FastQCFastQC Report
Fri 27 May 2016
SRR940056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326508
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46290.3489613330639544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34920.26324756428155727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20520.15469186767060583No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28500.069.252671
GTATCAA48250.052.6460341
ATCAACG59100.042.060143
TCAACGC60150.041.3259244
TATCAAC60500.041.1853752
CAACGCA61000.040.7892535
AACGCAG64500.038.750236
ACGCAGA74600.033.335547
CGCAGAG76850.032.1273048
GTACATG59250.030.896081
TACATGG59700.029.954982
GCAGAGT85100.028.733099
ACATGGG62900.027.483343
GAGTACT50900.027.23379712-13
TAGCGCG900.00944374126.4935194
TACGCTA1150.001005528425.8667729
GTACTTT54300.025.58333214-15
CAGAGTA85650.025.1797110-11
TGGTATC13150.024.0254922
AGAGTAC80950.023.88562410-11
Produced by FastQC (version 0.11.5)