FastQCFastQC Report
Fri 27 May 2016
SRR940057_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940057_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1894215
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26800.051.869771
CAACGCA30150.044.2715875
ATCAACG30400.043.53163
TCAACGC30700.043.495714
AACGCAG31900.041.6560976
GGTATCA18900.041.352591
TATCAAC33900.039.9491772
ACGCAGA38350.034.0285267
CGCAGAG40350.031.7511378
GTACATG66900.030.8562531
TACATGG69100.029.5277082
ACATGGG70150.028.0421663
GCAGAGT48650.025.220499
CATGGGG50300.023.3472084
CCGATCG1653.1937863E-421.6589019
GGACCGA5000.021.4519566
AGTACAT50500.020.792322
CAGAGTA47200.020.29522710-11
ACCGAGT5400.019.862928
GAGTACA56250.019.834091
Produced by FastQC (version 0.11.5)