FastQCFastQC Report
Fri 27 May 2016
SRR940060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241106
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22750.051.9456861
ATCAACG25350.045.154143
TCAACGC25550.044.567344
CAACGCA25900.043.9331445
GGTATCA14000.043.911161
AACGCAG26950.042.000416
TATCAAC29050.040.264562
GTACATG58700.034.062181
GTTCGCG700.002772067434.0424357
TTCGCGC700.002772067434.0424358
ACGCAGA33200.033.734827
CGCAGAG33700.033.2343068
TACATGG59250.033.1375662
ACATGGG59800.031.4039063
CGTAAGA1954.6482455E-827.5436132
GCAGAGT41150.026.4839179
CATGGGA31500.026.3074364
TAGCCCT9550.025.594984
CCGTAAG2151.1957127E-724.9844471
CATGGGG31400.024.3027064
Produced by FastQC (version 0.11.5)