FastQCFastQC Report
Fri 27 May 2016
SRR940061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855543
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68600.8018299489330168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52540.6141129084102143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33910.39635646601047525No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14530.16983366119528767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10430.12191087999083622No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27250.070.4289551
GTATCAA51150.057.4464451
ATCAACG63550.045.862233
TCAACGC64250.045.3625644
CAACGCA64950.044.8185585
TATCAAC65650.044.758352
AACGCAG66650.043.654986
GTGGTAT11550.043.3470151
TATAACG853.4390378E-642.07212
TGGTATC12150.041.206422
ACGCAGA77750.037.418197
CGCAGAG78750.036.7919438
GCAGAGT84600.034.0368469
GTACATG51050.032.2236861
TAACGCA1152.7309568E-531.0967714
TACATGG53300.030.7515742
GAGTACT56350.030.670912-13
GGACCGA2651.8189894E-1229.1892246
ACATGGG54400.028.4863193
GTACTTT59850.028.42995614-15
Produced by FastQC (version 0.11.5)