FastQCFastQC Report
Fri 27 May 2016
SRR940051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644196
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85251.3233550037566206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63450.9849486802153381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42570.6608237244565319No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.2331588522747735No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.2187222522337922No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.17758570372992072No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.1561636520562065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6620.10276375513042614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24300.076.5420151
GTATCAA47100.059.3613131
TATAACG655.419988E-755.007332
ATCAACG60450.045.7408643
TATCAAC60900.045.4028782
TCAACGC61100.045.2542574
CAACGCA61950.044.6774255
AACGCAG63550.043.632696
GTGGTAT9450.042.8971751
TAACGCA853.442021E-642.064434
TGGTATC9700.039.3179552
ACGCAGA71800.038.4474687
CGCAGAG71850.038.3379068
ATCACGC957.3890114E-637.6365933
GTGTACG650.001920064336.6858181
GCAGAGT76300.035.9469
TATCACG1102.0171246E-532.504332
ATAACGC1152.7333199E-531.0910993
GTATAAC1551.9356594E-730.768751
GAGTACT55600.030.656612-13