FastQCFastQC Report
Fri 27 May 2016
SRR940053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940053_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379427
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46520.3372414778020149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35070.25423599799047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21220.15383198965947456No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.076.1827241
GTATCAA49450.057.3955881
ATCAACG63200.044.610793
TCAACGC64050.044.0187644
TATCAAC64750.043.7349432
CAACGCA65550.043.221495
AACGCAG68750.041.282826
GTGGTAT13050.037.9232371
TTACGCG401.19830744E-437.1853880-81
ACGCAGA79800.035.5624737
CGCAGAG80400.035.1490868
TGGTATC12800.034.0006832
GTACATG68900.032.106471
GCAGAGT89100.031.5167039
TACATGG71450.030.789232
ACATGGG70450.030.628343
GAGTACT52050.027.8326612-13
CAGAGTA87400.026.58190210-11
GTACTTT53950.026.57676114-15
AGAGTAC84300.025.40688510-11
Produced by FastQC (version 0.11.5)