FastQCFastQC Report
Fri 27 May 2016
SRR940052_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940052_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302706
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14250.059.0512921
GTATCAA23550.043.080741
CAACGCA25800.037.4083145
ATCAACG25950.037.223573
TCAACGC26250.036.8009954
AACGCAG27600.034.9686366
TATCAAC30250.032.9432222
GTACATG57750.031.6223831
TACATGG59450.030.2127062
ACATGGG60650.028.5106853
ACGCAGA34550.027.9344257
CGCAGAG36300.026.259488
CATGGGG34150.026.0177334
GCAGAGT41750.021.823489
CAGAGTA39250.021.22072410-11
GTGGTAT9200.020.7580871
GAGTACA45600.020.5475461
GTGTAGC8800.020.3452841
CATGGGA34650.019.7910354
AGTACAT45450.019.4313412
Produced by FastQC (version 0.11.5)