FastQCFastQC Report
Fri 27 May 2016
SRR940055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940055_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090086
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39340.3608889573850137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29450.2701621706911198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17460.16017084890549918No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19650.071.3145451
GTATCAA36550.058.7337721
ATCAACG45500.046.7702753
TCAACGC45750.046.5146984
CAACGCA47000.045.346085
TATCAAC46900.045.2470472
AACGCAG49850.042.7320026
GTGGTAT8450.042.341611
ACGCAGA56550.037.5587587
TGGTATC9750.037.2939452
CGCAGAG57850.036.714748
GTACATG46550.032.2814981
GCAGAGT66950.031.3689339
TACATGG48300.030.8536112
ACATGGG47650.030.52393
ATAACGC800.00531424229.8045863
TAACCCG1107.726239E-427.0602555
CAGAGTA64100.026.54505510-11
GTACTTT41100.025.91115814-15
AGAGTAC61850.025.7311810-11
Produced by FastQC (version 0.11.5)