FastQCFastQC Report
Fri 27 May 2016
SRR940061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855543
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19730.2306137739423968No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.061.884391
GTATCAA21500.051.3475951
TCAACGC24250.043.7584884
ATCAACG24900.042.6161963
CAACGCA24800.042.5252275
AACGCAG25350.040.1923336
TATCAAC27700.038.981482
TATTCCG1051.4690901E-534.0476385
GTACATG52450.033.449431
GTATAGG2350.033.0112761
TACATGG53350.032.3163952
ACGCAGA31300.031.7904877
CGCAGAG32250.030.6692688
ACATGGG57050.029.1542973
CATGGGA33500.028.1168294
GCAGAGT36050.026.9311459
GTAAGGT3059.094947E-1225.4095824
GGTAAGG3650.024.499233
TAAGGTG3950.024.1350355
ATGGGAT13350.024.1011375
Produced by FastQC (version 0.11.5)