FastQCFastQC Report
Fri 27 May 2016
SRR940058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864620
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56500.6534662626356087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41000.47419675695681335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26820.3101940736971155No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.1379796904998728No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.10432328653049894No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22950.072.19971
TATCACG701.72804E-1068.1106342
GTATCAA42400.056.3704871
GTGGTAT8300.051.7044221
ATCAACG53800.044.3073233
TCAACGC54850.043.459144
ATCACGC1109.493306E-943.3406143
CAACGCA55050.043.148015
TATCAAC55400.043.137762
AACGCAG56950.041.8933036
TGGTATC9900.040.333192
ACGCAGA65300.036.4431277
CGCAGAG65650.036.158228
GCAGAGT71900.032.849639
GGTAGCG750.003912076431.7298167
ATAACGC1352.3013472E-630.9002553
GTACATG42050.030.1916521
TACATGG43050.029.0716112
GAGTACT49800.028.67152212-13
ACATGGG41650.028.4731713
Produced by FastQC (version 0.11.5)