##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940051_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 644196 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.14517010350887 34.0 33.0 34.0 31.0 34.0 2 33.174420517979 34.0 33.0 34.0 31.0 34.0 3 33.23315729995219 34.0 33.0 34.0 31.0 34.0 4 36.46780017261827 37.0 37.0 37.0 35.0 37.0 5 36.48887605635552 37.0 37.0 37.0 35.0 37.0 6 36.57569901086005 37.0 37.0 37.0 35.0 37.0 7 36.56557165831517 37.0 37.0 37.0 35.0 37.0 8 36.562454594564386 37.0 37.0 37.0 35.0 37.0 9 38.436829784723905 39.0 39.0 39.0 37.0 39.0 10-11 38.42076945525896 39.0 39.0 39.0 37.0 39.0 12-13 38.39795108941999 39.0 39.0 39.0 37.0 39.0 14-15 39.95177088960503 41.0 40.0 41.0 38.0 41.0 16-17 39.967195542971396 41.0 40.0 41.0 38.0 41.0 18-19 40.04925286713981 41.0 40.0 41.0 38.0 41.0 20-21 40.05578504057772 41.0 40.0 41.0 38.0 41.0 22-23 39.99962511409571 41.0 40.0 41.0 38.0 41.0 24-25 39.96237946215127 41.0 40.0 41.0 38.0 41.0 26-27 39.85513027091133 41.0 40.0 41.0 37.0 41.0 28-29 39.75779033089309 41.0 40.0 41.0 37.0 41.0 30-31 39.523714055970544 41.0 39.5 41.0 36.0 41.0 32-33 39.25977807996324 41.0 39.0 41.0 35.0 41.0 34-35 39.114941725810155 41.0 39.0 41.0 35.0 41.0 36-37 38.98938521816342 41.0 39.0 41.0 35.0 41.0 38-39 38.84957761302461 41.0 39.0 41.0 35.0 41.0 40-41 38.688893597600725 41.0 38.5 41.0 35.0 41.0 42-43 38.48000298045936 41.0 38.0 41.0 35.0 41.0 44-45 38.17998637060771 41.0 38.0 41.0 35.0 41.0 46-47 37.70663742090916 41.0 38.0 41.0 33.5 41.0 48-49 37.28972393495147 40.5 37.5 41.0 33.0 41.0 50-51 37.23579003905644 41.0 37.0 41.0 33.0 41.0 52-53 37.11234236164149 41.0 37.0 41.0 33.0 41.0 54-55 36.94652326310626 40.5 36.5 41.0 33.0 41.0 56-57 36.752031214102544 40.0 36.0 41.0 32.5 41.0 58-59 36.52594474352526 40.0 35.0 41.0 32.0 41.0 60-61 36.2805931735062 39.0 35.0 41.0 32.0 41.0 62-63 36.00760638066676 39.0 35.0 41.0 31.0 41.0 64-65 35.69513936752169 38.5 35.0 41.0 31.0 41.0 66-67 35.36499372861675 37.0 35.0 41.0 31.0 41.0 68-69 35.01212984867959 37.0 35.0 40.0 30.5 41.0 70-71 34.64037342672106 36.5 35.0 39.0 30.0 41.0 72-73 34.27904473172761 36.0 35.0 39.0 30.0 41.0 74-75 33.93198809058113 35.5 35.0 38.0 29.5 40.5 76-77 33.589813503964635 35.0 35.0 37.0 29.0 39.0 78-79 33.2769685002701 35.0 35.0 37.0 29.0 39.0 80-81 32.98799200864333 35.0 35.0 36.0 29.0 38.5 82-83 32.73491297679588 35.0 35.0 36.0 28.5 37.0 84-85 32.51054880812672 35.0 35.0 36.0 27.5 37.0 86-87 32.32187796881695 35.0 34.0 35.0 27.0 36.5 88-89 32.233097535532664 35.0 34.5 35.0 27.5 36.0 90-91 32.235134182764256 35.0 35.0 35.0 29.0 36.0 92-93 32.15008320449056 35.0 35.0 35.0 29.0 36.0 94-95 32.07628578879719 35.0 35.0 35.0 27.5 36.0 96-97 32.0115376376134 35.0 35.0 35.0 27.0 35.5 98-99 31.952529199187826 35.0 35.0 35.0 27.0 35.0 100-101 31.89065594943154 35.0 35.0 35.0 27.0 35.0 102-103 31.418709523188596 35.0 33.5 35.0 25.0 35.0 104-105 31.779346658470402 35.0 34.0 35.0 25.5 35.0 106-107 31.81917847984154 35.0 35.0 35.0 27.0 35.0 108-109 31.787168035815185 35.0 35.0 35.0 25.5 35.0 110-111 31.738813963452117 35.0 35.0 35.0 25.0 35.0 112-113 31.699835143341467 35.0 35.0 35.0 25.0 35.0 114-115 31.63261724692485 35.0 35.0 35.0 25.0 35.0 116-117 31.57124694968612 35.0 34.0 35.0 24.5 35.0 118-119 31.491452135685414 35.0 34.0 35.0 24.0 35.0 120-121 31.4095702239691 35.0 34.0 35.0 21.5 35.0 122-123 31.30207576576073 35.0 34.0 35.0 18.5 35.0 124-125 30.290535023502166 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19048559389631237 1101 2 0.19813159436474592 1101 3 0.15112204968870913 1101 4 0.17639670736603108 1101 5 0.11418181845803588 1101 6 0.14591362045010925 1101 7 0.18505403769520257 1101 8 0.15713830109428528 1101 9 0.2086365056633781 1101 10-11 0.18270192851292677 1101 12-13 0.20791972850837226 1101 14-15 0.2863481208205485 1101 16-17 0.31731437040932775 1101 18-19 0.15157022758991445 1101 20-21 0.15840972570608614 1101 22-23 0.21417654383825635 1101 24-25 0.23887857924957956 1101 26-27 0.24928742521267822 1101 28-29 0.2931107970930711 1101 30-31 0.4164730898240805 1101 32-33 0.41077898477094266 1101 34-35 0.4440680506915129 1101 36-37 0.4498577433313429 1101 38-39 0.5221586317293472 1101 40-41 0.521673293449993 1101 42-43 0.5883683419076249 1101 44-45 0.7076848593757958 1101 46-47 0.8831576850096781 1101 48-49 1.028757943562482 1101 50-51 0.830009163557051 1101 52-53 0.8296097655945474 1101 54-55 0.7469985644666508 1101 56-57 0.7107709146747538 1101 58-59 0.7332600936785099 1101 60-61 0.7337606834839079 1101 62-63 0.6995747152028216 1101 64-65 0.8192495417594685 1101 66-67 0.7184051944068557 1101 68-69 0.7608352437681987 1101 70-71 0.7550097455973628 1101 72-73 0.7798552986945282 1101 74-75 0.664874688282211 1101 76-77 0.7222990113148597 1101 78-79 0.7262153990820721 1101 80-81 0.640583876187776 1101 82-83 0.7228434978359886 1101 84-85 0.6203506118182034 1101 86-87 0.6072644067132202 1101 88-89 0.5007242416102855 1101 90-91 0.5341823104655461 1101 92-93 0.5436875141856703 1101 94-95 0.691288515208015 1101 96-97 0.6922958188706474 1101 98-99 0.7073763363633248 1101 100-101 0.621201247535339 1101 102-103 0.6963702362106012 1101 104-105 0.6370593467580896 1101 106-107 0.6665659668944457 1101 108-109 0.5853497284069356 1101 110-111 0.5892518403455576 1101 112-113 0.5106169882691916 1101 114-115 0.6394351948062997 1101 116-117 0.6196266712771745 1101 118-119 0.5871909762715326 1101 120-121 0.6011966661352339 1101 122-123 0.7444492130831826 1101 124-125 0.7122619137997681 1102 1 0.11572387396703476 1102 2 0.08680097475362203 1102 3 0.12245711476164445 1102 4 -0.054153567821323634 1102 5 0.043357981493691966 1102 6 0.10263008292410802 1102 7 0.044479577868571596 1102 8 0.06811626951823513 1102 9 0.13136840782340897 1102 10-11 0.13778617664931403 1102 12-13 0.16588523305170355 1102 14-15 0.26463585499519837 1102 16-17 0.232173824148326 1102 18-19 0.2536242631735206 1102 20-21 0.2806151392169767 1102 22-23 0.24191921131438932 1102 24-25 0.2805158030255015 1102 26-27 0.31047970470616093 1102 28-29 0.33183773625990653 1102 30-31 0.34407426554921017 1102 32-33 0.2900548389465243 1102 34-35 0.45835407303162157 1102 36-37 0.41407094635292907 1102 38-39 0.3473151352077579 1102 40-41 0.3886536305752344 1102 42-43 0.4455670042880726 1102 44-45 0.4379216299800106 1102 46-47 0.4711363600630065 1102 48-49 0.5842042977861723 1102 50-51 0.623184882046516 1102 52-53 0.5537390024679993 1102 54-55 0.5743811163607759 1102 56-57 0.5755069570857856 1102 58-59 0.5884077062332977 1102 60-61 0.6743814225585254 1102 62-63 0.6842610945068728 1102 64-65 0.6023989192289321 1102 66-67 0.463798004038253 1102 68-69 0.4601992773281154 1102 70-71 0.5532712051021917 1102 72-73 0.5007225950243708 1102 74-75 0.5734383396651168 1102 76-77 0.49640154224232447 1102 78-79 0.5043014287206944 1102 80-81 0.5420770869059908 1102 82-83 0.43232593328556135 1102 84-85 0.4519795749971749 1102 86-87 0.44249037882624265 1102 88-89 0.47321960539171215 1102 90-91 0.5823044346000898 1102 92-93 0.641725705347163 1102 94-95 0.5830008805586289 1102 96-97 0.6216386867243173 1102 98-99 0.7072710054584093 1102 100-101 0.6164820912441797 1102 102-103 0.6952704820380085 1102 104-105 0.708863507577643 1102 106-107 0.6235312425296726 1102 108-109 0.6580836210722545 1102 110-111 0.6179706847908868 1102 112-113 0.6112212725000816 1102 114-115 0.7000908672487505 1102 116-117 0.7082862650150474 1102 118-119 0.59452847639594 1102 120-121 0.6827078577512289 1102 122-123 0.7989334897501514 1102 124-125 0.656431557930599 1103 1 0.11705339050647723 1103 2 0.14308596203098034 1103 3 0.10477263639014467 1103 4 -0.19761730827934088 1103 5 0.008850984038218712 1103 6 0.0892235943744879 1103 7 0.08984217328841737 1103 8 0.17055583694826026 1103 9 0.17849308975725364 1103 10-11 0.14832529878672318 1103 12-13 0.15428217961658675 1103 14-15 0.25909991351937833 1103 16-17 0.12008571982261884 1103 18-19 0.2835161206798773 1103 20-21 0.19125604252486283 1103 22-23 0.25898505100905567 1103 24-25 0.23397636282194156 1103 26-27 0.21282067162728424 1103 28-29 0.2959725963108042 1103 30-31 0.2865138329792316 1103 32-33 0.26983537329766705 1103 34-35 0.3920977117084732 1103 36-37 0.34378945780331094 1103 38-39 0.3193237229940209 1103 40-41 0.32757697663121377 1103 42-43 0.3778761645934168 1103 44-45 0.08540731700291104 1103 46-47 0.18447287405791712 1103 48-49 0.32640054460550516 1103 50-51 0.29506783370046463 1103 52-53 0.366383722569779 1103 54-55 0.400133342823878 1103 56-57 0.27024614283642023 1103 58-59 0.40134027620784707 1103 60-61 0.407630468360054 1103 62-63 0.35067966957048924 1103 64-65 0.375652736022829 1103 66-67 0.3603787928423259 1103 68-69 0.38482236893116806 1103 70-71 0.31345091248132206 1103 72-73 0.19668792581317263 1103 74-75 0.17193547706969525 1103 76-77 0.1221458170514893 1103 78-79 0.22124799360619107 1103 80-81 0.25580959326731545 1103 82-83 0.30271556432881397 1103 84-85 0.19826456227453093 1103 86-87 0.22848751623082109 1103 88-89 0.29181534330519554 1103 90-91 0.31156334045250134 1103 92-93 0.37170821170848 1103 94-95 0.4106789976069649 1103 96-97 0.4096507732383188 1103 98-99 0.2599268578757119 1103 100-101 0.26561536096428284 1103 102-103 0.2929804057021279 1103 104-105 0.2906748943460933 1103 106-107 0.25556368527775675 1103 108-109 0.3143018144564742 1103 110-111 0.32954193161022616 1103 112-113 0.24446395697844991 1103 114-115 0.3177435390044785 1103 116-117 0.3705906543280264 1103 118-119 0.3545475603654076 1103 120-121 0.4195017508810004 1103 122-123 0.5298908688850048 1103 124-125 0.49379956047512863 1104 1 0.15072986109471032 1104 2 0.16705655026627397 1104 3 0.18001773442935587 1104 4 -0.012935935730318704 1104 5 0.06382647423429688 1104 6 0.10282653555096033 1104 7 0.04611668309234318 1104 8 0.11910975851688477 1104 9 0.1095715211298014 1104 10-11 0.16194049486515638 1104 12-13 0.14719884628325985 1104 14-15 0.2305092272448661 1104 16-17 0.29308817080301 1104 18-19 0.22871219911124285 1104 20-21 0.21175849350525056 1104 22-23 0.22752671767571542 1104 24-25 0.16900087262586538 1104 26-27 0.20554125986257077 1104 28-29 0.303497106114726 1104 30-31 0.2541363819988405 1104 32-33 0.3696883144741321 1104 34-35 0.3030535940614101 1104 36-37 0.21512524211703976 1104 38-39 0.32186048769990094 1104 40-41 0.37212354525865976 1104 42-43 0.31044969400518596 1104 44-45 0.3400274150421225 1104 46-47 0.4973160113128188 1104 48-49 0.38030985833099606 1104 50-51 0.5621021474259535 1104 52-53 0.6343126441384044 1104 54-55 0.6283073624317268 1104 56-57 0.5445843781305371 1104 58-59 0.6696736095411779 1104 60-61 0.580804487967896 1104 62-63 0.6342826107469506 1104 64-65 0.5171478340620439 1104 66-67 0.5798640869599652 1104 68-69 0.4250061924605788 1104 70-71 0.3219435595401521 1104 72-73 0.2860139062053264 1104 74-75 0.23939871236380839 1104 76-77 0.37427816999266383 1104 78-79 0.2419342681159975 1104 80-81 0.28810125993398117 1104 82-83 0.32223762315234694 1104 84-85 0.41029887600002013 1104 86-87 0.46384290838768294 1104 88-89 0.2877589707561796 1104 90-91 0.27472510515838877 1104 92-93 0.338730270532011 1104 94-95 0.2812917427050081 1104 96-97 0.2869301850030226 1104 98-99 0.19289254415021873 1104 100-101 0.23528447861134083 1104 102-103 0.20131373903546645 1104 104-105 0.2925008747382485 1104 106-107 0.30759799900324936 1104 108-109 0.25229201053491224 1104 110-111 0.2749831080808143 1104 112-113 0.3372213099196273 1104 114-115 0.34151804880839975 1104 116-117 0.3081029092299872 1104 118-119 0.27489069762031093 1104 120-121 0.298521358724134 1104 122-123 0.3579300845712794 1104 124-125 0.2571696585143428 1105 1 0.12652419342618515 1105 2 0.13641929536431263 1105 3 0.11243686996678548 1105 4 0.005652156440859812 1105 5 0.045113149488344106 1105 6 0.08437262113847765 1105 7 0.08049302486993071 1105 8 0.0994214087244174 1105 9 0.10175343038985574 1105 10-11 0.09003910657261116 1105 12-13 0.10408753241463842 1105 14-15 0.21011420792326874 1105 16-17 0.17176911398320982 1105 18-19 0.23988473381856323 1105 20-21 0.20966084787766448 1105 22-23 0.17696406560758504 1105 24-25 0.20314911221367282 1105 26-27 0.2402172653012471 1105 28-29 0.1866416960674897 1105 30-31 0.17160811521767982 1105 32-33 0.268737441424193 1105 34-35 0.38916887959167923 1105 36-37 0.2960567935697327 1105 38-39 0.24606794440520474 1105 40-41 0.2988893245630919 1105 42-43 0.2805951426956028 1105 44-45 0.3631551880491415 1105 46-47 0.3798165480238538 1105 48-49 0.5531173937539222 1105 50-51 0.586187055111651 1105 52-53 0.5127386495770807 1105 54-55 0.22390007031779646 1105 56-57 0.16166341777559268 1105 58-59 0.14275755114702093 1105 60-61 0.24490388685153164 1105 62-63 0.29382443842693107 1105 64-65 0.22845991363840312 1105 66-67 0.23174436461847847 1105 68-69 0.3293463956951541 1105 70-71 0.2802590025056517 1105 72-73 0.20091389904918344 1105 74-75 0.2667575573616645 1105 76-77 0.24313730123640198 1105 78-79 0.29358376002954856 1105 80-81 0.29281537185612194 1105 82-83 0.2350041896329529 1105 84-85 0.22300908782197837 1105 86-87 0.2452986476176804 1105 88-89 0.277230489290595 1105 90-91 0.29716857159605325 1105 92-93 0.273242579105073 1105 94-95 0.28539310952911023 1105 96-97 0.26464773187578317 1105 98-99 0.2824785610387295 1105 100-101 0.3554496067063724 1105 102-103 0.31171519888947685 1105 104-105 0.29620865347018466 1105 106-107 0.15703266337994393 1105 108-109 0.19901896774115002 1105 110-111 0.3910200338000074 1105 112-113 0.4197361589249269 1105 114-115 0.4043311302453567 1105 116-117 0.34604837938885424 1105 118-119 0.3312811370077462 1105 120-121 0.3675066170610499 1105 122-123 0.37553867910398253 1105 124-125 0.3005224923486054 1106 1 0.149605899257935 1106 2 0.1449762770834866 1106 3 0.15267228633179997 1106 4 -0.03929612974958019 1106 5 0.007685581471122305 1106 6 0.043912917595022805 1106 7 0.04777974621724468 1106 8 0.13592485678489652 1106 9 0.11025505008397118 1106 10-11 0.17346896856501814 1106 12-13 0.18688427996664814 1106 14-15 0.2414285307889159 1106 16-17 0.211078281082834 1106 18-19 0.25565439372537924 1106 20-21 0.2586867601444638 1106 22-23 0.2938311711957482 1106 24-25 0.21350961836453308 1106 26-27 0.24359079998200173 1106 28-29 0.24481156947299354 1106 30-31 0.2184756182767842 1106 32-33 0.11390334295927573 1106 34-35 0.0958885518484891 1106 36-37 0.051678168421744886 1106 38-39 0.0926966809870109 1106 40-41 0.11917922283891613 1106 42-43 0.1716597118512908 1106 44-45 0.07533511746965615 1106 46-47 0.03894049366118679 1106 48-49 0.07993248742930348 1106 50-51 0.08654974735274124 1106 52-53 -0.06138684335787303 1106 54-55 -0.04824544947483389 1106 56-57 -0.058041488435456756 1106 58-59 0.02602814085195604 1106 60-61 -0.06311267633073214 1106 62-63 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60-61 -0.8320073348744472 2216 62-63 -0.7481545622354417 2216 64-65 -0.7638924122251467 2216 66-67 -0.7625827974541721 2216 68-69 -0.8211092348424245 2216 70-71 -0.7019584540954966 2216 72-73 -0.6041560768822478 2216 74-75 -0.6654044824990422 2216 76-77 -0.6426722422476985 2216 78-79 -0.7038733001943456 2216 80-81 -0.597062711314905 2216 82-83 -0.5565280340539331 2216 84-85 -0.5608459498548513 2216 86-87 -0.535570772178886 2216 88-89 -0.6494767725977653 2216 90-91 -0.5055289776067937 2216 92-93 -0.5304329607982581 2216 94-95 -0.6281821883768615 2216 96-97 -0.5488970704274543 2216 98-99 -0.5523541394288713 2216 100-101 -0.5432205554777667 2216 102-103 -0.6845802411981374 2216 104-105 -0.6603375719611826 2216 106-107 -0.6036580128354458 2216 108-109 -0.5974400992901501 2216 110-111 -0.6938610333493394 2216 112-113 -0.7267304500296348 2216 114-115 -0.7200933882192437 2216 116-117 -0.7867189817905711 2216 118-119 -0.6328176687451048 2216 120-121 -0.7218594351028287 2216 122-123 -0.7123968669640526 2216 124-125 -0.9424184314386217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 3.0 4 16.0 5 52.0 6 142.0 7 280.0 8 426.0 9 716.0 10 1237.0 11 1928.0 12 2427.0 13 4047.0 14 8044.0 15 8477.0 16 3449.0 17 2674.0 18 2575.0 19 2072.0 20 1767.0 21 1593.0 22 1629.0 23 1601.0 24 1983.0 25 2261.0 26 2824.0 27 3300.0 28 3816.0 29 4704.0 30 5625.0 31 6839.0 32 8756.0 33 11691.0 34 17269.0 35 39022.0 36 83934.0 37 250954.0 38 155056.0 39 995.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.950704498699686 18.039669293172377 14.13624189729457 21.873384310833366 2 16.11170100468189 20.024247730462104 40.49898786682066 23.365063398035343 3 19.055905519144854 25.932715920980325 29.382918810172093 25.62845974970273 4 12.34047580922236 16.781734995126243 36.604165468308146 34.27362372734325 5 12.943182559378311 37.83657026782747 32.993281795000016 16.226965377794205 6 31.012201313127708 36.94642693573002 16.435521206748525 15.605850544393746 7 27.821811995107083 34.446193394556936 19.746164210892335 17.985830399443646 8 27.017864128308776 34.00983551589889 18.804059634024426 20.168240721767912 9 27.871331085570226 15.79270905128253 19.459760693950287 36.87619916919695 10-11 25.4963551465703 26.91447944414433 25.527091133754322 22.06207427553105 12-13 26.95297704425361 24.46460394041565 27.019882147669342 21.562536867661393 14-15 23.573260312078933 24.795559115548684 26.748380927543792 24.882799644828594 16-17 21.503470993300176 28.48946593893784 27.04145943160156 22.965603636160424 18-19 21.313001012114324 25.176188613403372 32.08332557172041 21.427484802761892 20-21 22.4024986184329 24.054480313444977 32.645343963638396 20.897677104483726 22-23 22.00471595601339 24.054790777961987 32.71449993480245 21.225993331222174 24-25 21.750523132711162 24.34127191103329 32.667775024992395 21.240429931263154 26-27 21.353594247713428 24.7780178703376 32.584570534433624 21.283817347515352 28-29 21.31412072431484 24.51524771226104 32.956635801271354 21.213995762152766 30-31 21.40522558757396 24.2857952283119 32.86634259320346 21.44263659091068 32-33 21.32894542801481 24.476051506143325 32.59641878623709 21.598584279604776 34-35 21.720742075744383 24.50321565121012 32.38959442278944 21.386447850256058 36-37 21.938859463386464 24.06890535061193 32.20395288331977 21.788282302681825 38-39 21.497330686011367 24.29401729038628 32.34829773989286 21.8603542837095 40-41 21.6946395196493 24.247666859154666 32.30732572074337 21.75036790045266 42-43 21.915530738745115 24.229455111636838 31.78673273443891 22.068281415179133 44-45 21.80827365881752 24.528826167475696 31.581492388103495 22.081407785603293 46-47 22.232614167593535 24.926982569732324 30.73166453351506 22.108738729159082 48-49 22.400647640543976 25.142175882841016 30.105350252916672 22.351826223698335 50-51 22.548941989906563 25.438735386482993 29.584231760949177 22.42809086266127 52-53 22.666351027251068 25.43430172540923 29.433401376912272 22.465945870427433 54-55 22.270085501927987 25.578550627448788 29.36831337046489 22.783050500158335 56-57 22.39748270680427 25.814674068278627 29.28712914322616 22.500714081690944 58-59 22.590717974232376 25.18013196611819 29.46953532433844 22.759614735310986 60-61 22.406960101585565 25.37822926918623 29.891289161825934 22.323521467402273 62-63 22.17346185579333 25.496454093374116 30.043146973696565 22.286937077135985 64-65 22.274268674047 25.089666938067133 29.963776186976038 22.672288200909826 66-67 22.364717917512042 25.587209383552395 29.54504176556332 22.503030933372244 68-69 22.36714139683836 25.4815927897105 29.80034632612558 22.350919487325566 70-71 22.37594682931032 25.373744837505114 29.772372880961267 22.477935452223296 72-73 22.338154847282503 25.62713832436091 29.31087743481797 22.723829393538615 74-75 22.253337690188772 25.89563261463329 29.20961105751282 22.64141863766512 76-77 22.436253191124386 25.849097170853323 29.153118860796734 22.56153077722556 78-79 22.341830069925184 25.76295602917469 29.230264123001266 22.664949777898862 80-81 22.309669727846803 26.16649280653714 29.06312675024372 22.46071071537234 82-83 22.539198116099847 25.78363245319162 28.998106941138214 22.679062489570327 84-85 22.38527709453759 25.67893032140188 28.91219796536289 23.023594618697643 86-87 22.36740795474655 25.674796722726384 29.14696504469151 22.81083027783556 88-89 22.3664847344597 25.83165682494148 28.828958888288657 22.972899552310167 90-91 22.262625854944318 26.024768936065847 29.014551517089572 22.698053691900267 92-93 22.337466178404163 25.502686323423717 29.011827274293722 23.148020223878397 94-95 22.28013337762696 25.541303288180746 29.088027370976704 23.090535963215594 96-97 22.437359222215843 25.55919147951921 28.9388673199946 23.06458197827035 98-99 22.317508956901317 25.586545088761802 28.749091891287744 23.346854063049133 100-101 22.142630946578205 25.376206546475128 29.133537412642774 23.34762509430389 102-103 22.446118883072856 24.944349235325895 29.14097572788406 23.46855615371719 104-105 22.211873404988545 25.314966252507 29.110472589087795 23.36268775341666 106-107 22.322838832060814 25.253087382770268 28.88661731821678 23.53745646695214 108-109 22.577998691386984 25.260422528324177 28.559508905321152 23.602069874967693 110-111 22.139457556395882 25.605328192040933 28.799076678526408 23.456137573036777 112-113 22.145934072757473 25.323155255784354 28.97329224846514 23.557618422993038 114-115 21.949405916924615 25.48782494496931 28.638390154396404 23.924378983709673 116-117 21.933928032269908 25.521913902931036 28.84670164073882 23.69745642406024 118-119 21.886513912357078 25.6765242120574 28.501684664623284 23.935277210962234 120-121 21.78626037147137 25.867089933948574 28.594680182242953 23.751969512337105 122-123 21.794443909753895 25.787185226810553 28.47830001521281 23.940070848222742 124-125 21.890013986535052 25.800189695292236 28.39220044986658 23.917595868306137 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 639.0 1 445.5 2 653.5 3 999.0 4 568.0 5 317.0 6 936.0 7 2660.0 8 4743.5 9 5074.0 10 3181.5 11 1439.0 12 1056.5 13 1085.5 14 1156.5 15 1208.0 16 1269.5 17 1342.5 18 1434.0 19 1494.5 20 1495.5 21 1607.5 22 1739.0 23 1946.5 24 2246.0 25 2623.0 26 3104.5 27 3582.5 28 4103.0 29 4981.0 30 6044.0 31 7137.5 32 8723.0 33 9845.0 34 10670.5 35 11625.0 36 13194.5 37 14653.0 38 15113.0 39 15407.5 40 15577.0 41 16880.5 42 17653.5 43 18178.5 44 20081.0 45 21674.0 46 22188.0 47 22665.0 48 23494.0 49 23447.5 50 23409.5 51 23111.5 52 22667.5 53 23316.5 54 23670.0 55 22389.5 56 20070.0 57 18125.0 58 16926.5 59 15426.0 60 13427.5 61 11230.0 62 9099.0 63 7046.0 64 5394.5 65 4253.5 66 3093.5 67 2489.0 68 1809.0 69 1047.5 70 772.5 71 570.0 72 468.5 73 405.5 74 318.5 75 267.5 76 179.5 77 83.0 78 38.5 79 13.5 80 5.0 81 3.5 82 2.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018783103279126228 2 0.0018627871020621054 3 3.1046451701035086E-4 4 0.14824680687244254 5 0.04253363883041807 6 0.013194741972939912 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.5523225850517543E-4 30-31 7.761612925258771E-5 32-33 1.5523225850517543E-4 34-35 4.6569677551552634E-4 36-37 0.0012418580680414034 38-39 0.0035703419456190348 40-41 0.0 42-43 0.001397090326546579 44-45 0.0010866258095362281 46-47 7.761612925258771E-5 48-49 0.0017851709728095174 50-51 0.0023284838775776318 52-53 1.5523225850517543E-4 54-55 0.0 56-57 0.0018627871020621054 58-59 0.0024061000068302195 60-61 0.0015523225850517545 62-63 3.8808064626293863E-4 64-65 0.0010090096802836403 66-67 7.761612925258773E-4 68-69 8.537774217784649E-4 70-71 0.0010090096802836403 72-73 0.0 74-75 7.761612925258771E-5 76-77 0.004113654850387149 78-79 0.0011642419387888159 80-81 0.0 82-83 7.761612925258771E-5 84-85 3.8808064626293863E-4 86-87 3.1046451701035086E-4 88-89 0.0 90-91 3.1046451701035086E-4 92-93 0.0010866258095362281 94-95 0.0015523225850517545 96-97 0.0010866258095362281 98-99 0.0 100-101 3.1046451701035086E-4 102-103 0.0 104-105 0.0 106-107 2.3284838775776317E-4 108-109 3.8808064626293863E-4 110-111 0.0 112-113 1.5523225850517543E-4 114-115 0.0015523225850517545 116-117 6.985451632732895E-4 118-119 3.8808064626293863E-4 120-121 1.5523225850517543E-4 122-123 3.1046451701035086E-4 124-125 7.761612925258773E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 644196.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.923178786712 #Duplication Level Percentage of deduplicated Percentage of total 1 70.3584527984226 33.01442259816821 2 14.974200829848028 14.052742054541802 3 5.770189014052325 8.12266832218496 4 2.8711748256214085 5.3889859868216 5 1.5225141985747017 3.5720602972514137 6 1.0130022826787382 2.851997233288908 7 0.6823264587627494 2.2411848490799997 8 0.48447366026031197 1.8186435342277911 9 0.34591621786804044 1.4608339682620755 >10 1.804971931320117 15.290795940994256 >50 0.11805929764786387 3.7620282586643903 >100 0.05173384030989607 4.467563538350779 >500 6.632543629473048E-4 0.19217836885624648 >1k 0.0016581359073682622 1.4499741222692346 >5k 6.632543629473048E-4 2.3139209270383687 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8525 1.3233550037566206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6345 0.9849486802153381 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4257 0.6608237244565319 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1502 0.2331588522747735 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1409 0.2187222522337922 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1144 0.17758570372992072 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1006 0.1561636520562065 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 662 0.10276375513042614 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.761612925258773E-5 0.0 22-23 0.0 0.0 0.0 3.8808064626293863E-4 0.0 24-25 0.0 0.0 0.0 0.0012418580680414034 0.0 26-27 0.0 0.0 0.0 0.0018627871020621052 0.0 28-29 0.0 0.0 0.0 0.003182261299356097 0.0 30-31 0.0 0.0 0.0 0.007683996796006185 0.0 32-33 0.0 0.0 0.0 0.015445609721264957 0.0 34-35 0.0 0.0 0.0 0.024992393619333246 0.0 36-37 0.0 0.0 0.0 0.04222317431340772 0.0 38-39 0.0 0.0 0.0 0.06853504213003496 0.0 40-41 0.0 0.0 0.0 0.10322945190594167 0.0 42-43 0.0 0.0 0.0 0.1371477003893225 0.0 44-45 0.0 0.0 0.0 0.16912554564138865 0.0 46-47 0.0 0.0 0.0 0.2130562747983533 0.0 48-49 0.0 0.0 0.0 0.2636619910710405 0.0 50-51 0.0 0.0 0.0 0.3252891976975951 0.0 52-53 0.0 0.0 0.0 0.37985333656216436 0.0 54-55 0.0 0.0 0.0 0.4380654335016051 0.0 56-57 0.0 0.0 0.0 0.5049705369173357 0.0 58-59 0.0 0.0 0.0 0.5819657371359027 0.0 60-61 0.0 0.0 0.0 0.6657911567286974 0.0 62-63 0.0 0.0 0.0 0.7523331408453328 0.0 64-65 0.0 0.0 0.0 0.8393408217374836 0.0 66-67 0.0 0.0 0.0 0.9414060317046364 0.0 68-69 0.0 0.0 0.0 1.0725772901415098 0.0 70-71 0.0 0.0 0.0 1.2137610292519667 0.0 72-73 0.0 0.0 0.0 1.3482697812467013 0.0 74-75 0.0 0.0 0.0 1.487047420350328 0.0 76-77 0.0 0.0 0.0 1.6370017820663276 0.0 78-79 0.0 0.0 0.0 1.8219610180752444 0.0 80-81 0.0 0.0 0.0 2.0364143832001442 0.0 82-83 0.0 0.0 0.0 2.259948835447597 0.0 84-85 0.0 0.0 0.0 2.469667616688089 0.0 86-87 0.0 0.0 0.0 2.685983768915051 0.0 88-89 0.0 0.0 0.0 2.930862656706965 0.0 90-91 0.0 0.0 0.0 3.2184304155878025 0.0 92-93 0.0 0.0 0.0 3.5160882712714763 0.0 94-95 0.0 0.0 0.0 3.823215294723966 0.0 96-97 0.0 0.0 0.0 4.121649311700166 0.0 98-99 0.0 0.0 0.0 4.460133251370701 0.0 100-101 0.0 0.0 0.0 4.852017087967017 0.0 102-103 0.0 0.0 0.0 5.25166253748859 0.0 104-105 0.0 0.0 0.0 5.648979503132587 0.0 106-107 0.0 0.0 0.0 6.054523778477358 0.0 108-109 0.0 0.0 0.0 6.477764531291719 0.0 110-111 0.0 0.0 0.0 6.967522306875547 0.0 112-113 0.0 0.0 0.0 7.4837471825345085 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2430 0.0 76.542015 1 GTATCAA 4710 0.0 59.361313 1 TATAACG 65 5.419988E-7 55.00733 2 ATCAACG 6045 0.0 45.740864 3 TATCAAC 6090 0.0 45.402878 2 TCAACGC 6110 0.0 45.254257 4 CAACGCA 6195 0.0 44.677425 5 AACGCAG 6355 0.0 43.63269 6 GTGGTAT 945 0.0 42.897175 1 TAACGCA 85 3.442021E-6 42.06443 4 TGGTATC 970 0.0 39.317955 2 ACGCAGA 7180 0.0 38.447468 7 CGCAGAG 7185 0.0 38.337906 8 ATCACGC 95 7.3890114E-6 37.636593 3 GTGTACG 65 0.0019200643 36.685818 1 GCAGAGT 7630 0.0 35.946 9 TATCACG 110 2.0171246E-5 32.50433 2 ATAACGC 115 2.7333199E-5 31.091099 3 GTATAAC 155 1.9356594E-7 30.76875 1 GAGTACT 5560 0.0 30.6566 12-13 >>END_MODULE