FastQCFastQC Report
Fri 27 May 2016
SRR940058_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940058_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864620
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19200.22206287155050772No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.047.524151
GTATCAA17450.044.4484331
TCAACGC19250.038.4102864
ATCAACG19850.037.843483
CAACGCA19800.037.323855
AACGCAG20800.035.815966
TATCAAC22900.033.6095542
GTACATG41050.031.394181
TACATGG41700.030.0463542
CGCAGAG25300.029.209978
ACGCAGA25600.028.8676647
ACATGGG42000.027.5380693
GTGTACG1250.001607320323.8653891
GTATAGC4550.023.6031341
GTCTATG5850.023.4574341
CATGGGG20350.023.4413264
GCAGAGT30800.023.0184049
GTGGTAT6250.021.9561581
GAGTACA33000.021.6958081
CATGGGA24850.020.8761044
Produced by FastQC (version 0.11.5)