##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940059_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 823543 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.14040675471712 34.0 33.0 34.0 31.0 34.0 2 33.187247053280764 34.0 33.0 34.0 31.0 34.0 3 33.254007380306795 34.0 33.0 34.0 31.0 34.0 4 36.47593507564268 37.0 37.0 37.0 35.0 37.0 5 36.48306767224055 37.0 37.0 37.0 35.0 37.0 6 36.560704176952505 37.0 37.0 37.0 35.0 37.0 7 36.55624903617662 37.0 37.0 37.0 35.0 37.0 8 36.557279947738 37.0 37.0 37.0 35.0 37.0 9 38.434614828855324 39.0 39.0 39.0 37.0 39.0 10-11 38.419050978516 39.0 39.0 39.0 37.0 39.0 12-13 38.39688880847751 39.0 39.0 39.0 37.0 39.0 14-15 40.00580479707799 41.0 40.0 41.0 38.0 41.0 16-17 40.01124895724935 41.0 40.0 41.0 38.0 41.0 18-19 40.06149648530799 41.0 40.0 41.0 38.0 41.0 20-21 40.066079123980174 41.0 40.0 41.0 38.0 41.0 22-23 40.02281180217669 41.0 40.0 41.0 38.0 41.0 24-25 39.99075640251935 41.0 40.0 41.0 38.0 41.0 26-27 39.9044725047751 41.0 40.0 41.0 38.0 41.0 28-29 39.823943619215996 41.0 40.0 41.0 37.0 41.0 30-31 39.670232155455146 41.0 40.0 41.0 37.0 41.0 32-33 39.49687569440819 41.0 40.0 41.0 35.5 41.0 34-35 39.38902886673799 41.0 40.0 41.0 35.0 41.0 36-37 39.28957322204184 41.0 40.0 41.0 35.0 41.0 38-39 39.17578499240477 41.0 39.0 41.0 35.0 41.0 40-41 39.04812499165192 41.0 39.0 41.0 35.0 41.0 42-43 38.881083319268086 41.0 39.0 41.0 35.0 41.0 44-45 38.65913680281418 41.0 39.0 41.0 35.0 41.0 46-47 38.34112729997098 41.0 39.0 41.0 35.0 41.0 48-49 38.048285881854376 41.0 39.0 41.0 34.0 41.0 50-51 38.04878373078273 41.0 39.0 41.0 35.0 41.0 52-53 37.94615520986761 41.0 38.5 41.0 34.0 41.0 54-55 37.79082755848815 41.0 38.0 41.0 34.0 41.0 56-57 37.620330693114994 41.0 37.0 41.0 34.0 41.0 58-59 37.41540089588522 40.0 37.0 41.0 34.0 41.0 60-61 37.18963854953536 40.0 36.5 41.0 33.0 41.0 62-63 36.93378427113095 39.5 36.0 41.0 33.0 41.0 64-65 36.637779083787976 39.0 35.0 41.0 33.0 41.0 66-67 36.31799675305358 39.0 35.0 41.0 33.0 41.0 68-69 35.97098876439968 37.5 35.0 41.0 32.0 41.0 70-71 35.605798968602734 37.0 35.0 40.0 32.0 41.0 72-73 35.2370173749276 36.5 35.0 39.0 32.0 41.0 74-75 34.87266360105058 36.0 35.0 39.0 31.0 41.0 76-77 34.50449581867613 36.0 35.0 38.0 31.0 40.0 78-79 34.16122108985202 35.0 35.0 37.0 31.0 39.0 80-81 33.84912384659938 35.0 35.0 37.0 31.0 39.0 82-83 33.5703594104983 35.0 35.0 36.0 31.0 38.0 84-85 33.31594342979055 35.0 35.0 36.0 30.0 37.0 86-87 33.11763866610487 35.0 35.0 36.0 30.0 37.0 88-89 33.00659528403496 35.0 35.0 35.0 30.5 36.5 90-91 32.99911844311711 35.0 35.0 35.0 31.0 36.0 92-93 32.9131860752869 35.0 35.0 35.0 31.0 36.0 94-95 32.832873936151486 35.0 35.0 35.0 31.0 36.0 96-97 32.76817543224823 35.0 35.0 35.0 31.0 36.0 98-99 32.710950126465775 35.0 35.0 35.0 31.0 35.5 100-101 32.65833903026314 35.0 35.0 35.0 30.0 35.0 102-103 32.194964318803024 35.0 33.5 35.0 29.0 35.0 104-105 32.569684885913674 35.0 34.5 35.0 30.0 35.0 106-107 32.61091831270498 35.0 35.0 35.0 30.0 35.0 108-109 32.59929718302505 35.0 35.0 35.0 30.5 35.0 110-111 32.5625091828842 35.0 35.0 35.0 30.0 35.0 112-113 32.533835513142606 35.0 35.0 35.0 30.0 35.0 114-115 32.477390980191686 35.0 35.0 35.0 30.0 35.0 116-117 32.42311634000896 35.0 35.0 35.0 30.0 35.0 118-119 32.3650137272735 35.0 35.0 35.0 30.0 35.0 120-121 32.29075713107876 35.0 35.0 35.0 29.5 35.0 122-123 32.19072774584934 35.0 35.0 35.0 29.0 35.0 124-125 31.15184210174818 34.5 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10691840381320361 1101 2 0.08104919108365749 1101 3 0.11001493192283363 1101 4 -0.038679151021227653 1101 5 0.04654494904335138 1101 6 0.05275700756332924 1101 7 0.0541466732984901 1101 8 0.06616999936775159 1101 9 0.11747291617515287 1101 10-11 0.13480115142776583 1101 12-13 0.1479445350865376 1101 14-15 0.2092091303854673 1101 16-17 0.223690007735307 1101 18-19 0.09969440856074385 1101 20-21 0.08594485213902203 1101 22-23 0.09410112067502041 1101 24-25 0.08878321005853707 1101 26-27 0.18224102724295932 1101 28-29 0.15726053641215287 1101 30-31 0.2586823804854106 1101 32-33 0.1893124723045574 1101 34-35 0.23474664113267352 1101 36-37 0.16791520944118332 1101 38-39 0.22277867199289858 1101 40-41 0.21586201434802632 1101 42-43 0.30087594240698934 1101 44-45 0.3413724550033166 1101 46-47 0.39463446707973304 1101 48-49 0.4516152773449349 1101 50-51 0.38471032364537194 1101 52-53 0.29453559036767274 1101 54-55 0.22776463374908928 1101 56-57 0.2595481882851445 1101 58-59 0.3797525347752071 1101 60-61 0.4724946223963826 1101 62-63 0.3300644144682181 1101 64-65 0.320806850329717 1101 66-67 0.36942748579516405 1101 68-69 0.40988792650473016 1101 70-71 0.3670273150488015 1101 72-73 0.325347956320293 1101 74-75 0.29008841268828434 1101 76-77 0.3332739442514665 1101 78-79 0.2800211388574212 1101 80-81 0.20702152168507837 1101 82-83 0.20495051228915884 1101 84-85 0.3249144991864483 1101 86-87 0.22373874954426753 1101 88-89 0.28112911343775693 1101 90-91 0.33114005768250365 1101 92-93 0.260200269070026 1101 94-95 0.27981399800304274 1101 96-97 0.2508227457870831 1101 98-99 0.2780674650579229 1101 100-101 0.29001751320358693 1101 102-103 0.2956309101967278 1101 104-105 0.25939402677484935 1101 106-107 0.3071088831649007 1101 108-109 0.35452637549749966 1101 110-111 0.28133785321245597 1101 112-113 0.21131924849136396 1101 114-115 0.3226072158346085 1101 116-117 0.36233398049165544 1101 118-119 0.35197169148209184 1101 120-121 0.48037190519907114 1101 122-123 0.5029824271724905 1101 124-125 0.25072094528962907 1102 1 0.14980606238593452 1102 2 0.16241474513764587 1102 3 0.15806313706593755 1102 4 0.02821420381842188 1102 5 0.10572315597865867 1102 6 0.08058443392599912 1102 7 0.11644135823583923 1102 8 0.12948284357776174 1102 9 0.10381834438813797 1102 10-11 0.1388670072482725 1102 12-13 0.08943428325923009 1102 14-15 0.18062171739158828 1102 16-17 0.20941737161341933 1102 18-19 0.1438971491626262 1102 20-21 0.07889281557828554 1102 22-23 0.10176347154762055 1102 24-25 0.011641902920281666 1102 26-27 -0.011150625013513604 1102 28-29 0.007530091899894842 1102 30-31 -0.004950636550105969 1102 32-33 0.061160513728431454 1102 34-35 0.011400665574313962 1102 36-37 0.00384286297640557 1102 38-39 -0.03845038478086593 1102 40-41 0.013963743087529679 1102 42-43 0.02933257376261622 1102 44-45 -0.08570854726840338 1102 46-47 0.051748746012755475 1102 48-49 0.11661568906490771 1102 50-51 -0.10755612214563115 1102 52-53 -0.13357955559916945 1102 54-55 -0.16975196825332972 1102 56-57 -0.26583265268922673 1102 58-59 -0.20244808425788108 1102 60-61 -0.2907382997013528 1102 62-63 -0.2418475646454752 1102 64-65 -0.22951889282482796 1102 66-67 -0.3794780656431911 1102 68-69 -0.33960662668222596 1102 70-71 -0.4295835030145625 1102 72-73 -0.3513956777623761 1102 74-75 -0.2780764303010912 1102 76-77 -0.31139137584870014 1102 78-79 -0.18954936455501326 1102 80-81 -0.21996066779330192 1102 82-83 -0.18220721521034733 1102 84-85 -0.14623119640233995 1102 86-87 -0.19824322196470234 1102 88-89 -0.27741843537528155 1102 90-91 -0.20665971078555856 1102 92-93 -0.22423235658933294 1102 94-95 -0.23770686651202766 1102 96-97 -0.1683551705223536 1102 98-99 -0.15348094110140664 1102 100-101 -0.09086075815378081 1102 102-103 -0.13394414635422436 1102 104-105 -0.22272715452240277 1102 106-107 -0.24085649360731054 1102 108-109 -0.18548941517437356 1102 110-111 -0.07810579201085943 1102 112-113 -0.09312538970365125 1102 114-115 -0.23445378169023456 1102 116-117 -0.12727614491340944 1102 118-119 -0.05008448649718389 1102 120-121 -0.0545787610607249 1102 122-123 -0.09466931585613025 1102 124-125 -0.06818620459096891 1103 1 0.10730469402250264 1103 2 0.14120511185904405 1103 3 0.12138415512833944 1103 4 0.049649617064176255 1103 5 0.07610492937767077 1103 6 0.08906828723743843 1103 7 0.09832422632560167 1103 8 0.12636981675783687 1103 9 0.16423159014621547 1103 10-11 0.21324440188428184 1103 12-13 0.16531003585911463 1103 14-15 0.1964878914474113 1103 16-17 0.1862988713397442 1103 18-19 0.2049979975479559 1103 20-21 0.153444676552553 1103 22-23 0.24514059251095688 1103 24-25 0.19236166208830952 1103 26-27 0.22979422993996934 1103 28-29 0.21936985654139107 1103 30-31 0.17694249426543962 1103 32-33 0.2095663703239481 1103 34-35 0.18401971319335786 1103 36-37 0.03135723079250141 1103 38-39 0.007834508486780578 1103 40-41 0.1519529330163749 1103 42-43 0.12302441831652544 1103 44-45 0.13160809203099433 1103 46-47 0.012312511748930888 1103 48-49 -0.11297859117592424 1103 50-51 -0.1581239929721292 1103 52-53 -0.13940878384218536 1103 54-55 -0.14763921510608924 1103 56-57 -0.23368295372918624 1103 58-59 -0.2124610837105365 1103 60-61 -0.1947339209383543 1103 62-63 -0.18948713771607828 1103 64-65 -0.20881897492664336 1103 66-67 -0.08283055606464984 1103 68-69 -0.06835252159191185 1103 70-71 -0.20268489874526807 1103 72-73 -0.2262656832358303 1103 74-75 -0.2030593257581259 1103 76-77 -0.26395705729368757 1103 78-79 -0.20560710949207106 1103 80-81 -0.14912049264277982 1103 82-83 -0.200399607109631 1103 84-85 -0.17181959268039293 1103 86-87 -0.24342658266530748 1103 88-89 -0.28029541950226644 1103 90-91 -0.21910839715665986 1103 92-93 -0.22307951039338292 1103 94-95 -0.2336051971086306 1103 96-97 -0.18156329860116927 1103 98-99 -0.23442990114519802 1103 100-101 -0.35027167769401046 1103 102-103 -0.3230040806727814 1103 104-105 -0.24565408391485022 1103 106-107 -0.20264494461990523 1103 108-109 -0.2020431918574559 1103 110-111 -0.22561537055492664 1103 112-113 -0.2988998834091774 1103 114-115 -0.2353055879299717 1103 116-117 -0.21161864628176374 1103 118-119 -0.32177988879603703 1103 120-121 -0.27075623779853686 1103 122-123 -0.3326071921560718 1103 124-125 -0.39993223633700126 1104 1 0.07605797900095013 1104 2 0.09739663924262487 1104 3 0.15885261933521377 1104 4 0.055989625473721105 1104 5 0.12193037876271973 1104 6 0.07650652964257176 1104 7 0.11130646116214393 1104 8 0.07350137357482822 1104 9 0.1668464329928412 1104 10-11 0.1953149895012274 1104 12-13 0.1931272333782985 1104 14-15 0.18018782837582847 1104 16-17 0.18228462767318376 1104 18-19 0.23988775367115522 1104 20-21 0.20435198760059592 1104 22-23 0.22083175197694516 1104 24-25 0.21694644081216552 1104 26-27 0.3225915861893114 1104 28-29 0.33183766375257306 1104 30-31 0.3137914115365419 1104 32-33 0.35911829926329375 1104 34-35 0.31130605819493695 1104 36-37 0.29626774797950617 1104 38-39 0.3272723829743285 1104 40-41 0.4263964576668542 1104 42-43 0.4279623979233875 1104 44-45 0.4447256679796894 1104 46-47 0.29206127659703185 1104 48-49 0.17440774856968488 1104 50-51 0.059742347824673914 1104 52-53 0.04594284025115769 1104 54-55 0.07600211780677313 1104 56-57 -0.045815036142727195 1104 58-59 -0.07041533053067184 1104 60-61 -0.14043979710780974 1104 62-63 -0.08922302544865346 1104 64-65 -0.009266388991605368 1104 66-67 -0.018668356968667865 1104 68-69 -0.11590258466349468 1104 70-71 -0.09021052157718401 1104 72-73 -0.031420471420425145 1104 74-75 -0.08716200104456817 1104 76-77 -0.07097771323818591 1104 78-79 -0.19133059314601297 1104 80-81 -0.06474910611907347 1104 82-83 -0.001007985118675947 1104 84-85 -0.006440637566328178 1104 86-87 -0.0773643520335412 1104 88-89 0.0025248001970936684 1104 90-91 0.011684333843021477 1104 92-93 -0.05627815961024396 1104 94-95 8.864196263616009E-4 1104 96-97 0.015072226469783345 1104 98-99 -0.002034100661646221 1104 100-101 -0.07390972562841824 1104 102-103 -0.15813101224011206 1104 104-105 -0.07404023970166662 1104 106-107 -0.018153984880598273 1104 108-109 0.020865590860921657 1104 110-111 0.010482600642383488 1104 112-113 -0.01599753924855918 1104 114-115 0.005046430360792442 1104 116-117 0.004799019018349782 1104 118-119 0.03431834519955146 1104 120-121 0.07213966780431491 1104 122-123 0.14000265752331842 1104 124-125 0.15262741887166342 1105 1 0.1661443218276446 1105 2 0.11944813320551617 1105 3 0.1661638486149286 1105 4 0.09244518356663889 1105 5 0.11034822439683012 1105 6 0.16501245800919406 1105 7 0.18288908675253168 1105 8 0.1245567352033774 1105 9 0.18126771494095806 1105 10-11 0.1096934987644147 1105 12-13 0.18001994281040368 1105 14-15 0.23168904087269482 1105 16-17 0.2649455599002266 1105 18-19 0.2539683040613667 1105 20-21 0.3305622189718207 1105 22-23 0.23754617543377066 1105 24-25 0.2801421765929675 1105 26-27 0.28396431751522755 1105 28-29 0.2809975248500933 1105 30-31 0.18941170608810154 1105 32-33 0.2312207217196729 1105 34-35 0.15227368144732623 1105 36-37 0.22486092537378255 1105 38-39 0.28263609578837645 1105 40-41 0.22904310816689843 1105 42-43 0.1661278665923902 1105 44-45 0.12952133778906472 1105 46-47 0.054252851103065325 1105 48-49 0.10471092716014851 1105 50-51 0.09882045147231366 1105 52-53 -0.07112744831947282 1105 54-55 -0.032559850026721904 1105 56-57 -0.031609883121632265 1105 58-59 -0.04136434041551951 1105 60-61 0.13743630347324398 1105 62-63 -0.12575561062248397 1105 64-65 -0.17384018455991423 1105 66-67 -0.16110094467986613 1105 68-69 -0.08130066061763586 1105 70-71 -0.1321970771798604 1105 72-73 -0.1616447199079687 1105 74-75 -0.16654112652440745 1105 76-77 -0.08288836544913636 1105 78-79 -0.0982932069195428 1105 80-81 -0.1330422222541614 1105 82-83 -0.06441849052507109 1105 84-85 0.035761140762417654 1105 86-87 -0.013609943365906929 1105 88-89 0.006462243332983064 1105 90-91 -0.06210579726612764 1105 92-93 -0.09809661493634536 1105 94-95 -0.011297452171575628 1105 96-97 -0.0642774474791139 1105 98-99 -0.08228499145717905 1105 100-101 -0.05027509860260437 1105 102-103 -0.05087080207398742 1105 104-105 -0.09715244187872685 1105 106-107 -0.061190374286013594 1105 108-109 -0.036693574999219436 1105 110-111 -0.021318709361715094 1105 112-113 0.06036393706153831 1105 114-115 -0.026407804678740376 1105 116-117 -0.0029603266320705757 1105 118-119 0.001803855046325964 1105 120-121 -0.007739680600948873 1105 122-123 -0.03598020802909474 1105 124-125 -0.14767443666540458 1106 1 0.03398981768436471 1106 2 0.12849562496351297 1106 3 0.10136477528337906 1106 4 -0.011707894932868612 1106 5 0.021511906121858715 1106 6 0.034989291297975456 1106 7 0.022849881548928863 1106 8 0.09536667906865404 1106 9 0.10158670273388282 1106 10-11 0.07510025275176702 1106 12-13 0.13794384907432544 1106 14-15 0.134763085245865 1106 16-17 0.11821563029138105 1106 18-19 0.09122215775467879 1106 20-21 0.11810922066477758 1106 22-23 0.1991789830609676 1106 24-25 0.13543844318834886 1106 26-27 0.13989989623749466 1106 28-29 0.09894949847567602 1106 30-31 0.11871759577891794 1106 32-33 0.17038493806111177 1106 34-35 0.06515481839822712 1106 36-37 0.1437008077581723 1106 38-39 0.09419940328191245 1106 40-41 0.010164911606260318 1106 42-43 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2216 100-101 -0.13349856333878307 2216 102-103 -0.2442979631892932 2216 104-105 -0.2067029195074852 2216 106-107 -0.1854467636443502 2216 108-109 -0.13829406081818263 2216 110-111 -0.18995901555550176 2216 112-113 -0.06430945356327555 2216 114-115 -0.16314926189591006 2216 116-117 -0.2577640813414348 2216 118-119 -0.13606125827813287 2216 120-121 -0.2344590242659308 2216 122-123 -0.2586224279340783 2216 124-125 -0.3194375080320526 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 3.0 4 11.0 5 32.0 6 89.0 7 231.0 8 369.0 9 634.0 10 1166.0 11 1781.0 12 2332.0 13 3834.0 14 7107.0 15 7489.0 16 3232.0 17 2402.0 18 2226.0 19 1804.0 20 1575.0 21 1484.0 22 1472.0 23 1639.0 24 1946.0 25 2431.0 26 2892.0 27 3668.0 28 4387.0 29 5474.0 30 6743.0 31 8412.0 32 10831.0 33 14385.0 34 21628.0 35 39665.0 36 95747.0 37 324699.0 38 237704.0 39 2012.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47545449356258 18.889977884086377 14.0616403668254 22.572927255525638 2 15.425602964855484 19.94453039049731 41.31769465971434 23.31217198493286 3 19.280133860810984 25.716235480400513 28.975814226839674 26.027816431948825 4 12.325673866492538 16.762303603804078 37.70320569176574 33.20881683793765 5 13.232289366209674 38.12739460110835 33.5821167484202 15.058199284261779 6 31.004929494505173 36.84332611539073 17.52405542311191 14.627688966992183 7 28.321168415006863 33.26420114068117 20.500204603766896 17.91442584054506 8 26.345072449161734 35.316674417729246 19.336938083378765 19.00131504973025 9 27.34599164828066 16.47260677341681 19.985841662184004 36.195559916118526 10-11 25.188180823587842 27.163548230025636 26.58525420044855 21.06301674593798 12-13 26.1782323448806 24.334248484899998 27.670261297831445 21.817257872387962 14-15 23.05143750842397 25.27002233034584 26.481373771618482 25.197166389611713 16-17 21.83231476680635 28.921137086952353 26.889124186593776 22.357423959647523 18-19 21.58988662401356 26.69714878275937 30.36714537067281 21.34581922255426 20-21 22.685639972654737 25.755607175338753 30.47424360355197 21.084509248454545 22-23 22.22397616153619 25.7797710623489 30.357188392105815 21.639064384009092 24-25 21.933463097858883 25.96057522193741 30.453722513578523 21.65223916662518 26-27 21.685570759511037 26.25224183800967 30.45256896118357 21.60961844129572 28-29 21.51461521415106 26.085903277608622 30.682205229238317 21.717276279001997 30-31 21.80056171930306 25.838237954788028 30.600587947441728 21.760612378467183 32-33 21.618799272654414 26.078678392432693 30.43850195952243 21.864020375390464 34-35 22.06783414972958 26.135831012048016 30.17838885394481 21.617945984277593 36-37 22.05343785821142 25.96140302306153 30.126187561928074 21.858971556798974 38-39 21.78059602536423 26.151092502689664 30.192757449102757 21.87555402284335 40-41 21.98707292758241 25.91443312613913 30.306614226579548 21.791879719698912 42-43 21.93221903137086 25.946013223481696 29.88191151560043 22.239856229547016 44-45 21.936146237332096 26.21730873378035 29.67003344107983 22.176511587807727 46-47 22.17661294337501 26.43104593456792 29.071776632857382 22.320564489199686 48-49 22.32511912105419 26.613234215549607 28.76050960982694 22.30113705356926 50-51 22.435410553490257 26.74036174825765 28.40648502146556 22.417742676786535 52-53 22.656254743225308 26.755797815050336 28.358871364336775 22.22907607738758 54-55 22.322574534663033 26.76769761870358 28.426141682947947 22.483586163685445 56-57 22.307606374000798 26.98695741397127 28.378219209187733 22.327217002840204 58-59 22.367464005605132 26.56512019583962 28.61361624516867 22.453799553386574 60-61 22.43802009148389 26.72096928720525 28.704982071149548 22.136028550161317 62-63 22.24190008408795 26.70530057647298 28.859530625316847 22.193268714122222 64-65 22.207043689282074 26.407004467952955 28.736787846002247 22.649163996762724 66-67 22.243235891968556 26.894812382670867 28.366025611447192 22.495926113913384 68-69 22.260309943384485 26.632302718455442 28.6705826996342 22.436804638525874 70-71 22.17126960458124 26.648873389255062 28.705259783469252 22.47459722269444 72-73 22.314135388194668 26.641413988097767 28.356382119695024 22.68806850401254 74-75 22.220448040838285 26.958524226480527 28.144561108675823 22.67646662400536 76-77 22.33143577401234 26.85783119655525 28.156716218546375 22.65401681088603 78-79 22.348101938284533 26.78949046036155 28.220208551523157 22.64219904983076 80-81 22.172794863170473 27.144059265879257 28.077708146387014 22.60543772456326 82-83 22.550735055728723 26.906913178789694 28.003759366541882 22.538592398939702 84-85 22.48848418689283 26.842180995128967 27.80357759748598 22.865757220492227 86-87 22.284062187515747 26.807817213825896 28.186436263381992 22.721684335276365 88-89 22.29586068972719 26.8355750701542 28.07637245414022 22.79219178597839 90-91 22.35611541366439 26.949870194841306 28.18708699738447 22.506927394109834 92-93 22.462020620759915 26.63316491386565 28.052162678391674 22.852651786982765 94-95 22.275387369928033 26.61473198754879 28.080426503079103 23.029454139444066 96-97 22.358706098850025 26.624677232024414 28.015197795372544 23.00141887375302 98-99 22.15804153517181 26.580032858029274 27.978381213852828 23.283544392946087 100-101 22.209593820826054 26.489164770888618 28.190963285958613 23.11027812232671 102-103 22.50890360309055 26.436628081350943 27.961502920916093 23.092965394642416 104-105 22.31941744389789 26.37470053172694 28.01517346392356 23.29070856045161 106-107 22.242743374490995 26.448547460628834 28.11033580012155 23.198373364758623 108-109 22.394365769621906 26.422779776270055 27.713370672252324 23.46948378185571 110-111 22.127806319767153 26.66011367955286 27.91967146827792 23.292408532402074 112-113 22.097429212735484 26.60667373775335 28.01285666842533 23.283040381085833 114-115 21.88798336914464 26.71009356677569 27.878297013039603 23.52362605104007 116-117 21.82087750762868 26.621268989735736 27.97639936032018 23.5814541423154 118-119 21.86144952103174 26.672381824341475 27.676096272073885 23.7900723825529 120-121 21.829258149103598 26.7267648321305 27.831141478601868 23.612835540164035 122-123 21.84670942109101 26.69704768617937 27.73318373534756 23.72305915738206 124-125 21.83293859220398 26.743838000911925 27.62030294990319 23.802920456980907 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 181.0 1 129.0 2 203.0 3 341.5 4 222.0 5 143.5 6 389.0 7 1059.5 8 1971.0 9 2157.0 10 1491.5 11 920.0 12 755.5 13 814.5 14 902.0 15 949.0 16 1061.5 17 1175.5 18 1267.5 19 1371.0 20 1462.0 21 1607.5 22 1853.0 23 2211.5 24 2618.5 25 3147.5 26 4150.0 27 5252.5 28 6171.0 29 7572.5 30 9385.5 31 10966.5 32 12641.5 33 14532.0 34 16601.0 35 18192.0 36 20418.0 37 23140.5 38 24126.5 39 24625.0 40 24746.0 41 25460.0 42 26801.0 43 27985.5 44 29260.5 45 30431.0 46 30175.5 47 29387.5 48 30018.5 49 30318.5 50 30158.5 51 29485.5 52 28290.5 53 27943.0 54 27359.0 55 25228.5 56 22650.5 57 20300.5 58 18588.5 59 16720.5 60 14058.0 61 11339.0 62 9170.0 63 7289.0 64 5813.0 65 4555.5 66 3221.5 67 2529.0 68 1823.0 69 1078.0 70 803.5 71 604.0 72 485.5 73 423.0 74 321.5 75 269.5 76 178.5 77 85.0 78 51.5 79 20.0 80 9.0 81 5.5 82 4.0 83 2.5 84 0.5 85 0.0 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018699691455090994 2 0.003521370468815836 3 1.2142656789020124E-4 4 0.14109767188841385 5 0.040920753378997816 6 0.016028306961506564 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 6.071328394510062E-5 30-31 0.0 32-33 6.071328394510062E-5 34-35 6.071328394510062E-4 36-37 8.499859752314087E-4 38-39 0.002549957925694226 40-41 0.0 42-43 9.714125431216099E-4 44-45 6.071328394510062E-4 46-47 2.4285313578040248E-4 48-49 0.0013356922467922137 50-51 0.001760685234407918 52-53 0.0 54-55 0.0 56-57 0.0012142656789020125 58-59 0.0014571188146824147 60-61 0.0012142656789020125 62-63 6.071328394510062E-5 64-65 0.0013964055307373142 66-67 6.071328394510062E-4 68-69 6.678461233961069E-4 70-71 0.0013356922467922137 72-73 0.0 74-75 2.4285313578040248E-4 76-77 0.003521370468815836 78-79 6.678461233961069E-4 80-81 0.0 82-83 0.0 84-85 1.8213985183530184E-4 86-87 1.8213985183530184E-4 88-89 0.0 90-91 1.2142656789020124E-4 92-93 4.8570627156080496E-4 94-95 0.0010321258270667105 96-97 3.035664197255031E-4 98-99 0.0 100-101 3.035664197255031E-4 102-103 0.0 104-105 0.0 106-107 3.035664197255031E-4 108-109 6.678461233961069E-4 110-111 0.0 112-113 1.8213985183530184E-4 114-115 0.0016392586665177167 116-117 7.285594073412074E-4 118-119 2.4285313578040248E-4 120-121 1.8213985183530184E-4 122-123 3.035664197255031E-4 124-125 9.106992591765094E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 823543.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.150410579505326 #Duplication Level Percentage of deduplicated Percentage of total 1 78.22764284762609 45.489695502563606 2 12.588678507747272 14.640736477577967 3 3.9411062560418384 6.875308407788698 4 1.6898414106293245 3.9305988736938273 5 0.9073631604036616 2.638177016109524 6 0.5580214729981842 1.9469506660214853 7 0.3649013536692414 1.4853414475818572 8 0.24391668957535645 1.134708451680058 9 0.2076599966934065 1.0867962661794277 >10 1.1525453149777218 12.689986742760539 >50 0.08299438304389736 3.265471912299215 >100 0.034074336543834106 3.246638663842958 >500 4.180900167343358E-4 0.15292314757212852 >1k 8.361800334686716E-4 1.4166664243287643 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4879 0.5924402247362919 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3524 0.42790722524506913 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2228 0.27053839325936835 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1023 0.12421937895167588 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.8213985183530187E-4 0.0 14-15 0.0 0.0 0.0 2.4285313578040248E-4 0.0 16-17 0.0 0.0 0.0 3.6427970367060373E-4 0.0 18-19 0.0 0.0 0.0 4.8570627156080496E-4 0.0 20-21 0.0 0.0 0.0 4.8570627156080496E-4 0.0 22-23 0.0 0.0 0.0 6.678461233961069E-4 0.0 24-25 0.0 0.0 0.0 7.892726912863081E-4 0.0 26-27 0.0 0.0 0.0 9.106992591765093E-4 0.0 28-29 0.0 0.0 0.0 0.0018213985183530186 0.0 30-31 0.0 0.0 0.0 0.003946363456431541 0.0 32-33 0.0 0.0 0.0 0.00868199960414939 0.0 34-35 0.0 0.0 0.0 0.01548188740600066 0.0 36-37 0.0 0.0 0.0 0.027988823898691388 0.0 38-39 0.0 6.071328394510062E-5 0.0 0.050452738958378616 0.0 40-41 0.0 1.2142656789020124E-4 0.0 0.0782594230052347 0.0 42-43 0.0 1.2142656789020124E-4 0.0 0.10266616315116514 0.0 44-45 0.0 1.2142656789020124E-4 0.0 0.13199067929664876 0.0 46-47 0.0 1.2142656789020124E-4 0.0 0.1672043839848071 0.0 48-49 0.0 1.2142656789020124E-4 0.0 0.20484662003076948 0.0 50-51 0.0 1.2142656789020124E-4 0.0 0.24601022654554772 0.0 52-53 0.0 1.2142656789020124E-4 0.0 0.28917737143051425 0.0 54-55 0.0 1.2142656789020124E-4 0.0 0.33653373290769273 0.0 56-57 0.0 1.2142656789020124E-4 0.0 0.395850611322056 0.0 58-59 0.0 1.2142656789020124E-4 0.0 0.4625137970937765 0.0 60-61 0.0 1.2142656789020124E-4 0.0 0.5360375839512934 0.0 62-63 0.0 1.2142656789020124E-4 0.0 0.6109577763395475 0.0 64-65 0.0 1.2142656789020124E-4 0.0 0.6842994233452291 0.0 66-67 0.0 1.2142656789020124E-4 0.0 0.7647445245724874 0.0 68-69 0.0 1.2142656789020124E-4 0.0 0.8612786460451973 0.0 70-71 0.0 1.2142656789020124E-4 0.0 0.9738410744794139 0.0 72-73 0.0 1.2142656789020124E-4 0.0 1.089864160098501 0.0 74-75 0.0 1.2142656789020124E-4 0.0 1.2030944346561139 0.0 76-77 0.0 1.2142656789020124E-4 0.0 1.3315030302000017 0.0 78-79 0.0 1.2142656789020124E-4 0.0 1.4877790230746908 0.0 80-81 0.0 1.2142656789020124E-4 0.0 1.6695545952063218 0.0 82-83 0.0 1.2142656789020124E-4 0.0 1.8503587547948315 0.0 84-85 0.0 1.2142656789020124E-4 0.0 2.0300700752723295 0.0 86-87 0.0 1.2142656789020124E-4 0.0 2.2152455913048863 0.0 88-89 0.0 1.2142656789020124E-4 0.0 2.4341169799269737 0.0 90-91 0.0 1.2142656789020124E-4 0.0 2.6882627865211655 0.0 92-93 0.0 1.2142656789020124E-4 0.0 2.961533277558063 0.0 94-95 0.0 1.2142656789020124E-4 0.0 3.231707391113761 0.0 96-97 0.0 1.2142656789020124E-4 0.0 3.4950816168676075 0.0 98-99 0.0 1.2142656789020124E-4 0.0 3.7985266100252204 0.0 100-101 0.0 1.2142656789020124E-4 0.0 4.1447137550801845 0.0 102-103 0.0 1.2142656789020124E-4 0.0 4.522046814798984 0.0 104-105 0.0 1.2142656789020124E-4 0.0 4.896101357184749 0.0 106-107 0.0 1.2142656789020124E-4 0.0 5.266816668953534 0.0 108-109 0.0 1.2142656789020124E-4 0.0 5.658478063683377 0.0 110-111 0.0 1.2142656789020124E-4 0.0 6.093488743150024 0.0 112-113 0.0 1.2142656789020124E-4 0.0 6.571909420637416 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2030 0.0 73.14051 1 GTATCAA 4110 0.0 59.918777 1 ATCAACG 5170 0.0 47.281925 3 CAACGCA 5335 0.0 46.084763 5 TCAACGC 5325 0.0 46.01761 4 AACGCAG 5525 0.0 44.370636 6 TATCAAC 5520 0.0 44.283978 2 TGGTATC 855 0.0 43.2342 2 GTGGTAT 970 0.0 41.186775 1 ACGCAGA 6270 0.0 38.99889 7 CGCAGAG 6300 0.0 38.624306 8 GTACCGT 65 0.0019382795 36.616543 6 TAACGCA 115 6.549126E-7 36.29126 4 GCAGAGT 7000 0.0 34.591892 9 GTACATG 4860 0.0 33.617775 1 TACATGG 4900 0.0 32.730907 2 ACATGGG 4775 0.0 31.839678 3 CATGGGG 2070 0.0 28.514563 4 GTACTTT 4465 0.0 28.048433 14-15 CAGAGTA 6925 0.0 27.749857 10-11 >>END_MODULE