FastQCFastQC Report
Fri 27 May 2016
SRR940054_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940054_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244287
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20050.054.767041
ATCAACG21650.047.6490063
TCAACGC22000.046.8947334
GGTATCA11550.045.9858131
CAACGCA22750.045.3067555
TATCAAC24400.043.289422
AACGCAG24250.042.2585836
ACGCAGA29400.034.8561447
GTACATG53050.033.8607751
CGCAGAG31350.032.3079648
TACATGG56050.032.1536062
GTACTAT3350.032.0659071
ACATGGG55050.031.5210953
GTGGTAT7400.028.2261831
GCAGAGT36350.027.0346289
CATGGGG34800.024.8478854
CATGGGA24750.023.613024
GTATTAG4101.8189894E-1221.8334941
TGGTATC9200.021.4054532
ATGGGGA19350.021.2454725
Produced by FastQC (version 0.11.5)