FastQCFastQC Report
Fri 27 May 2016
SRR940051_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940051_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644196
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42460.6591161696129749No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8020.12449627132115071No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16700.056.1009671
ATCAACG20400.044.7104343
TCAACGC20800.044.1372264
CAACGCA21000.043.6862755
GGTATCA9750.043.4551121
AACGCAG22100.041.2422876
TATCAAC22650.041.1033632
GTATAGG2100.036.9412161
GTACATG40400.035.0069351
ACGCAGA26400.033.847847
TACATGG41200.033.7504582
ACTTGAC3900.033.6048288
CGCAGAG26800.033.3426488
ACATGGG41500.031.4589083
GAACAAA9200.031.1344031
CGGTTAC2150.030.42911128-29
GCAGAGT29350.028.8107819
CGCAAAA1453.9693477E-628.8105132
CATGGGG17200.028.0739924
CGCGGAA1700.027.9926282-83
Produced by FastQC (version 0.11.5)