FastQCFastQC Report
Fri 27 May 2016
SRR940053_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940053_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379427
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.054.7569661
GTATCAA24800.049.797331
TCAACGC28750.042.0903364
ATCAACG29350.041.422453
CAACGCA29950.040.374565
AACGCAG31750.038.085616
TATCAAC33850.036.118082
GTACATG68400.032.3597561
TACATGG68000.031.6612282
ACGCAGA38800.030.7048447
ACATGGG67950.029.9951043
CGCAGAG40550.029.2328248
GTGGTAT10300.025.4860691
GGTATAC2401.2803866E-824.8314723
GCAGAGT48000.024.1913459
CATGGGG37300.024.1319454
GTGTAGC7750.023.8642271
GAGTACA52450.023.5457381
CATGGGA40200.023.2807964
AGTACAT52800.021.9127672
Produced by FastQC (version 0.11.5)