FastQCFastQC Report
Fri 27 May 2016
SRR940056_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940056_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326508
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22300.057.255361
GGTATCA13300.048.448391
ATCAACG26900.046.1005673
TCAACGC27050.045.850134
CAACGCA27700.044.745465
AACGCAG29400.041.955466
TATCAAC31000.040.229172
GTACATG58450.035.6244471
ACGCAGA36000.033.767057
TACATGG60950.033.1881072
CGCAGAG37050.032.6492588
ACATGGG62750.031.354183
CATGGGA31100.029.7177414
GCAGAGT42900.027.628959
TATATCG900.009460364526.483965
CATGGGG33350.023.2429894
ATGGGAT15350.022.515685
GAGTACA44850.021.550621
AGAGTAC40300.021.40863610-11
ATGGGAG18100.021.3993345
Produced by FastQC (version 0.11.5)