FastQCFastQC Report
Fri 27 May 2016
SRR940057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1894215
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35180.18572337353468324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27890.14723777395913348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27300.056.3253751
GTATCAA45350.055.8545651
ATCAACG54700.045.752743
TCAACGC55550.045.052654
CAACGCA56050.044.6035845
TATCAAC57600.043.449222
AACGCAG59500.042.105146
ACGCAGA69200.036.1085437
CGCAGAG73800.033.7772648
GTACATG64600.030.9994351
GCAGAGT83200.029.6750559
TACATGG66900.029.1257252
ACATGGG66100.028.6669033
CAGAGTA78500.024.93406110-11
CATGGGG48300.024.920654
GAGTACT48650.024.57989312-13
GTGGTAT13100.023.6580561
GTACTTT50600.023.16234214-15
GGACGAT7050.022.7846037
AGAGTAC80550.022.6007410-11
Produced by FastQC (version 0.11.5)