FastQCFastQC Report
Fri 27 May 2016
SRR940054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940054_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244287
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43360.348472659442717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33630.2702752660760741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19430.15615368480101455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.070.525651
GTATCAA43150.056.3674771
GTGGTAT9550.046.8173831
ATCAACG53250.044.999043
CAACGCA53650.044.5988925
TCAACGC53750.044.580444
TATCAAC54200.044.430262
AACGCAG56450.042.4785046
TAACGCA1002.1945925E-741.7248544
TGGTATC11950.037.909012
ACGCAGA66250.036.010927
CGCAGAG67000.035.430228
ATAACGC1209.159685E-734.770713
GCAGAGT77050.030.6544579
GTACATG52150.029.9499571
TACATGG54250.028.7871282
ACATGGG52950.028.5933153
GTACGCT850.007147663728.0503223
GTATAGG3900.027.5142151
GAGTACT47500.026.80377212-13
Produced by FastQC (version 0.11.5)