FastQCFastQC Report
Fri 27 May 2016
SRR940059_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940059_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823543
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10020.12166942102598165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10500.058.542321
TAGCGAT350.0077610951.057056
GTATCAA18100.046.820041
ATCAACG19600.043.4883653
TCAACGC19950.042.7306184
CAACGCA20550.041.450215
AACGCAG22100.039.082136
GTACATG48800.036.198781
TATCAAC24100.035.4069142
TACATGG51050.034.014682
ACATGGG51000.031.9069733
ACGCAGA27950.030.6890327
CGCAGAG27850.030.3714628
GTACACG1453.971045E-628.8105831
GGACCGT1055.8657234E-428.3650286
CATGGGA30200.028.0302754
CATGGGG20400.027.4690534
GTATAGG4250.026.6800561
GTATACG900.00938886126.5240291
GTGGTAT7200.026.5240291
Produced by FastQC (version 0.11.5)