727912940311 1101 66-67 0.4320401061164105 1101 68-69 0.40822629008442846 1101 70-71 0.4821186820269716 1101 72-73 0.4760899219845953 1101 74-75 0.4291634971279379 1101 76-77 0.39625978663387684 1101 78-79 0.39119649436214843 1101 80-81 0.44304728198817855 1101 82-83 0.387059273483942 1101 84-85 0.3508666379152814 1101 86-87 0.42993276409951164 1101 88-89 0.3910009196539619 1101 90-91 0.40435690022768256 1101 92-93 0.4260013064262296 1101 94-95 0.4379677743361725 1101 96-97 0.3805785464741689 1101 98-99 0.4117115444417436 1101 100-101 0.3887431346317385 1101 102-103 0.33951355586668797 1101 104-105 0.3517998391221866 1101 106-107 0.35107682304592913 1101 108-109 0.4203658573730209 1101 110-111 0.40560398225044736 1101 112-113 0.35749886975515466 1101 114-115 0.40511624121678125 1101 116-117 0.3798986141396128 1101 118-119 0.323406295915035 1101 120-121 0.3214036364409125 1101 122-123 0.25125966983634385 1101 124-125 0.04618602014233275 1102 1 0.12223264674215528 1102 2 0.13515562382285395 1102 3 0.09997329517840114 1102 4 0.025467604072815675 1102 5 0.044144342408550585 1102 6 0.06742869966151233 1102 7 0.1476345822072318 1102 8 0.14585833235722134 1102 9 0.15741693078900454 1102 10-11 0.18438914687862962 1102 12-13 0.19631382725946622 1102 14-15 0.24602483236749606 1102 16-17 0.26909847994026137 1102 18-19 0.2724143895689366 1102 20-21 0.26743996404762527 1102 22-23 0.23485092573689315 1102 24-25 0.23099006488251206 1102 26-27 0.2148521636833891 1102 28-29 0.3606508718837631 1102 30-31 0.17514868527324978 1102 32-33 0.24114342128431332 1102 34-35 0.14520621350768437 1102 36-37 0.16140832751187872 1102 38-39 0.21997953835379036 1102 40-41 0.1640652882516207 1102 42-43 0.14299300410527138 1102 44-45 0.2436073008050883 1102 46-47 0.21727888897948588 1102 48-49 0.17773440079103864 1102 50-51 0.0643821193712526 1102 52-53 0.05927837318473905 1102 54-55 -0.02782405139038957 1102 56-57 -0.07696338019623283 1102 58-59 0.07705833343659663 1102 60-61 0.1519853243763407 1102 62-63 0.17081803417082142 1102 64-65 0.09435929385612951 1102 66-67 0.09914193379264447 1102 68-69 0.16837810775429318 1102 70-71 0.10888201494143601 1102 72-73 0.1985246827391265 1102 74-75 0.09622576471239341 1102 76-77 0.11052771550016871 1102 78-79 0.12215296429880595 1102 80-81 0.05208769378546663 1102 82-83 -0.08701606754731728 1102 84-85 -0.05436250809868426 1102 86-87 -0.001011605103194313 1102 88-89 -0.12843239366661408 1102 90-91 0.0014792526103661885 1102 92-93 0.05948636434685994 1102 94-95 0.018513284731170643 1102 96-97 0.09839524827950186 1102 98-99 0.1575193382452369 1102 100-101 0.17639736081306978 1102 102-103 0.1494971267932783 1102 104-105 0.11954254028525213 1102 106-107 0.13671464807863032 1102 108-109 0.06918161439028125 1102 110-111 0.0726371615321284 1102 112-113 0.024529721972612606 1102 114-115 0.058667573926456384 1102 116-117 0.1637073666935649 1102 118-119 0.1283610810571929 1102 120-121 0.13738172480419308 1102 122-123 0.07062480017521011 1102 124-125 0.08000524448528878 1103 1 0.16019522820679555 1103 2 0.12442266396161727 1103 3 0.1599033507287686 1103 4 -0.012023774275967014 1103 5 0.09476946428777921 1103 6 0.12610069600722085 1103 7 0.12099529236571271 1103 8 0.13490911985827836 1103 9 0.19769075974303263 1103 10-11 0.17631761202363805 1103 12-13 0.24794812811468603 1103 14-15 0.2273330166572265 1103 16-17 0.23954888486384363 1103 18-19 0.29003139173070736 1103 20-21 0.223593034493625 1103 22-23 0.24231505625331806 1103 24-25 0.20482766901573513 1103 26-27 0.22684432092106022 1103 28-29 0.35184331927936086 1103 30-31 0.32832536064314155 1103 32-33 0.3858289558039303 1103 34-35 0.31831417365345516 1103 36-37 0.39843487650675513 1103 38-39 0.47389769469761944 1103 40-41 0.3997654877351948 1103 42-43 0.35696816009171783 1103 44-45 0.32702846306057864 1103 46-47 0.4495437066481571 1103 48-49 0.41198570760192865 1103 50-51 0.536001806100856 1103 52-53 0.4548537683390137 1103 54-55 0.5599748989117188 1103 56-57 0.6076139369507843 1103 58-59 0.5221904865616835 1103 60-61 0.4621405482433474 1103 62-63 0.44437458575313826 1103 64-65 0.5102336032704002 1103 66-67 0.46124673209723 1103 68-69 0.4776944990213252 1103 70-71 0.502470541562893 1103 72-73 0.47699206615050116 1103 74-75 0.3791251890251104 1103 76-77 0.3671289221140128 1103 78-79 0.41571212608840824 1103 80-81 0.39860560898863184 1103 82-83 0.4606065571830271 1103 84-85 0.4497330133471209 1103 86-87 0.4661988041606193 1103 88-89 0.45694680343665794 1103 90-91 0.41839802432573947 1103 92-93 0.3843744630916035 1103 94-95 0.32489243403123425 1103 96-97 0.32285258694554386 1103 98-99 0.3248772545714047 1103 100-101 0.35720732937677013 1103 102-103 0.3761075070716373 1103 104-105 0.38029314180903384 1103 106-107 0.37096595488952033 1103 108-109 0.3233569975558126 1103 110-111 0.4318085117756567 1103 112-113 0.4171379033031073 1103 114-115 0.4634572284002658 1103 116-117 0.4439112208467151 1103 118-119 0.4306208073041162 1103 120-121 0.3928773872315503 1103 122-123 0.4544390869626298 1103 124-125 0.486043852789539 1104 1 0.14126142060853653 1104 2 0.20936961656006758 1104 3 0.1999250096831986 1104 4 -0.003388340256151423 1104 5 0.0696853728786948 1104 6 0.15990540542242826 1104 7 0.1788781814213536 1104 8 0.200083017410833 1104 9 0.25026557535674243 1104 10-11 0.24438295036332391 1104 12-13 0.2514169681828591 1104 14-15 0.2741923571553997 1104 16-17 0.2240750285699633 1104 18-19 0.23682065914101713 1104 20-21 0.19572713038412815 1104 22-23 0.1398135359043522 1104 24-25 0.12828467221347495 1104 26-27 0.21139416799728394 1104 28-29 0.3074917212648103 1104 30-31 0.2607807557153592 1104 32-33 0.2293674541509958 1104 34-35 0.17386583625968655 1104 36-37 0.03594331157322017 1104 38-39 0.15760251662975833 1104 40-41 0.21897284703902642 1104 42-43 0.10177545539644939 1104 44-45 0.09018587047484772 1104 46-47 0.0913583774046387 1104 48-49 -0.06704680971819243 1104 50-51 -0.005090208159685972 1104 52-53 -0.002870900721198666 1104 54-55 0.016461807614483348 1104 56-57 -0.03698254650612398 1104 58-59 -0.04650336145083145 1104 60-61 -0.08291066955433024 1104 62-63 -0.062207543831704015 1104 64-65 3.8337153292644643E-4 1104 66-67 -0.017407642396754852 1104 68-69 0.015444708462823087 1104 70-71 0.06491892191902338 1104 72-73 -0.05137271982658831 1104 74-75 -4.598390374539463E-4 1104 76-77 0.015793496464446832 1104 78-79 -0.016311793574942612 1104 80-81 -0.01493412637805136 1104 82-83 0.026217485621813807 1104 84-85 0.05628669888784543 1104 86-87 0.012612225008773237 1104 88-89 -0.07067465966867559 1104 90-91 -0.06723921485385631 1104 92-93 -0.04463690637800255 1104 94-95 -0.04634345227851355 1104 96-97 0.0012534094524880857 1104 98-99 -0.07224567480959365 1104 100-101 0.01883875826776915 1104 102-103 0.08882636510252695 1104 104-105 0.06208021295395838 1104 106-107 0.018101661868463736 1104 108-109 -0.036212015917335805 1104 110-111 -0.041274089284030424 1104 112-113 -0.03876864319734352 1104 114-115 -0.05272772899662925 1104 116-117 0.0303059308470921 1104 118-119 0.0024590555065486797 1104 120-121 0.0373397985013213 1104 122-123 0.033845006434873426 1104 124-125 0.03653203110406622 1105 1 0.07766035097261437 1105 2 0.10709101881347038 1105 3 0.09859564977120527 1105 4 -0.033528420436148565 1105 5 -0.010301078779193062 1105 6 0.04969206172859231 1105 7 0.027444860754009426 1105 8 0.0290842968183469 1105 9 0.025200146257120082 1105 10-11 0.11073431921550991 1105 12-13 0.146856357141246 1105 14-15 0.23923172846679108 1105 16-17 0.17894033499447204 1105 18-19 0.18204522433458692 1105 20-21 0.21558036142178594 1105 22-23 0.22217877472945702 1105 24-25 0.17356630552873042 1105 26-27 0.2908675758180408 1105 28-29 0.3317990409274145 1105 30-31 0.34340073752756695 1105 32-33 0.29037128383466637 1105 34-35 0.33219643003855026 1105 36-37 0.3749236849780573 1105 38-39 0.3659235753359198 1105 40-41 0.31328396649925594 1105 42-43 0.3222946472207653 1105 44-45 0.15743339384644628 1105 46-47 0.20184816704955466 1105 48-49 0.3400538308394516 1105 50-51 0.37566765934265334 1105 52-53 0.3228361533825108 1105 54-55 0.3104327242196021 1105 56-57 0.23042429796830532 1105 58-59 0.34318225154760285 1105 60-61 0.4400247381554152 1105 62-63 0.2986985157781561 1105 64-65 0.2277962786058083 1105 66-67 0.2607670742906194 1105 68-69 0.13765552180452545 1105 70-71 0.14423720691522846 1105 72-73 0.2734434453948751 1105 74-75 0.25792853443568475 1105 76-77 0.2801377325129977 1105 78-79 0.22367887059267844 1105 80-81 0.22104824939204093 1105 82-83 0.28273193268010743 1105 84-85 0.20615242514018206 1105 86-87 0.1488773085687356 1105 88-89 0.2361625567162804 1105 90-91 0.19378980449948813 1105 92-93 0.16162237074963315 1105 94-95 0.23930064188246547 1105 96-97 0.17194401911365276 1105 98-99 0.282025681974325 1105 100-101 0.30474471114173696 1105 102-103 0.33166011755211855 1105 104-105 0.25453320291082093 1105 106-107 0.20565049353742637 1105 108-109 0.21124090999798995 1105 110-111 0.270447361356311 1105 112-113 0.2506724023020297 1105 114-115 0.27947242498768077 1105 116-117 0.34451895291011425 1105 118-119 0.33768446035577426 1105 120-121 0.35894428083937413 1105 122-123 0.3254093845417785 1105 124-125 0.33034746909834567 1106 1 0.13095751673509426 1106 2 0.07906531817095441 1106 3 0.11400020155166857 1106 4 -0.08682558619862846 1106 5 -0.011751115030101289 1106 6 0.0508795914168374 1106 7 0.0637416015058605 1106 8 0.11179886468254097 1106 9 0.0437922777270785 1106 10-11 0.10860509931835338 1106 12-13 0.13669568645781993 1106 14-15 0.1113035302618357 1106 16-17 0.1909156343769567 1106 18-19 0.18896414730765798 1106 20-21 0.16945837503879346 1106 22-23 0.1946364195039081 1106 24-25 0.2189549402446005 1106 26-27 0.1868524754405314 1106 28-29 0.20738759730798506 1106 30-31 0.16621922465640893 1106 32-33 0.18314360314265343 1106 34-35 0.16173800191118204 1106 36-37 0.2615083495946777 1106 38-39 0.26458354183508703 1106 40-41 0.17905561079035692 1106 42-43 0.24789278717426555 1106 44-45 0.1275243127860861 1106 46-47 0.06564476196442826 1106 48-49 0.05475919847363997 1106 50-51 0.008816821405034148 1106 52-53 0.05209605154662711 1106 54-55 0.04319270988590773 1106 56-57 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-0.924012854143534 2216 124-125 -1.0342956351572887 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 16.0 5 43.0 6 111.0 7 280.0 8 452.0 9 746.0 10 1394.0 11 2188.0 12 2805.0 13 4573.0 14 8509.0 15 9342.0 16 4013.0 17 3092.0 18 2845.0 19 2322.0 20 2113.0 21 1869.0 22 1844.0 23 2024.0 24 2298.0 25 2851.0 26 3581.0 27 4266.0 28 5221.0 29 6197.0 30 7843.0 31 9636.0 32 12605.0 33 16448.0 34 24449.0 35 45407.0 36 113396.0 37 365786.0 38 254678.0 39 2237.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.11606088545964 18.704000258811732 14.003979230358615 22.17595962537002 2 15.752309523604149 19.703621919556983 41.00353221257105 23.54053634426781 3 19.38081950739851 25.410249664684244 29.0622803196605 26.146650508256748 4 12.578832975046616 16.450273006919918 37.095037935923 33.87585608211046 5 13.22080225047784 38.110996535403174 33.43702674599716 15.231174468121825 6 31.340632844782224 36.52767833265759 17.166432311660525 14.965256510899664 7 28.38242302309684 33.480613184457766 20.071138540096563 18.065825252348823 8 26.806118906377023 35.22522172841423 18.89311536777914 19.075543997429612 9 27.312865099850566 16.167683393603784 19.855070588666567 36.664380917879086 10-11 25.33402121433639 27.125638554112946 26.17818489982598 21.362155331724683 12-13 26.316030646284688 24.384411193268683 27.43723355392966 21.86232460651697 14-15 23.228221234606234 25.12981328020046 26.350155150589877 25.291810334603433 16-17 21.802754974199072 28.807281188071844 26.81496001017805 22.575003827551036 18-19 21.643237741593943 26.388646297626057 30.434852925003717 21.533263035776283 20-21 22.717054489232183 25.366744080234092 30.78434607099191 21.131855359541817 22-23 22.394246382155632 25.383725468632413 30.65836034182726 21.563667807384693 24-25 21.844642398914914 25.58631612768279 30.792809810606304 21.77623166279599 26-27 21.70329255643751 25.910310236488744 30.623535018318336 21.76286218875541 28-29 21.595670871049595 25.871968749882075 30.895341108126335 21.63701927094199 30-31 21.867169676242394 25.57111497341194 30.773738414894492 21.787976935451177 32-33 21.790217631551993 25.660684539372603 30.611384550693543 21.93771327838186 34-35 22.06057796238743 25.785607814702704 30.409953826622065 21.7438603962878 36-37 22.247482003528944 25.537710780954924 30.334828973550188 21.87997824196594 38-39 21.958149025696592 25.70640703212816 30.36575799969271 21.96968594248254 40-41 22.116626082025 25.622934266896895 30.295514053858525 21.96492559721958 42-43 22.077600720663607 25.586630849124802 30.061020282410862 22.27474814780073 44-45 22.13880622762755 25.79117609005046 29.93595549230172 22.13406219002027 46-47 22.50773731772626 26.19841496174334 29.160455877746877 22.133391842783524 48-49 22.58636137703794 26.240391862153885 28.815302721708218 22.35794403909996 50-51 22.65240638251762 26.66708716498953 28.43851721509352 22.241989237399338 52-53 22.78634005599009 26.466020223496756 28.439366437534602 22.308273282978547 54-55 22.441147359636695 26.557813271574986 28.428677090543687 22.572362278244633 56-57 22.49676136299312 26.684050811155252 28.46647370517864 22.35271412067299 58-59 22.580629509928922 26.27526031909474 28.510540800621047 22.633569370355293 60-61 22.59299052749216 26.47013634351817 28.739203869436704 22.197669259552967 62-63 22.46471231024595 26.460794181244196 28.828383469894614 22.246110038615242 64-65 22.34436381338375 26.238590668971852 28.750031671995647 22.667013845648754 66-67 22.360775823495736 26.566423606356587 28.523718596324017 22.549081973823665 68-69 22.406054274122162 26.3908970249397 28.68771947983616 22.51532922110198 70-71 22.310053138812684 26.404604321511112 28.623043279263676 22.662299260412528 72-73 22.37058025673703 26.560616548389948 28.33708541153182 22.731717783341203 74-75 22.400242375756818 26.945272998877073 28.062541139457164 22.591943485908946 76-77 22.534945506143767 26.657782033200927 28.108597392403205 22.6986750682521 78-79 22.44259294699871 26.750298524762055 28.144294605529513 22.662813922709717 80-81 22.502657722057258 26.90218504645892 27.94273983650427 22.652417394979548 82-83 22.507455638144403 26.806280633885578 27.947537752591412 22.738725975378603 84-85 22.623445725391043 26.69689185510239 27.88586307558364 22.79379934392293 86-87 22.520490963706035 26.694210777136373 28.02194543937241 22.763352819785183 88-89 22.47710477570551 26.758840564709335 27.932497094295762 22.831557565289394 90-91 22.498004009770515 26.841041548403233 27.960844628670344 22.70010981315591 92-93 22.408377979447515 26.718718159656447 27.945322751242884 22.927581109653154 94-95 22.435782704889398 26.58354501895467 28.02859588819549 22.952076387960442 96-97 22.534921219367952 26.531878567797385 28.038756609977245 22.894443602857418 98-99 22.382655910709165 26.51215220499285 27.759394750971982 23.345797133326002 100-101 22.21883192531188 26.489366118103447 28.03710254683776 23.254699409746912 102-103 22.36734570656592 26.242498539061504 28.02904841690333 23.361107337469246 104-105 22.2820074912182 26.36039789279838 27.97675652247042 23.380838093513002 106-107 22.29779705094643 26.376142337765394 27.8610736142079 23.464986997080274 108-109 22.405490130541548 26.371117514346633 27.587582515033976 23.635809840077844 110-111 22.191224449749107 26.645577399551044 27.738477993198817 23.42472015750103 112-113 22.247403862171133 26.51615904757335 27.82712576557966 23.40931132467586 114-115 22.034770007741525 26.677312160230134 27.6914194159139 23.596498416114446 116-117 21.993117884927543 26.608005701492164 27.814451363457728 23.584425050122565 118-119 21.927637448685015 26.779049739482957 27.577501462291742 23.71581134954029 120-121 21.869368167338646 26.75893775044219 27.748496066511088 23.62319801570808 122-123 21.847474211103712 26.705948629758513 27.60229977385018 23.844277385287594 124-125 21.821410511720483 26.837696594747694 27.55345404046884 23.78743885306298 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 151.0 1 129.5 2 270.5 3 428.0 4 260.5 5 169.0 6 502.0 7 1389.5 8 2565.0 9 2862.0 10 1983.0 11 1152.0 12 1005.0 13 1104.0 14 1210.0 15 1322.0 16 1396.0 17 1479.5 18 1596.5 19 1738.0 20 1906.5 21 2095.5 22 2416.5 23 2957.5 24 3573.0 25 4221.0 26 5304.5 27 6540.5 28 7593.5 29 8936.0 30 10657.0 31 12485.5 32 14561.5 33 16370.5 34 17987.5 35 19298.5 36 21257.5 37 23144.5 38 23993.5 39 24953.5 40 25591.0 41 26646.0 42 27697.0 43 28650.5 44 30252.5 45 32021.0 46 32575.0 47 32772.5 48 33313.5 49 33292.0 50 32921.5 51 32658.5 52 32748.0 53 33329.5 54 33635.5 55 31545.0 56 27907.5 57 24879.0 58 22980.5 59 20563.5 60 17501.0 61 14443.5 62 11669.5 63 9212.0 64 6914.0 65 5152.0 66 3697.0 67 2855.0 68 2113.0 69 1402.5 70 948.0 71 687.5 72 548.5 73 461.5 74 348.0 75 250.0 76 189.5 77 104.0 78 53.5 79 25.0 80 13.5 81 8.0 82 4.0 83 1.5 84 1.5 85 3.0 86 2.5 87 1.0 88 1.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01843693597531391 2 0.0023720034588123164 3 2.1563667807384697E-4 4 0.14221238918970205 5 0.04248042558054785 6 0.012722564006356967 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.6172750855538521E-4 30-31 2.1563667807384697E-4 32-33 1.0781833903692348E-4 34-35 3.7736418662923213E-4 36-37 0.0013477292379615435 38-39 0.0030728226625523184 40-41 5.390916951846174E-5 42-43 8.086375427769259E-4 44-45 6.469100342215409E-4 46-47 5.390916951846174E-5 48-49 0.001832911763627699 50-51 0.0016172750855538518 52-53 5.390916951846174E-5 54-55 0.0 56-57 0.0013477292379615435 58-59 0.001994639272183084 60-61 0.0013477292379615435 62-63 2.6954584759230867E-4 64-65 0.0011320925598876964 66-67 7.547283732584643E-4 68-69 9.703650513323113E-4 70-71 0.001024274220850773 72-73 0.0 74-75 5.390916951846174E-5 76-77 0.004528370239550786 78-79 9.164558818138495E-4 80-81 0.0 82-83 0.0 84-85 3.7736418662923213E-4 86-87 4.3127335614769394E-4 88-89 0.0 90-91 1.6172750855538521E-4 92-93 7.547283732584643E-4 94-95 0.0010781833903692347 96-97 5.930008647030791E-4 98-99 0.0 100-101 2.6954584759230867E-4 102-103 0.0 104-105 0.0 106-107 2.1563667807384697E-4 108-109 3.7736418662923213E-4 110-111 0.0 112-113 2.6954584759230867E-4 114-115 0.0021563667807384694 116-117 0.001024274220850773 118-119 3.7736418662923213E-4 120-121 1.6172750855538521E-4 122-123 3.7736418662923213E-4 124-125 7.547283732584643E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 927486.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.64343087152612 #Duplication Level Percentage of deduplicated Percentage of total 1 76.46850527573578 44.07906997710806 2 13.335997043523559 15.374652473624542 3 4.355515824512287 7.5320062602033655 4 1.9343101359824106 4.460010904303776 5 1.060964786137291 3.057882515341422 6 0.6322960462379587 2.1868628058994237 7 0.42867377667291184 1.7297159048456718 8 0.31733216886376103 1.463369195136773 9 0.21099382538155415 1.0946167188930425 >10 1.1663154887120866 11.998603231511096 >50 0.06120983935708265 2.3618715253824494 >100 0.02676287125815098 2.886366238592983 >500 3.743058750500176E-4 0.15772256903107054 >1k 5.614588125750264E-4 0.9509743132755724 >5k 1.871529375250088E-4 0.6662753668508151 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6176 0.6658860618920394 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4603 0.49628781458695875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2869 0.3093308146969334 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1343 0.14480002932658823 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 935 0.10081014699952345 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.3909169518461736E-5 0.0 14-15 0.0 0.0 0.0 1.0781833903692347E-4 0.0 16-17 0.0 0.0 0.0 1.0781833903692347E-4 0.0 18-19 0.0 0.0 0.0 2.1563667807384694E-4 0.0 20-21 0.0 0.0 0.0 2.1563667807384694E-4 0.0 22-23 0.0 0.0 0.0 5.390916951846173E-4 0.0 24-25 0.0 0.0 0.0 6.469100342215409E-4 0.0 26-27 0.0 0.0 0.0 8.08637542776926E-4 0.0 28-29 0.0 0.0 0.0 0.0015094567465169285 0.0 30-31 0.0 0.0 0.0 0.00495964359569848 0.0 32-33 0.0 0.0 0.0 0.009865378021878497 0.0 34-35 0.0 0.0 0.0 0.017197025076389295 0.0 36-37 0.0 0.0 0.0 0.029272679048524722 0.0 38-39 0.0 0.0 0.0 0.05002770931313249 0.0 40-41 0.0 0.0 0.0 0.07234610549377565 0.0 42-43 0.0 0.0 0.0 0.0943949558268265 0.0 44-45 0.0 0.0 0.0 0.12140344975557582 0.0 46-47 0.0 0.0 0.0 0.153533314788579 0.0 48-49 0.0 0.0 0.0 0.18916727584028223 0.0 50-51 0.0 0.0 0.0 0.23272588481119932 0.0 52-53 0.0 0.0 0.0 0.27326558028908254 0.0 54-55 0.0 0.0 0.0 0.31585382420866726 0.0 56-57 0.0 0.0 0.0 0.3674988086073536 0.0 58-59 0.0 0.0 0.0 0.43035690026588 0.0 60-61 0.0 0.0 0.0 0.5009779123350648 0.0 62-63 0.0 0.0 0.0 0.5681487375550682 0.0 64-65 0.0 0.0 0.0 0.6348343802494054 0.0 66-67 0.0 0.0 0.0 0.7172615004431334 0.0 68-69 0.0 0.0 0.0 0.8173169190693983 0.0 70-71 0.0 0.0 0.0 0.9329520876864987 0.0 72-73 0.0 0.0 0.0 1.0465926170314162 0.0 74-75 0.0 0.0 0.0 1.1533327726779703 0.0 76-77 0.0 0.0 0.0 1.2773777717399506 0.0 78-79 0.0 0.0 0.0 1.431989269918899 0.0 80-81 0.0 0.0 0.0 1.6019109722410905 0.0 82-83 0.0 0.0 0.0 1.7839083285354174 0.0 84-85 0.0 0.0 0.0 1.954369122552793 0.0 86-87 0.0 0.0 0.0 2.1358273871519353 0.0 88-89 0.0 0.0 0.0 2.3563158904824437 0.0 90-91 0.0 0.0 0.0 2.6092577138630664 0.0 92-93 0.0 0.0 0.0 2.8752455562671564 0.0 94-95 0.0 0.0 0.0 3.1403708519589513 0.0 96-97 0.0 0.0 0.0 3.4064126035325604 0.0 98-99 0.0 0.0 0.0 3.694772751286812 0.0 100-101 0.0 0.0 0.0 4.028632238114645 0.0 102-103 0.0 0.0 0.0 4.396238865061036 0.0 104-105 0.0 0.0 0.0 4.764492402041648 0.0 106-107 0.0 0.0 0.0 5.122503196813753 0.0 108-109 0.0 0.0 0.0 5.498843109222134 0.0 110-111 0.0 0.0 0.0 5.935561291491192 0.0 112-113 0.0 0.0 0.0 6.407266524777732 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2250 0.0 76.342255 1 GTATCAA 4735 0.0 66.25532 1 ATCAACG 6310 0.0 49.60981 3 TATCAAC 6340 0.0 49.469116 2 TCAACGC 6335 0.0 49.414036 4 CAACGCA 6495 0.0 48.014153 5 AACGCAG 6805 0.0 45.90196 6 GTGGTAT 855 0.0 42.551876 1 ACGCAGA 7785 0.0 39.96867 7 TGGTATC 890 0.0 39.527527 2 CGCAGAG 7995 0.0 38.918835 8 GCAGAGT 8750 0.0 35.22073 9 GTACATG 5825 0.0 33.276867 1 TACATGG 5860 0.0 32.25517 2 ACATGGG 5870 0.0 31.489172 3 TAGGACG 85 0.0071354304 28.059422 4 GAGTACT 5665 0.0 27.988134 12-13 AGAGTAC 8360 0.0 27.825766 10-11 CATGGGG 2550 0.0 27.591764 4 CAGAGTA 8515 0.0 27.389116 10-11 >>END_MODULE