FastQCFastQC Report
Fri 27 May 2016
SRR940050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159633
Sequences flagged as poor quality0
Sequence length125
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87895.505753822831118No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17821.1163105372949202No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12080.7567357626555913No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11230.703488627038269No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8840.5537702104201512No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6180.3871379977824134No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5180.32449430882085784No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4740.2969310856777734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.27751154209969114No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3440.21549429002775114No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3340.20922992113159558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3270.2048448629042867No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2640.1653793388585067No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.1597414068519667No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2430.15222416417658002No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2360.14783910594927113No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT2280.14282761083234669No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.12278163036464891No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.11964944591657113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.11651726146849335No Hit
ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.1115057663515689No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.10461496056579779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA850.077.2600862
GAACAAA7050.071.9799041
CATGATA300.00417373959.7009742
TACAAGG701.29675755E-859.700972
GTACAAG1850.051.6332741
TAGGGAG350.00773842251.07585
AGAACAA3450.050.1834342
GAGAACA5200.048.2200161
GTCACTA505.2309566E-447.760782
ACGCAAA1056.1390892E-945.4864581
GGAAACA2250.045.1074031
CGCAAAA1109.262294E-943.418892
ATATGGG558.431196E-443.3506663
TAACAAC1800.043.117371
GTATCAA11000.042.333421
GTACATA757.996322E-539.800651
GCAGAGA3250.038.51543
GTACATG20850.038.3689771
GAAAACA5300.038.298741
TACATGG20800.037.6001322
Produced by FastQC (version 0.11.5)