FastQCFastQC Report
Fri 27 May 2016
SRR940059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823543
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48790.5924402247362919No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35240.42790722524506913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22280.27053839325936835No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10230.12421937895167588No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20300.073.140511
GTATCAA41100.059.9187771
ATCAACG51700.047.2819253
CAACGCA53350.046.0847635
TCAACGC53250.046.017614
AACGCAG55250.044.3706366
TATCAAC55200.044.2839782
TGGTATC8550.043.23422
GTGGTAT9700.041.1867751
ACGCAGA62700.038.998897
CGCAGAG63000.038.6243068
GTACCGT650.001938279536.6165436
TAACGCA1156.549126E-736.291264
GCAGAGT70000.034.5918929
GTACATG48600.033.6177751
TACATGG49000.032.7309072
ACATGGG47750.031.8396783
CATGGGG20700.028.5145634
GTACTTT44650.028.04843314-15
CAGAGTA69250.027.74985710-11
Produced by FastQC (version 0.11.5)