FastQCFastQC Report
Fri 27 May 2016
SRR940060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241106
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34520.27813901471751806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26760.2156141377126531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15110.12174624891024617No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23450.067.885011
GTATCAA42600.058.642151
TCAACGC53500.046.6831864
ATCAACG54250.045.8180473
CAACGCA55200.045.0774545
TATCAAC55750.044.5852742
AACGCAG57350.043.3683056
ACGCAGA67050.037.0883187
CGCAGAG68600.036.1635938
GTGGTAT10950.034.8474731
GCAGAGT78100.031.7646929
GTACATG58800.031.2304941
TACATGG60400.029.8037072
TATAACG1602.5624286E-729.8037072
ACATGGG60300.028.8646163
TGGTATC12800.027.009612
ATAACGC1556.73682E-626.9194773
TAACGCA1358.146135E-526.4921844
CAGAGTA76650.025.76828610-11
GAGTACT46700.025.7332212-13
Produced by FastQC (version 0.11.5)