FastQCFastQC Report
Fri 27 May 2016
SRR940055_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940055_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090086
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17900.051.991761
ATCAACG19650.045.5042533
TCAACGC20100.044.4916464
CAACGCA20300.044.3184555
AACGCAG21300.042.2377746
GGTATCA12000.041.262911
TATCAAC22800.040.5601732
GTACATG48500.034.5642851
ACGCAGA25800.034.1779257
TACATGG48300.033.7223972
ACATGGG49100.031.4442753
CGCAGAG27950.031.3356848
TATTCCG1306.319552E-527.4986635
CATGGGG30900.026.6258744
GCAGAGT34850.024.6095549
CATGGGA23150.023.4354694
GAGTACA36900.022.4725021
GTATAGC5700.021.9789751
AGTACAT34700.021.6642682
CGCGGAA2500.021.41106882-83
Produced by FastQC (version 0.11.5)