##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940051_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 644196 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.03379251035399 34.0 31.0 34.0 26.0 34.0 2 31.169263702351458 34.0 31.0 34.0 26.0 34.0 3 31.283584499127596 34.0 31.0 34.0 27.0 34.0 4 34.19762153133519 37.0 35.0 37.0 30.0 37.0 5 34.55925525771659 37.0 37.0 37.0 31.0 37.0 6 34.36329471154742 37.0 37.0 37.0 31.0 37.0 7 34.379150134431136 37.0 37.0 37.0 31.0 37.0 8 34.27356736148626 37.0 37.0 37.0 31.0 37.0 9 35.74904687393278 39.0 39.0 39.0 30.0 39.0 10-11 35.864975876907025 39.0 39.0 39.0 30.5 39.0 12-13 35.79999875814193 39.0 38.5 39.0 30.0 39.0 14-15 37.27492409142559 41.0 39.0 41.0 30.0 41.0 16-17 37.365859924619215 41.0 39.0 41.0 30.5 41.0 18-19 37.39373110047253 41.0 39.0 41.0 30.0 41.0 20-21 37.42284491055517 41.0 39.0 41.0 30.0 41.0 22-23 37.497451086315344 41.0 39.0 41.0 30.0 41.0 24-25 37.61426025619532 41.0 39.0 41.0 30.0 41.0 26-27 37.67965262125192 41.0 39.0 41.0 30.0 41.0 28-29 37.63884671745866 41.0 39.0 41.0 30.0 41.0 30-31 37.57677477041149 41.0 39.0 41.0 30.0 41.0 32-33 37.5307701382809 41.0 39.0 41.0 30.0 41.0 34-35 37.442365367062195 41.0 38.5 41.0 30.0 41.0 36-37 37.30312358350564 41.0 38.0 41.0 30.0 41.0 38-39 37.19719541878558 41.0 38.0 41.0 30.0 41.0 40-41 37.09165223006663 41.0 38.0 41.0 30.0 41.0 42-43 36.93108463883662 40.5 38.0 41.0 29.0 41.0 44-45 36.793482884091176 40.0 37.5 41.0 29.0 41.0 46-47 36.63256679644084 40.0 37.0 41.0 29.0 41.0 48-49 36.48326052940409 40.0 37.0 41.0 29.0 41.0 50-51 36.31555303044415 40.0 36.0 41.0 29.0 41.0 52-53 36.40713540599445 40.0 36.0 41.0 29.0 41.0 54-55 36.188962675955764 40.0 35.5 41.0 29.0 41.0 56-57 36.06194620891778 40.0 35.0 41.0 29.0 41.0 58-59 35.845859024272116 39.5 35.0 41.0 29.0 41.0 60-61 35.58286297959006 39.0 35.0 41.0 28.5 41.0 62-63 35.2808213649262 39.0 35.0 41.0 28.0 41.0 64-65 34.96237170053834 38.0 35.0 41.0 28.0 41.0 66-67 34.634502542704396 37.0 35.0 40.0 28.0 41.0 68-69 34.29005768430726 37.0 35.0 40.0 27.5 41.0 70-71 33.91991567783718 36.0 34.5 39.0 27.0 41.0 72-73 33.55505079199499 36.0 34.0 39.0 26.0 41.0 74-75 33.19730020056008 35.0 34.0 37.5 26.0 40.0 76-77 32.84721885885662 35.0 34.0 37.0 26.0 39.0 78-79 32.51655552036958 35.0 34.0 37.0 25.5 39.0 80-81 32.21038627995206 35.0 34.0 36.0 25.0 38.0 82-83 31.918537215381654 35.0 33.5 36.0 24.5 37.0 84-85 31.68108541499792 35.0 33.0 35.5 24.0 37.0 86-87 31.476855646418173 35.0 33.0 35.0 24.0 36.5 88-89 31.316239157026743 35.0 33.0 35.0 23.0 36.0 90-91 31.17035808977392 35.0 33.0 35.0 22.5 36.0 92-93 31.02238061086998 35.0 33.0 35.0 20.0 36.0 94-95 30.91048143732653 35.0 33.0 35.0 20.0 35.5 96-97 30.803964166185445 35.0 33.0 35.0 19.0 35.0 98-99 30.732537147079462 35.0 33.0 35.0 18.5 35.0 100-101 30.631161944501365 35.0 33.0 35.0 17.5 35.0 102-103 29.962874653055902 34.0 31.0 35.0 17.5 35.0 104-105 30.809659637750002 35.0 33.0 35.0 19.0 35.0 106-107 30.903108681208824 35.0 33.0 35.0 19.0 35.0 108-109 30.873836534222505 35.0 33.0 35.0 18.0 35.0 110-111 30.812584213500237 35.0 33.0 35.0 17.0 35.0 112-113 30.750389632968847 35.0 33.0 35.0 14.0 35.0 114-115 30.66747076976572 35.0 33.0 35.0 7.5 35.0 116-117 30.581146110810995 35.0 33.0 35.0 2.0 35.0 118-119 30.477102465709194 35.0 33.0 35.0 2.0 35.0 120-121 30.370084570534434 35.0 33.0 35.0 2.0 35.0 122-123 30.222273966308393 35.0 33.0 35.0 2.0 35.0 124-125 29.007441834472736 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40205593090680125 1101 2 0.5155997567157478 1101 3 0.4687359681211589 1101 4 0.6142210290072683 1101 5 0.5632432487708812 1101 6 0.4498917799320594 1101 7 0.661281759636573 1101 8 0.47053088205858273 1101 9 0.645387830712103 1101 10-11 0.6185005565615924 1101 12-13 0.8099389581750174 1101 14-15 0.7656118905717619 1101 16-17 0.6922601604745466 1101 18-19 0.7573947309025826 1101 20-21 0.5753682653458867 1101 22-23 0.6043766795326917 1101 24-25 0.3862805729364638 1101 26-27 0.4787063765299351 1101 28-29 0.41418532727955437 1101 30-31 0.5810304523671803 1101 32-33 0.6207565213224839 1101 34-35 0.605000606437315 1101 36-37 0.6583871738537823 1101 38-39 0.6129369704211385 1101 40-41 0.6455046931581663 1101 42-43 0.604041360582741 1101 44-45 0.6570756190956217 1101 46-47 0.7220303784163917 1101 48-49 0.8129864774393383 1101 50-51 0.740858302206199 1101 52-53 0.7067579753355488 1101 54-55 0.749134166300145 1101 56-57 0.7519844365700195 1101 58-59 0.7604164113921783 1101 60-61 0.7079729748803842 1101 62-63 0.6287375288526533 1101 64-65 0.5912034713017107 1101 66-67 0.5248682476750872 1101 68-69 0.47484725369969283 1101 70-71 0.6190688048714605 1101 72-73 0.5728259041816202 1101 74-75 0.4179863756205293 1101 76-77 0.3941042439816087 1101 78-79 0.4189302477243473 1101 80-81 0.4057897317971637 1101 82-83 0.5449678219429153 1101 84-85 0.43592385074883566 1101 86-87 0.3117872670550561 1101 88-89 0.50901806010042 1101 90-91 0.5314166713000255 1101 92-93 0.5208489582834126 1101 94-95 0.6669044920359894 1101 96-97 0.6729230733745801 1101 98-99 0.46509212236723485 1101 100-101 0.6969294472109908 1101 102-103 0.7567370305353052 1101 104-105 0.7270036191344218 1101 106-107 0.7437757095816089 1101 108-109 0.7736016420205694 1101 110-111 0.8026217426661333 1101 112-113 0.8149136374216077 1101 114-115 0.6773684165053595 1101 116-117 0.6357107346416306 1101 118-119 0.5599328959400047 1101 120-121 0.5559449943888239 1101 122-123 0.5460545058632782 1101 124-125 0.6580778362488502 1102 1 0.26383026732209913 1102 2 0.1493816172410405 1102 3 0.29448381754606956 1102 4 0.22993076025587555 1102 5 0.17250374288064307 1102 6 0.2228895804237112 1102 7 0.3626694735744209 1102 8 0.22452765080499404 1102 9 0.5186125488067859 1102 10-11 0.43679893993954266 1102 12-13 0.27650676307895594 1102 14-15 0.3571809395415073 1102 16-17 0.44400848762072087 1102 18-19 0.4288029470449217 1102 20-21 0.30237461174024816 1102 22-23 0.4263002706696355 1102 24-25 0.5212998642502313 1102 26-27 0.4451539217392906 1102 28-29 0.48121310478087764 1102 30-31 0.4362178750277508 1102 32-33 0.3657454242554934 1102 34-35 0.4076494713516752 1102 36-37 0.48413999954191667 1102 38-39 0.5773633233841693 1102 40-41 0.5310863022162948 1102 42-43 0.46791650945595364 1102 44-45 0.5442911012457685 1102 46-47 0.4990456820845566 1102 48-49 0.6079121818293984 1102 50-51 0.585208252941193 1102 52-53 0.2764837168816996 1102 54-55 0.5319866267305571 1102 56-57 0.36876157628027073 1102 58-59 0.4342256745403006 1102 60-61 0.4639784952150592 1102 62-63 0.4297898426491784 1102 64-65 0.41916575786149224 1102 66-67 0.4299203159759628 1102 68-69 0.3679994178765611 1102 70-71 0.3677509244941959 1102 72-73 0.4622583115982408 1102 74-75 0.4370106299274923 1102 76-77 0.4819858819020695 1102 78-79 0.5921473355487521 1102 80-81 0.5230678153386705 1102 82-83 0.6061683952084387 1102 84-85 0.6221405968698406 1102 86-87 0.49236673631999395 1102 88-89 0.5383357148870864 1102 90-91 0.5243512068985545 1102 92-93 0.6176745999725526 1102 94-95 0.3923607324359466 1102 96-97 0.42132569287953814 1102 98-99 0.36753828749291984 1102 100-101 0.40470087748857253 1102 102-103 0.4717505825588191 1102 104-105 0.5374751698375668 1102 106-107 0.5888480478613474 1102 108-109 0.531013786093343 1102 110-111 0.5482882467748666 1102 112-113 0.5082638568748159 1102 114-115 0.3995502757036533 1102 116-117 0.4455710343453738 1102 118-119 0.4882780230171839 1102 120-121 0.4950338737675253 1102 122-123 0.5977512457473644 1102 124-125 0.3829584888691855 1103 1 -0.021713625807670667 1103 2 0.14428619739371484 1103 3 0.11000226538321911 1103 4 -0.04437903618178041 1103 5 -0.05603315279365262 1103 6 0.0017350002710401213 1103 7 0.13507087306551568 1103 8 0.27218668388387357 1103 9 0.3828682739976301 1103 10-11 0.23172578472325966 1103 12-13 0.3598623610433336 1103 14-15 0.36950123216237074 1103 16-17 0.3609343782059611 1103 18-19 0.2412425144749406 1103 20-21 0.12070317408121412 1103 22-23 0.18309734446098958 1103 24-25 0.1995457293901879 1103 26-27 0.36921403624310045 1103 28-29 0.3991568070709519 1103 30-31 0.3678956740099437 1103 32-33 0.32845534791961484 1103 34-35 0.363527333947097 1103 36-37 0.2680172260050284 1103 38-39 0.33407451931292087 1103 40-41 0.36074915208907044 1103 42-43 0.4259619929165055 1103 44-45 0.29987793330683843 1103 46-47 0.38329504595224506 1103 48-49 0.3803406169439043 1103 50-51 0.34390100103279764 1103 52-53 0.263570230876617 1103 54-55 0.42920831884251953 1103 56-57 0.3417847951097883 1103 58-59 0.33040092899322104 1103 60-61 0.34371450030411665 1103 62-63 0.2736845627509581 1103 64-65 0.22934069424825765 1103 66-67 0.3394893363321927 1103 68-69 0.36998415070099355 1103 70-71 0.430734385053988 1103 72-73 0.31168102152191324 1103 74-75 0.40887131695038903 1103 76-77 0.43045121269087616 1103 78-79 0.39487793351312916 1103 80-81 0.34108308251423836 1103 82-83 0.3109823265061529 1103 84-85 0.23724555870190045 1103 86-87 0.2969103113836056 1103 88-89 0.28532998969624757 1103 90-91 0.26732035448124947 1103 92-93 0.3473597144763687 1103 94-95 0.3175547527921836 1103 96-97 0.42253275394824286 1103 98-99 0.2863519007244726 1103 100-101 0.3096054576412399 1103 102-103 0.2779226563501709 1103 104-105 0.40210303243298995 1103 106-107 0.4988734931539689 1103 108-109 0.5251645494521178 1103 110-111 0.5108518600064222 1103 112-113 0.4881779790122174 1103 114-115 0.45716796018202643 1103 116-117 0.498878922386087 1103 118-119 0.3797299950273576 1103 120-121 0.5280888992128148 1103 122-123 0.35848279791021653 1103 124-125 0.4371537814900499 1104 1 -0.08482637090570933 1104 2 0.15198227582508395 1104 3 0.10527187322635712 1104 4 -2.3687931903992876E-4 1104 5 -0.11206256455835728 1104 6 -0.006426764434841914 1104 7 0.09460518679100716 1104 8 0.1597357034917195 1104 9 0.17076043086036918 1104 10-11 0.11652970629189241 1104 12-13 0.1491025571217648 1104 14-15 0.2680428988290444 1104 16-17 0.21643682918635676 1104 18-19 0.27452682820043606 1104 20-21 0.02689189957141025 1104 22-23 0.0021169523041209004 1104 24-25 0.1852442588019514 1104 26-27 0.19528021271369056 1104 28-29 0.29228180707094964 1104 30-31 0.270187340676614 1104 32-33 0.3282837792921711 1104 34-35 0.2851940006137639 1104 36-37 0.3585319318873772 1104 38-39 0.30049608794037397 1104 40-41 0.19476875993220233 1104 42-43 0.3391605223282781 1104 44-45 0.40292940389506526 1104 46-47 0.3800842616385154 1104 48-49 0.339642087532134 1104 50-51 0.197307863777894 1104 52-53 0.23097219166093197 1104 54-55 0.42172057374448 1104 56-57 0.18236077550194096 1104 58-59 0.17163867409126254 1104 60-61 0.10969489246097908 1104 62-63 0.24749583726076452 1104 64-65 0.20279657660120165 1104 66-67 0.0869280618223911 1104 68-69 0.1109645428578574 1104 70-71 0.09399418897556444 1104 72-73 0.1006638646591611 1104 74-75 0.1920575914601983 1104 76-77 0.20110072249479316 1104 78-79 0.21413038449352229 1104 80-81 0.12446543545541289 1104 82-83 0.20486713042771854 1104 84-85 0.19256418615287885 1104 86-87 0.119471585893411 1104 88-89 0.08839371518644512 1104 90-91 0.11716104075576084 1104 92-93 0.1312812831038208 1104 94-95 -0.001489364854876385 1104 96-97 0.10558422453647864 1104 98-99 0.10559209680290493 1104 100-101 0.164654477249087 1104 102-103 0.12232216615409186 1104 104-105 0.19872067949181016 1104 106-107 0.1941185911931811 1104 108-109 0.1930076867070234 1104 110-111 0.29675872275151605 1104 112-113 0.2383005280318251 1104 114-115 0.07530521508398635 1104 116-117 -0.029797548202147084 1104 118-119 -0.11984108340401534 1104 120-121 -0.17650668902248157 1104 122-123 -0.057399555030961835 1104 124-125 -0.05176778713740049 1105 1 0.4932650846546167 1105 2 0.5921389954472396 1105 3 0.5413495889841897 1105 4 0.5309281414337903 1105 5 0.4610106428267855 1105 6 0.496677214382963 1105 7 0.6479632088319391 1105 8 0.40792877634129354 1105 9 0.6830690039246363 1105 10-11 0.6946971959154737 1105 12-13 0.70478632698007 1105 14-15 0.7056189328922997 1105 16-17 0.6994365071597315 1105 18-19 0.6267747529177683 1105 20-21 0.5010939891663782 1105 22-23 0.5368108481106191 1105 24-25 0.487278393623761 1105 26-27 0.42437979050101404 1105 28-29 0.5720871598689996 1105 30-31 0.549992693474664 1105 32-33 0.5928957372140147 1105 34-35 0.6352548278643653 1105 36-37 0.6786711189490617 1105 38-39 0.6255282906024604 1105 40-41 0.5814243345708192 1105 42-43 0.6572378444980203 1105 44-45 0.551929332333593 1105 46-47 0.48239480264324186 1105 48-49 0.5649802154840486 1105 50-51 0.4953882273101726 1105 52-53 0.4880836128717476 1105 54-55 0.5361563115432517 1105 56-57 0.5574189191973815 1105 58-59 0.37373274164529136 1105 60-61 0.2959559844890265 1105 62-63 0.35776759194803986 1105 64-65 0.35886320033098684 1105 66-67 0.39104195190397206 1105 68-69 0.26278220422896936 1105 70-71 0.2231492269031392 1105 72-73 0.5079112526654583 1105 74-75 0.5325604896997831 1105 76-77 0.4771893513769996 1105 78-79 0.5313758045593602 1105 80-81 0.44276191463102776 1105 82-83 0.4863944311290247 1105 84-85 0.5112631986045741 1105 86-87 0.4078911507996672 1105 88-89 0.37935371232398296 1105 90-91 0.5209198070359946 1105 92-93 0.5743943860092138 1105 94-95 0.6746502515756347 1105 96-97 0.5273183378898487 1105 98-99 0.6159595649580503 1105 100-101 0.6424704211448926 1105 102-103 0.5050105517272954 1105 104-105 0.5692167793916241 1105 106-107 0.5857834688230668 1105 108-109 0.7108488560214639 1105 110-111 0.49500636122296626 1105 112-113 0.43740243156696224 1105 114-115 0.473880247286651 1105 116-117 0.5167621340649227 1105 118-119 0.6277302991636127 1105 120-121 0.5284006388721849 1105 122-123 0.3660922869613117 1105 124-125 0.3682577960885922 1106 1 -0.15564740643777597 1106 2 -0.11636432769380178 1106 3 -0.19155986997267505 1106 4 -0.1384470058433891 1106 5 -0.05377527648093405 1106 6 -0.031784544653113755 1106 7 -0.04244808842806691 1106 8 -0.19662311774973062 1106 9 0.07251529850171323 1106 10-11 -0.024290843048035526 1106 12-13 0.060353901300040036 1106 14-15 0.17404980275747306 1106 16-17 -0.011284845831376344 1106 18-19 -0.09819593359973311 1106 20-21 -0.07976721681726673 1106 22-23 0.10737826628128744 1106 24-25 0.11322061270406891 1106 26-27 0.13715379703656794 1106 28-29 0.04840782807445265 1106 30-31 0.1008952364370117 1106 32-33 0.09956970031168311 1106 34-35 0.13763468517229427 1106 36-37 0.02166512565496248 1106 38-39 -0.05039164171391519 1106 40-41 -0.043063566697398414 1106 42-43 -0.049921175111492744 1106 44-45 0.08507557041301794 1106 46-47 0.06358676123812756 1106 48-49 -0.005195409893907765 1106 50-51 -0.05271072592169901 1106 52-53 -0.059446105179389974 1106 54-55 0.07121750787402448 1106 56-57 -0.051748606290452415 1106 58-59 -0.057168353387176296 1106 60-61 -0.1312869582723124 1106 62-63 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108-109 -1.0077431324885815 2216 110-111 -0.9558260375677037 2216 112-113 -0.9895679778610962 2216 114-115 -0.9835496948044984 2216 116-117 -0.9311076005249639 2216 118-119 -0.9226874578028301 2216 120-121 -1.0099754538869163 2216 122-123 -1.0012813530331819 2216 124-125 -0.9834425796959323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14267.0 3 3167.0 4 2810.0 5 1884.0 6 1882.0 7 1839.0 8 1674.0 9 1713.0 10 1940.0 11 2419.0 12 2557.0 13 2436.0 14 2405.0 15 2355.0 16 2208.0 17 2289.0 18 1806.0 19 1505.0 20 1385.0 21 1182.0 22 1355.0 23 2406.0 24 5158.0 25 6867.0 26 8572.0 27 5344.0 28 4867.0 29 5661.0 30 6269.0 31 7401.0 32 9964.0 33 13766.0 34 20913.0 35 38024.0 36 96375.0 37 234367.0 38 122710.0 39 454.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99826047790263 19.6131116478089 13.284978760745819 23.103649113542644 2 15.086880831143615 21.66272284166982 39.06191416307678 24.188482164109793 3 18.721012383059662 27.275183416792824 27.18186405807228 26.821940142075228 4 12.34288911511233 18.258044963204153 34.82993091550555 34.56913500617797 5 13.510324117349299 40.71108930384798 31.363330051892312 14.415256526910406 6 31.20149832044614 37.54668662770744 16.75857752736749 14.493237524478936 7 28.50605021554669 35.47327096766178 18.74883530244866 17.271843514342862 8 25.655385896810323 37.80990001771523 18.303564491215422 18.231149594259023 9 25.880549873474383 20.22326952131661 18.2484440769226 35.6477365282864 10-11 24.464826104884533 30.048557222275285 24.879499968550018 20.607116704290167 12-13 24.727335689034962 27.231103336415778 25.62745595870612 22.41410501584314 14-15 22.211552867257613 28.207902861086293 23.321226159878343 26.259318111777752 16-17 22.53867344118314 31.365317664158315 23.9588994723455 22.137109422313046 18-19 22.573242293677446 29.98282133881725 25.58082824134621 21.863108126159094 20-21 23.68672791018171 28.857921474324076 25.347411253418116 22.1079393620761 22-23 23.38618746964546 28.751510441962118 25.362758620689657 22.499543467702768 24-25 23.127778968856905 28.834161392257574 25.454111864080264 22.583947774805257 26-27 22.654274123307513 29.237366421517258 25.383676682238853 22.724682772936376 28-29 22.599126602302878 29.09442040763734 25.64095996125132 22.665493028808463 30-31 22.681379858524213 28.888818165658474 25.594107478438254 22.83569449737906 32-33 22.554300999315366 28.996291184694034 25.404530995542903 23.044876820447698 34-35 23.02138001752753 28.950312010240175 25.260370779478354 22.767937192753944 36-37 23.243472327979863 28.485868838792754 25.25678731187753 23.01387152134985 38-39 22.640097557719194 28.621484898658117 25.6442977870202 23.094119756602495 40-41 22.71580942652377 28.51981019963502 25.915527504830006 22.848852869011203 42-43 22.88947315716791 28.469338723712777 25.506027304321233 23.13516081479808 44-45 22.685478080304293 28.668594383745077 25.653515496128392 22.992412039822234 46-47 22.861938250548658 28.99721381432623 25.30918587328561 22.831662061839502 48-49 22.80177402939585 28.82844347008596 25.404827577105298 22.964954923412886 50-51 22.926942489547578 28.80793468186285 25.349436197910602 22.91568663067897 52-53 23.040568864599667 28.557033954882083 25.47206135788477 22.93033582263348 54-55 22.8189383471254 28.54143054542067 25.499852505084693 23.139778602369233 56-57 22.853820291097154 29.013764583774854 25.29637211497631 22.836043010151684 58-59 22.954436592328182 28.455955476094175 25.400565321317863 23.189042610259783 60-61 22.652671355747877 28.727854493064577 25.709630418531937 22.909843732655613 62-63 22.333513701092066 28.94685154750103 25.78655998832397 22.93307476308294 64-65 22.438005203533308 28.62812904948947 25.575109998594687 23.358755748382535 66-67 22.49038046465694 29.05565510666116 25.196117403362102 23.257847025319798 68-69 22.455466885314127 28.739252033126334 25.683933293889826 23.121347787669713 70-71 22.357855182506686 28.757060173054306 25.750154304813144 23.134930339625864 72-73 22.338848159085334 28.774441763626367 25.497840337099937 23.388869740188362 74-75 22.33578863450944 29.168869296159933 25.3249218115046 23.17042025782603 76-77 22.63661606223127 28.878295517793063 25.393428332118482 23.091660087857186 78-79 22.49275470332907 28.899902723206765 25.516331709983177 23.09101086348099 80-81 22.469393617103865 29.308454891975188 25.38462732798708 22.837524162933864 82-83 22.644335015142495 28.994866069868962 25.37844587791137 22.98235303707718 84-85 22.588380691985165 28.88384806416028 25.425241955705435 23.10252928814912 86-87 22.52602256026124 28.87632694288973 25.694691917886175 22.902958578962856 88-89 22.478581050392275 29.018047430308346 25.44450871315494 23.058862806144433 90-91 22.41416121581491 29.143766619436732 25.570699326488228 22.871372838260125 92-93 22.50992675090494 28.715136024050924 25.580037486392936 23.194899738651202 94-95 22.480776636315078 28.724643172134506 25.677941888920635 23.11663830262978 96-97 22.503293848558275 28.807685051401116 25.574084612894183 23.11493648714642 98-99 22.347107848694108 28.798603034006405 25.483371042304963 23.370918074994528 100-101 22.067645211601867 28.89893121900966 25.808728028736834 23.22469554065164 102-103 22.30114728171795 28.453937535762787 25.863799795186495 23.381115387332773 104-105 21.945048290425763 29.015946709729512 25.806341417968387 23.232663581876338 106-107 22.104809562445894 29.098765096606638 25.48512952704093 23.31129581390654 108-109 22.33996798350421 29.079269551877747 25.250487933259112 23.33027453135893 110-111 21.90095861527139 29.559710831700446 25.498715946178425 23.040614606849736 112-113 21.786426068680985 29.51662754956666 25.680408505193657 23.016537876558697 114-115 21.654009382149077 29.63830674177975 25.475259491798962 23.232424384272207 116-117 21.676012954389495 29.644421880870542 25.58406535570232 23.09549980903764 118-119 21.62066266874948 29.745947993950328 25.198213670143883 23.43517566715631 120-121 21.49014797447324 29.919568964178687 25.48476002670683 23.105523034641244 122-123 21.50985141366622 29.729454872917536 25.545825764280277 23.214867949135964 124-125 21.68576396975484 29.84000186315152 25.337463319204435 23.136770847889203 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3641.0 1 3229.5 2 2763.5 3 2286.5 4 1448.5 5 874.0 6 655.5 7 600.0 8 633.5 9 610.0 10 512.0 11 435.0 12 394.0 13 382.5 14 391.5 15 386.0 16 394.0 17 462.5 18 539.0 19 581.0 20 660.0 21 839.5 22 1029.5 23 1274.0 24 1645.5 25 2121.0 26 2600.0 27 3105.5 28 3775.5 29 4728.5 30 5850.5 31 7136.0 32 9021.5 33 10349.5 34 10992.0 35 11838.0 36 13457.0 37 15019.5 38 15653.5 39 15979.0 40 16174.5 41 17398.0 42 18379.5 43 19054.5 44 20768.5 45 22384.5 46 22755.0 47 23050.0 48 23593.5 49 23424.5 50 23659.0 51 23778.0 52 23506.5 53 23941.0 54 24185.5 55 23167.5 56 20817.0 57 18877.0 58 17810.0 59 16140.0 60 13968.0 61 11618.5 62 9370.0 63 7239.0 64 5651.0 65 4449.0 66 3273.0 67 2596.0 68 1873.0 69 1172.0 70 865.0 71 638.5 72 543.0 73 440.5 74 328.0 75 272.0 76 194.0 77 115.0 78 68.0 79 39.5 80 25.5 81 19.5 82 13.5 83 11.0 84 9.5 85 10.5 86 6.5 87 4.0 88 7.0 89 4.5 90 5.0 91 6.0 92 3.5 93 1.5 94 1.5 95 3.0 96 3.5 97 2.0 98 1.0 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05293420015026483 2 0.22027457481884394 3 0.026544716204385 4 0.12123639389254202 5 0.026389483945879824 6 0.04268887108892325 7 0.039118529143304215 8 0.10586840030052966 9 0.13163695521238877 10-11 0.049131009816888026 12-13 0.03516010655142224 14-15 0.013582822619202852 16-17 0.03321970332010755 18-19 0.05805686468093561 20-21 0.20149147153971772 22-23 0.11774366807617558 24-25 0.08103123893970159 26-27 0.01536799359201237 28-29 0.006985451632732896 30-31 0.008925854864047589 32-33 0.00853777421778465 34-35 0.010322945190594167 36-37 0.005355512918428553 38-39 0.010012480673583816 40-41 0.0070630677619854835 42-43 0.0132723581021925 44-45 0.012961893585182148 46-47 0.01948164844239952 48-49 0.01948164844239952 50-51 0.013505206489950263 52-53 0.016454619401548597 54-55 0.016454619401548597 56-57 0.017929325857347765 58-59 0.021344435544461623 60-61 0.011720035517140747 62-63 0.021965364578482326 64-65 0.03267639041533943 66-67 0.030115058150004037 68-69 0.03679004526572658 70-71 0.027708958143173815 72-73 0.01769647746959 74-75 0.012340964551161447 76-77 0.012806661326676976 78-79 0.02444908071456513 80-81 0.020335425864177983 82-83 0.02289675812951338 84-85 0.018860719408378815 86-87 0.029106048469720397 88-89 0.030813603313277326 90-91 0.028485119435699695 92-93 0.035548187197685176 94-95 0.029183664598972984 96-97 0.02988220976224627 98-99 0.035548187197685176 100-101 0.02243106135399785 102-103 0.030347906537761798 104-105 0.027320877496910877 106-107 0.03391824848338083 108-109 0.023750535551291842 110-111 0.022042980707734913 112-113 0.029416512986730748 114-115 0.01560084197977013 116-117 0.013970903265465792 118-119 0.030115058150004037 120-121 0.025768554911859122 122-123 0.019559264571652107 124-125 0.019559264571652107 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 644196.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.03982477823752 #Duplication Level Percentage of deduplicated Percentage of total 1 73.65885420781282 38.33193866340323 2 13.358471782917169 13.903450617760793 3 5.165141600324918 8.063791915070821 4 2.5253561674000666 5.256763698165637 5 1.4281544365831602 3.7160453318025097 6 0.8850526519854157 2.763479095730128 7 0.5969003021089777 2.174381099227977 8 0.40072624830498327 1.6682978996665463 9 0.3026573984086731 1.4175214182922118 >10 1.5375513795846638 14.425653243156356 >50 0.10136520428943162 3.5929311297185706 >100 0.03857257154959132 3.7220815475409714 >500 8.970365476636891E-4 0.30296374469300674 >1k 2.99012182554563E-4 0.6607005957711899 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4246 0.6591161696129749 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 802 0.12449627132115071 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.761612925258773E-5 0.0 22-23 0.0 0.0 0.0 3.8808064626293863E-4 0.0 24-25 0.0 0.0 0.0 0.0012418580680414034 0.0 26-27 0.0 0.0 0.0 0.0018627871020621052 0.0 28-29 0.0 0.0 0.0 0.003182261299356097 0.0 30-31 0.0 0.0 0.0 0.007916845183763947 0.0 32-33 0.0 0.0 0.0 0.01567845810902272 0.0 34-35 0.0 0.0 0.0 0.02514762587783842 0.0 36-37 0.0 0.0 0.0 0.0423784065719129 0.0 38-39 0.0 0.0 0.0 0.06861265825928754 0.0 40-41 0.0 0.0 0.0 0.10322945190594167 0.0 42-43 0.0 0.0 0.0 0.1366043874845544 0.0 44-45 0.0 0.0 0.0 0.16834938434886276 0.0 46-47 0.0 0.0 0.0 0.21189203285956448 0.0 48-49 0.0 0.0 0.0 0.26226490074449393 0.0 50-51 0.0 0.0 0.0 0.32296071382001756 0.0 52-53 0.0 0.0 0.0 0.3767486913920608 0.0 54-55 0.0 0.0 0.0 0.43410701090972315 0.0 56-57 0.0 0.0 0.0 0.5007016498084434 0.0 58-59 0.0 0.0 0.0 0.5772311532514949 0.0 60-61 0.0 0.0 0.0 0.6598147147762483 0.0 62-63 0.0 0.0 0.0 0.7444162956615687 0.0 64-65 0.0 0.0 0.0 0.8308806636489516 0.0 66-67 0.0 0.0 0.0 0.9316263994188104 0.0 68-69 0.0 0.0 0.0 1.0614781836583895 0.0 70-71 0.0 0.0 0.0 1.2013424485715527 0.0 72-73 0.0 0.0 0.0 1.334764574756751 0.0 74-75 0.0 0.0 0.0 1.472921284826357 0.0 76-77 0.0 0.0 0.0 1.6213233239573048 0.0 78-79 0.0 0.0 0.0 1.8042645406056543 0.0 80-81 0.0 0.0 0.0 2.0176312799210177 0.0 82-83 0.0 0.0 0.0 2.239768641841924 0.0 84-85 0.0 0.0 0.0 2.447624635980354 0.0 86-87 0.0 0.0 0.0 2.6623108494930117 0.0 88-89 0.0 0.0 0.0 2.9035417792100544 0.0 90-91 0.0 0.0 0.0 3.187228731628262 0.0 92-93 0.0 0.0 0.0 3.4816267098833276 0.0 94-95 0.0 0.0 0.0 3.7852610075194506 0.0 96-97 0.0 0.0 0.0 4.080202298679284 0.0 98-99 0.0 0.0 0.0 4.41270979639737 0.0 100-101 0.0 0.0 0.0 4.801411371694329 0.0 102-103 0.0 0.0 0.0 5.197641711528789 0.0 104-105 0.0 0.0 0.0 5.589758396512862 0.0 106-107 0.0 0.0 0.0 5.9897919266807 0.0 108-109 0.0 0.0 0.0 6.41000565045421 0.0 110-111 0.0 0.0 0.0 6.895805003446156 0.0 112-113 0.0 0.0 0.0 7.406286285540425 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1670 0.0 56.100967 1 ATCAACG 2040 0.0 44.710434 3 TCAACGC 2080 0.0 44.137226 4 CAACGCA 2100 0.0 43.686275 5 GGTATCA 975 0.0 43.455112 1 AACGCAG 2210 0.0 41.242287 6 TATCAAC 2265 0.0 41.103363 2 GTATAGG 210 0.0 36.941216 1 GTACATG 4040 0.0 35.006935 1 ACGCAGA 2640 0.0 33.84784 7 TACATGG 4120 0.0 33.750458 2 ACTTGAC 390 0.0 33.604828 8 CGCAGAG 2680 0.0 33.342648 8 ACATGGG 4150 0.0 31.458908 3 GAACAAA 920 0.0 31.134403 1 CGGTTAC 215 0.0 30.429111 28-29 GCAGAGT 2935 0.0 28.810781 9 CGCAAAA 145 3.9693477E-6 28.810513 2 CATGGGG 1720 0.0 28.073992 4 CGCGGAA 170 0.0 27.99262 82-83 >>END_MODULE