FastQCFastQC Report
Fri 27 May 2016
SRR940052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302706
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41450.31818384194131294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33950.2606113735562744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18640.14308677475961573No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23200.067.856641
GTATCAA46700.060.525431
ATCAACG57500.048.8366553
TCAACGC58600.048.021674
CAACGCA59150.047.599045
TATCAAC60300.046.6678242
AACGCAG61150.046.0298736
ACGCAGA73750.038.0748677
CGCAGAG74600.037.401798
GCAGAGT80850.034.289759
GTGGTAT12550.033.73581
TGGTATC12500.031.4794272
GTACATG58950.030.6503351
TACATGG59600.029.409932
ACATGGG58850.028.9742683
GAGTACT49100.028.77667212-13
GTACTTT52000.026.88579814-15
CAGAGTA78800.026.8015910-11
AGTACTT51550.026.77427712-13
AGAGTAC77050.026.29074310-11
Produced by FastQC (version 0.11.5)