##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940050_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 159633 Sequences flagged as poor quality 0 Sequence length 125 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.937224759291624 31.0 15.0 34.0 7.0 34.0 2 25.15207381932307 31.0 16.0 34.0 7.0 34.0 3 25.79689036727995 31.0 22.0 34.0 7.0 34.0 4 27.19033658454079 35.0 17.0 37.0 7.0 37.0 5 29.295559188889516 35.0 28.0 37.0 7.0 37.0 6 27.800680310462123 37.0 17.0 37.0 7.0 37.0 7 28.484185600721656 37.0 23.0 37.0 7.0 37.0 8 27.403412828174627 37.0 15.0 37.0 7.0 37.0 9 27.78950467635138 38.0 13.0 39.0 7.0 39.0 10-11 28.65883620554647 38.0 15.5 39.0 7.0 39.0 12-13 28.48489347440692 38.0 15.0 39.0 2.0 39.0 14-15 29.40575256995734 39.0 15.0 41.0 2.0 41.0 16-17 29.728336246264867 39.0 17.5 41.0 2.0 41.0 18-19 29.573916420790187 38.0 18.0 41.0 2.0 41.0 20-21 29.633982321950974 38.0 20.0 41.0 2.0 41.0 22-23 29.87166813879336 38.0 24.0 41.0 2.0 41.0 24-25 30.2479217956187 38.0 26.5 41.0 2.0 41.0 26-27 30.41850682502991 38.0 27.0 41.0 2.0 41.0 28-29 30.30285404646909 38.0 27.0 41.0 2.0 41.0 30-31 30.15558186590492 37.0 27.0 41.0 2.0 41.0 32-33 30.061133976057583 36.5 27.0 41.0 2.0 41.0 34-35 29.834570546190324 36.0 25.0 41.0 2.0 41.0 36-37 29.52034667017471 36.0 25.0 41.0 2.0 41.0 38-39 29.269073437196568 35.0 25.0 41.0 2.0 41.0 40-41 29.11119254790676 35.0 25.0 41.0 2.0 41.0 42-43 28.80260347171324 35.0 25.0 41.0 2.0 41.0 44-45 28.676999743160877 35.0 25.0 41.0 2.0 41.0 46-47 28.513969542638428 35.0 25.0 41.0 2.0 41.0 48-49 28.320594739183 35.0 25.0 41.0 2.0 41.0 50-51 28.152189710147653 35.0 25.0 40.0 2.0 41.0 52-53 28.135611057863976 35.0 25.0 41.0 2.0 41.0 54-55 27.925313061835585 35.0 23.5 41.0 2.0 41.0 56-57 27.771184529514574 35.0 22.5 40.5 2.0 41.0 58-59 27.49394235527742 35.0 13.0 40.0 2.0 41.0 60-61 27.177973852524225 35.0 2.0 40.0 2.0 41.0 62-63 26.81893468142552 35.0 2.0 39.5 2.0 41.0 64-65 26.48569844581008 34.5 2.0 39.0 2.0 41.0 66-67 26.172542644691262 34.0 2.0 39.0 2.0 41.0 68-69 25.862512763651626 34.0 2.0 37.5 2.0 41.0 70-71 25.54133857034573 34.0 2.0 37.0 2.0 40.5 72-73 25.220568428833637 33.5 2.0 36.5 2.0 40.0 74-75 24.922443980881148 33.0 2.0 36.0 2.0 39.0 76-77 24.608470679621384 33.0 2.0 35.5 2.0 39.0 78-79 24.308382352019947 33.0 2.0 35.0 2.0 38.5 80-81 24.016318680974486 33.0 2.0 35.0 2.0 37.0 82-83 23.741447570364524 33.0 2.0 35.0 2.0 37.0 84-85 23.48215281301485 32.5 2.0 35.0 2.0 36.5 86-87 23.25253237112627 32.0 2.0 35.0 2.0 36.0 88-89 23.05430894614522 32.0 2.0 35.0 2.0 36.0 90-91 22.873907650673733 32.0 2.0 35.0 2.0 35.5 92-93 22.68608621024475 31.0 2.0 35.0 2.0 35.0 94-95 22.53491446004272 31.0 2.0 35.0 2.0 35.0 96-97 22.40308081662313 31.0 2.0 35.0 2.0 35.0 98-99 22.29092355590636 30.5 2.0 35.0 2.0 35.0 100-101 22.161273045047075 30.0 2.0 35.0 2.0 35.0 102-103 21.667728477194565 29.5 2.0 34.5 2.0 35.0 104-105 22.181206266874643 31.0 2.0 35.0 2.0 35.0 106-107 22.181688623279648 31.0 2.0 35.0 2.0 35.0 108-109 22.123495768418813 31.0 2.0 35.0 2.0 35.0 110-111 22.01922848032675 30.0 2.0 35.0 2.0 35.0 112-113 21.908505760087202 30.0 2.0 35.0 2.0 35.0 114-115 21.778210019231615 30.0 2.0 35.0 2.0 35.0 116-117 21.628751573922685 30.0 2.0 35.0 2.0 35.0 118-119 21.472521345837013 29.0 2.0 35.0 2.0 35.0 120-121 21.29799602839012 29.0 2.0 35.0 2.0 35.0 122-123 21.1067260528838 29.0 2.0 35.0 2.0 35.0 124-125 20.214955554302684 26.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28480297965545986 1101 2 -0.11936386379340647 1101 3 -0.24719643704997196 1101 4 -0.07491927811830124 1101 5 -0.8979888278211483 1101 6 -0.26438802408416606 1101 7 0.15030311713760014 1101 8 0.2820334189914995 1101 9 -0.5597271686980818 1101 10-11 0.07611802410031743 1101 12-13 -0.2977837295839336 1101 14-15 0.11305254662607211 1101 16-17 -0.059986205023520256 1101 18-19 -0.014167494471191588 1101 20-21 -0.37411744624547083 1101 22-23 -0.11975979425808703 1101 24-25 -0.02002156758202389 1101 26-27 0.408151323719391 1101 28-29 0.5585529845581867 1101 30-31 0.393671331814911 1101 32-33 0.6101478086523286 1101 34-35 0.23682045388366646 1101 36-37 0.04841435399546157 1101 38-39 0.04443946025400081 1101 40-41 0.3708076106382556 1101 42-43 0.2523828439077107 1101 44-45 0.23041825749681166 1101 46-47 0.15733649156490515 1101 48-49 -0.01289391464622014 1101 50-51 0.08473614308777755 1101 52-53 -0.22323634921165336 1101 54-55 -0.15878568595936926 1101 56-57 -0.1444556928002001 1101 58-59 -0.043577877606686855 1101 60-61 0.1672603823067682 1101 62-63 0.2444137623910727 1101 64-65 0.31597749907251327 1101 66-67 0.07637185703527294 1101 68-69 0.12322643077613904 1101 70-71 -0.037028888995291 1101 72-73 -0.10773148620903328 1101 74-75 -0.3047097371088121 1101 76-77 -0.2040791987451449 1101 78-79 0.131061972366183 1101 80-81 0.013783276741754946 1101 82-83 0.20301938847481438 1101 84-85 0.3051688378312285 1101 86-87 0.286110711744346 1101 88-89 0.03626687879820878 1101 90-91 0.034050726396699815 1101 92-93 -0.23332455186417533 1101 94-95 -0.044660193398033954 1101 96-97 -0.40123701811201684 1101 98-99 -0.20386504783376935 1101 100-101 -0.368480322590063 1101 102-103 -0.4535222893187836 1101 104-105 -0.42877649848932364 1101 106-107 -0.3758923188000125 1101 108-109 -0.24965513009505713 1101 110-111 -0.5790737677062339 1101 112-113 -0.12888222589919707 1101 114-115 -0.21932058815051292 1101 116-117 -0.27907274337605514 1101 118-119 -0.19742540027487365 1101 120-121 -0.010174781660321486 1101 122-123 -0.2648924040563507 1101 124-125 -0.10081061946267411 1102 1 -0.06408528378388922 1102 2 -0.29532783789979433 1102 3 -0.5633517987161696 1102 4 -0.2760169420642633 1102 5 -0.222362596467363 1102 6 -1.4009205318241058 1102 7 -0.35107599910219633 1102 8 -0.24574592241012994 1102 9 -1.5723080862325602 1102 10-11 -1.341571252567288 1102 12-13 -1.1532439040843556 1102 14-15 -1.5112649315613744 1102 16-17 -1.4784776207285617 1102 18-19 -1.8472099937675601 1102 20-21 -1.365054682383942 1102 22-23 -1.2310601038556115 1102 24-25 -0.5780148127269698 1102 26-27 -0.6215841111244025 1102 28-29 -0.4335382059850126 1102 30-31 -0.35753891304858243 1102 32-33 -0.07465376748051966 1102 34-35 -0.05929550446139231 1102 36-37 -0.49160816218804726 1102 38-39 -0.3793573311898477 1102 40-41 -0.25591628466149174 1102 42-43 -0.2421429089771756 1102 44-45 -0.06619024236246318 1102 46-47 0.0228304403405879 1102 48-49 -0.06486408070307448 1102 50-51 -0.28901843051199805 1102 52-53 -0.27636046967323225 1102 54-55 -0.4437139156244392 1102 56-57 -0.6537576911114868 1102 58-59 -0.5604650152368578 1102 60-61 -1.0194691420388544 1102 62-63 -0.725161244645232 1102 64-65 -0.7486017297482022 1102 66-67 -1.1790601722357614 1102 68-69 -1.1627431723761248 1102 70-71 -1.0795985484380175 1102 72-73 -1.2798817817339412 1102 74-75 -1.2948307615951613 1102 76-77 -1.3980701922717387 1102 78-79 -1.3831091506284707 1102 80-81 -1.4478547756083735 1102 82-83 -1.4125871412184026 1102 84-85 -1.4014171458445404 1102 86-87 -1.4537710782922417 1102 88-89 -1.8713041879059844 1102 90-91 -1.804409729556017 1102 92-93 -1.5633836568458825 1102 94-95 -1.8180629516305125 1102 96-97 -1.380311040065294 1102 98-99 -1.5853314142846564 1102 100-101 -1.7550128303300028 1102 102-103 -1.4427567390795488 1102 104-105 -1.7516444973635181 1102 106-107 -1.6253012689829553 1102 108-109 -1.380277139664436 1102 110-111 -1.1361522928962167 1102 112-113 -1.1836528422797237 1102 114-115 -1.5585128200672003 1102 116-117 -1.57033364965244 1102 118-119 -1.5349852088873135 1102 120-121 -1.497002814308786 1102 122-123 -1.2454862683963448 1102 124-125 -1.5053279287787475 1103 1 -0.4957473810177966 1103 2 -0.3945976334968506 1103 3 -0.5790959828688322 1103 4 -0.7648738600044886 1103 5 -0.7565247615641333 1103 6 -0.06858252047743463 1103 7 -0.19097393335665558 1103 8 0.1176732800655067 1103 9 0.569661022592328 1103 10-11 -0.09942298578480191 1103 12-13 0.15788017637385465 1103 14-15 -0.00669097461357282 1103 16-17 -0.0027860981573724075 1103 18-19 -0.07769942073354841 1103 20-21 -0.7325999469133819 1103 22-23 -1.049214776892338 1103 24-25 -0.8272751075232456 1103 26-27 -1.1258187537585655 1103 28-29 -1.1154785409561043 1103 30-31 -1.0964569075652655 1103 32-33 -1.412855130166804 1103 34-35 -1.320513235670166 1103 36-37 -1.1573564181124745 1103 38-39 -1.1903321133500597 1103 40-41 -1.0278699207536768 1103 42-43 -0.8265778827821109 1103 44-45 -1.15492772166429 1103 46-47 -1.1005849618147359 1103 48-49 -1.1414405350042216 1103 50-51 -1.1661187861133975 1103 52-53 -1.3424588979693333 1103 54-55 -1.352574090982074 1103 56-57 -1.0180998285202136 1103 58-59 -0.6965300550044944 1103 60-61 -0.47752989287356584 1103 62-63 -0.8263583455437349 1103 64-65 -0.8370949027442123 1103 66-67 -0.6734411272019685 1103 68-69 -0.5851759202791627 1103 70-71 -0.5527248547981252 1103 72-73 -0.6469112885066508 1103 74-75 -0.7098393123158644 1103 76-77 -0.563750585333441 1103 78-79 -0.37477559108559433 1103 80-81 -0.26492364284146674 1103 82-83 -0.6204510897512066 1103 84-85 -0.5198378413031541 1103 86-87 -0.7028820747905193 1103 88-89 -0.6320740242207599 1103 90-91 -0.4701036951902644 1103 92-93 -0.4870515088505485 1103 94-95 -0.2259051840472459 1103 96-97 -0.09056376136609146 1103 98-99 -0.4291789671498272 1103 100-101 -0.1181062910645494 1103 102-103 -0.1601079158215839 1103 104-105 -0.2178895094431077 1103 106-107 0.004778266292159827 1103 108-109 -0.026331592825485473 1103 110-111 -0.1277780156357018 1103 112-113 -0.05917877946585648 1103 114-115 0.008264236856167173 1103 116-117 -0.16695679360314486 1103 118-119 -0.29409919135154894 1103 120-121 -0.22462842846769604 1103 122-123 0.05422327855118425 1103 124-125 -0.03902862640897098 1104 1 0.5444866716440515 1104 2 0.9968404400697004 1104 3 0.3087537833285623 1104 4 0.22207320308470457 1104 5 1.2014407806828729 1104 6 0.25106440007969155 1104 7 1.338895287217973 1104 8 0.6416911840939115 1104 9 0.6037937024452944 1104 10-11 0.9068284207816788 1104 12-13 0.7722058996615928 1104 14-15 0.9475612321329443 1104 16-17 1.0029902015100518 1104 18-19 1.2569758812093887 1104 20-21 1.118441537420594 1104 22-23 1.1611450540696282 1104 24-25 1.2960401384070899 1104 26-27 1.3314466309529926 1104 28-29 1.4120401507495295 1104 30-31 1.3389785654161592 1104 32-33 1.4258335747918132 1104 34-35 1.5346166685582858 1104 36-37 1.5456142502879153 1104 38-39 1.493946849416563 1104 40-41 1.4168926508249307 1104 42-43 1.8036014075582187 1104 44-45 1.934942499135392 1104 46-47 1.9011529292107454 1104 48-49 1.5861856808943564 1104 50-51 1.2195429877857293 1104 52-53 1.2663280791005072 1104 54-55 1.4293268367266592 1104 56-57 1.5790470295228474 1104 58-59 1.3785118294195016 1104 60-61 0.9446651010000551 1104 62-63 1.1837189218414252 1104 64-65 1.168181284397896 1104 66-67 1.159221221826563 1104 68-69 1.1251839106828037 1104 70-71 1.1665619847407704 1104 72-73 1.1369275752376886 1104 74-75 1.3405845330493413 1104 76-77 0.9348240939694037 1104 78-79 0.9125890659622371 1104 80-81 0.8327358305400452 1104 82-83 0.4149068657887902 1104 84-85 0.3161877644581388 1104 86-87 0.06632849758826964 1104 88-89 0.33069259826930164 1104 90-91 -0.026216320530966897 1104 92-93 0.1359261611251945 1104 94-95 0.1649327544889232 1104 96-97 0.16219310892972771 1104 98-99 0.33446785338479756 1104 100-101 0.09401643654916114 1104 102-103 0.06754330570325351 1104 104-105 0.1441169420084698 1104 106-107 0.19194537889249474 1104 108-109 0.1091989015357413 1104 110-111 0.03793426962848301 1104 112-113 0.06183594884801735 1104 114-115 -0.07059100557338027 1104 116-117 -0.18292288597393025 1104 118-119 -0.058496180674147524 1104 120-121 0.04069376902674193 1104 122-123 0.10756027937637214 1104 124-125 -0.08622674598587565 1105 1 -0.4502406595934154 1105 2 -0.48762622824725455 1105 3 -0.7529092114482765 1105 4 -0.45775450689375674 1105 5 -0.12425172123558781 1105 6 -0.3367762811590147 1105 7 -0.23525625868889932 1105 8 -1.3557343673084183 1105 9 -0.37350659213103654 1105 10-11 -1.2313006482085953 1105 12-13 -1.0168130868968106 1105 14-15 -0.9812082309459988 1105 16-17 -1.327455128588717 1105 18-19 -0.781319041680927 1105 20-21 -1.4323295594430334 1105 22-23 -1.1678026400660784 1105 24-25 -1.3213571930779224 1105 26-27 -1.4326447619722238 1105 28-29 -1.5014311466240393 1105 30-31 -1.3127158673387491 1105 32-33 -1.3311904616465355 1105 34-35 -1.3881993821385485 1105 36-37 -1.7357432762610863 1105 38-39 -1.677922348791224 1105 40-41 -1.518176785024263 1105 42-43 -1.4087782245926839 1105 44-45 -1.3479869263169952 1105 46-47 -1.4961414243303608 1105 48-49 -1.3487971148578062 1105 50-51 -1.2183979484224032 1105 52-53 -1.0759869442923211 1105 54-55 -0.9937367571045677 1105 56-57 -0.7363229236914997 1105 58-59 -0.928132228307561 1105 60-61 -0.8013061344877279 1105 62-63 -0.9117089564663701 1105 64-65 -0.7984499010861725 1105 66-67 -0.9522845830599387 1105 68-69 -1.1535125271714932 1105 70-71 -0.8567194470487145 1105 72-73 -1.0043329144026316 1105 74-75 -1.2295444369236783 1105 76-77 -1.4662452923711662 1105 78-79 -1.5576314949705043 1105 80-81 -1.1245134701505997 1105 82-83 -1.0791149675156646 1105 84-85 -1.3486264544194988 1105 86-87 -1.3316094681022541 1105 88-89 -1.2293242346638387 1105 90-91 -1.2278773349627308 1105 92-93 -1.2124041604333335 1105 94-95 -1.0578313121496876 1105 96-97 -1.1160082426557878 1105 98-99 -1.0958116228139332 1105 100-101 -1.0074514942377775 1105 102-103 -1.1823638656213475 1105 104-105 -1.4055154564369552 1105 106-107 -1.1577092984350301 1105 108-109 -1.2155900679421876 1105 110-111 -1.3402745082510634 1105 112-113 -1.240810287258629 1105 114-115 -1.3535575624959222 1105 116-117 -1.3097392337225244 1105 118-119 -1.36641508554332 1105 120-121 -1.5875088202744436 1105 122-123 -1.5482433694094304 1105 124-125 -1.354910064054053 1106 1 -0.4608518551058616 1106 2 -0.1369893227897343 1106 3 -0.12932618001202556 1106 4 -0.9971958999053285 1106 5 -0.6941944581761028 1106 6 -0.5538617082946935 1106 7 -0.685437396938287 1106 8 -0.09103879643077661 1106 9 -0.35691199121950135 1106 10-11 -0.19101783881277967 1106 12-13 0.1817144344307522 1106 14-15 -0.2920882122478581 1106 16-17 0.04845932741221759 1106 18-19 -0.11110751895099824 1106 20-21 -0.31034755640458656 1106 22-23 -0.16266065105123317 1106 24-25 -0.16402401556243618 1106 26-27 -0.43989029194768037 1106 28-29 0.06246975322403969 1106 30-31 0.12531147869373527 1106 32-33 0.2162677596927196 1106 34-35 -0.04249411159980809 1106 36-37 -0.06847671087601981 1106 38-39 -0.18695588858320988 1106 40-41 -0.030844776142647135 1106 42-43 -0.09969793944602401 1106 44-45 -0.09803951484100892 1106 46-47 0.0043377155551134194 1106 48-49 -0.03155976531941462 1106 50-51 0.3123371222799456 1106 52-53 0.48196560917267917 1106 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122-123 -1.2173854788485947 2216 124-125 -1.4020571568587954 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13386.0 3 4050.0 4 4667.0 5 2990.0 6 2947.0 7 2495.0 8 1961.0 9 1616.0 10 2021.0 11 2435.0 12 2241.0 13 1822.0 14 1603.0 15 1449.0 16 1428.0 17 1236.0 18 1032.0 19 805.0 20 636.0 21 520.0 22 662.0 23 2072.0 24 4886.0 25 3364.0 26 5153.0 27 2568.0 28 1555.0 29 1625.0 30 1418.0 31 1754.0 32 2365.0 33 3020.0 34 4496.0 35 9583.0 36 14590.0 37 30688.0 38 18396.0 39 98.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51276073004412 29.156011833132773 10.54264440433213 15.788583032490974 2 11.965726122846112 37.72323530334892 30.938765261605095 19.372273312199866 3 15.800389746288952 47.53087580126699 19.846357250722157 16.822377201721903 4 8.8626486984768 33.10716323753503 25.482388205710272 32.547799858277884 5 9.317727554683927 57.74472271129518 20.421180583838243 12.516369150182646 6 19.985585359739282 50.563424417147154 13.435698169967411 16.01529205314615 7 20.017546608177973 54.49161836127213 12.394798684004387 13.096036346545512 8 19.49937924029044 52.223448414241105 13.043478260869565 15.23369408459889 9 20.49236365917145 39.84695957600276 12.041897952143508 27.618778812682283 10-11 17.294446498743397 50.01942879347192 15.672142243837625 17.013982463947052 12-13 18.68528155753499 48.622838541095525 16.92102352657353 15.770856374795954 14-15 18.046323386606687 46.62661245356071 17.187705570208685 18.139358589623914 16-17 16.326313217076365 48.733621991490644 17.905369417691695 17.0346953737413 18-19 16.947983979642363 46.193284737412796 21.174951581665592 15.683779701279246 20-21 20.209047206637294 43.57357131202069 20.636743608088263 15.580637873253755 22-23 19.51952140268145 44.4370586833557 20.063524513062344 15.979895400900507 24-25 18.04427142104867 43.72414052508839 21.256801825522203 16.97478622834073 26-27 17.77387251078386 43.57964708498967 21.544856794694685 17.101623609531774 28-29 17.857646273888246 43.35232594198223 21.54632619883664 17.243701585292893 30-31 17.90243917718882 43.68279106773125 21.442957960450684 16.97181179462924 32-33 17.18669243563761 43.751389989569205 21.45955952600964 17.602358048783547 34-35 17.39355153975548 43.37314676464968 22.176175216687184 17.05712647890765 36-37 17.531072242964893 43.305685719297365 21.543526198411307 17.61971583932643 38-39 17.563774870946723 43.38069463238611 21.765837217461034 17.289693279206134 40-41 17.522944400939704 43.43523884103367 21.9912294440094 17.050587314017225 42-43 17.145828504125447 43.74353930296518 21.70574931555767 17.404882877351692 44-45 17.20367121914547 43.656809923568474 21.850645282546047 17.28887357474001 46-47 16.897966855674948 44.237649196453745 21.66442154067855 17.199962407192757 48-49 16.70660764799308 43.934766196459506 21.474464399271977 17.884161756275432 50-51 16.90269672565069 44.289074956380645 21.394629102333987 17.413599215634683 52-53 17.096863116084126 44.130637714027955 21.15407506719209 17.61842410269583 54-55 16.97840735020941 43.97438829179053 21.303828912786745 17.74337544521331 56-57 17.25533561178236 44.18791879031273 20.960269153538434 17.596476444366477 58-59 16.88375560734782 44.03866877177155 20.987457083427312 18.09011853745332 60-61 16.41680240571357 44.43835358977572 21.184375391554944 17.960468612955772 62-63 16.620302494893675 44.56160795960052 20.78931869729208 18.028770848213725 64-65 16.244430937357052 44.891187878711925 20.704067373908902 18.160313810022117 66-67 16.415866958665827 44.9315274501142 20.361301965354865 18.291303625865112 68-69 16.67726744349995 44.711256771703134 20.386077159033587 18.225398625763333 70-71 15.973984473392358 44.94006779576058 20.576201307042112 18.50974642380496 72-73 16.098867534420375 45.21592030449697 20.199865294550694 18.485346866531962 74-75 15.873637388798395 45.541598797143216 20.365555694775093 18.219208119283298 76-77 15.649452759383282 45.64964070693339 20.67109805223689 18.029808481446445 78-79 15.858877637976839 45.55867462028172 20.896410847661535 17.686036894079905 80-81 15.824989661783686 45.80299745617223 20.62944073382539 17.742572148218695 82-83 15.763125495368211 45.70795753225963 20.268039234728562 18.2608777376436 84-85 16.1911713568768 45.518168233550846 20.492401577639868 17.798258831932483 86-87 16.287426650500798 45.4508429122375 20.38058717194407 17.881143265317633 88-89 16.613353468073175 45.009602641744706 20.360733497711344 18.01631039247078 90-91 16.6997719183919 45.14762644744098 20.136974284425285 18.01562734974184 92-93 16.605934898468806 44.97889792989789 20.196576670854704 18.2185905007786 94-95 16.27359081681245 44.90024812651946 20.41435875585854 18.411802300809544 96-97 16.467351970850032 45.32093477913509 19.862020133029638 18.349693116985247 98-99 16.12796089735556 45.33776162426369 20.043238501065296 18.49103897731545 100-101 15.66411132797203 46.1796798882209 20.191352783982506 17.96485599982456 102-103 15.46188262128257 46.76632035391576 19.97543644179314 17.796360583008536 104-105 15.03145521078751 47.66780289738834 19.88727505138102 17.413466840443128 106-107 14.262260154654033 48.84761439260067 19.634733240591043 17.255392212154252 108-109 14.25098373392817 49.85400636607434 18.88142559963909 17.013584300358406 110-111 14.031592072532693 50.8045264008722 18.656992299354 16.506889227241114 112-113 14.137436244470344 51.13287466947379 18.370051505695702 16.359637580360165 114-115 13.791612759732224 51.749404021640046 18.09831998320923 16.360663235418503 116-117 13.798374819093684 51.64522939860788 18.161114696171367 16.39528108612707 118-119 13.627419647948665 51.96235093590009 18.1204920446926 16.289737371458653 120-121 13.605205611634306 51.824282411323516 18.356234766317662 16.21427721072451 122-123 13.81982907696549 51.64970802736774 18.001115260269167 16.52934763539761 124-125 13.963345864661655 51.83302005012531 17.81265664160401 16.390977443609025 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7797.0 1 7069.5 2 6057.0 3 4812.0 4 2943.0 5 1634.0 6 1128.5 7 996.0 8 1027.5 9 991.5 10 770.5 11 603.5 12 555.5 13 534.5 14 508.0 15 521.5 16 561.5 17 564.0 18 578.5 19 638.5 20 715.0 21 814.5 22 879.0 23 978.0 24 1152.5 25 1351.0 26 1536.0 27 1719.0 28 1898.5 29 2053.0 30 2307.5 31 2505.0 32 2695.0 33 2999.5 34 3175.0 35 3128.5 36 3259.0 37 3562.0 38 3546.5 39 3394.0 40 3338.0 41 3331.0 42 3409.5 43 3348.0 44 3277.5 45 3412.0 46 3500.0 47 3591.5 48 3776.5 49 4016.0 50 4467.5 51 4304.5 52 3594.0 53 3397.5 54 3370.0 55 3272.5 56 3086.5 57 2883.5 58 2840.0 59 2693.5 60 2366.0 61 2130.5 62 1777.5 63 1116.0 64 663.0 65 561.0 66 478.5 67 362.5 68 261.5 69 185.0 70 128.0 71 96.5 72 102.0 73 118.0 74 86.5 75 55.0 76 40.5 77 33.0 78 31.0 79 16.0 80 11.5 81 9.0 82 6.0 83 4.5 84 5.0 85 4.0 86 2.5 87 1.5 88 0.5 89 1.0 90 1.0 91 0.5 92 0.5 93 1.0 94 1.0 95 0.5 96 0.5 97 0.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05074138805886001 2 0.20547129979390225 3 0.026310349363853337 4 0.10398852367618223 5 0.021298854246928893 6 0.045729892941935565 7 0.03633333959770223 8 0.09459197033194891 9 0.12403450414388002 10-11 0.04760920361078223 12-13 0.029755752256738894 14-15 0.01002299023384889 16-17 0.02756322314308445 18-19 0.053873572506937785 20-21 0.1822931348781267 22-23 0.10461496056579779 24-25 0.07329311608502001 26-27 0.012215519347503336 28-29 0.005951150451347779 30-31 0.005951150451347779 32-33 0.005951150451347779 34-35 0.008456898009810001 36-37 0.0031321844480777785 38-39 0.005637932006540001 40-41 0.005011495116924445 42-43 0.008770116454617779 44-45 0.008143679565002223 46-47 0.017540232909235558 48-49 0.014094830016350002 50-51 0.008456898009810001 52-53 0.01002299023384889 54-55 0.01158908245788778 56-57 0.013468393126734446 58-59 0.013155174681926668 60-61 0.008143679565002223 62-63 0.016913796019620002 64-65 0.02881609692231556 66-67 0.02725000469827667 68-69 0.033514373594432226 70-71 0.022551728026160005 72-73 0.015974140685196668 74-75 0.008143679565002223 76-77 0.008770116454617779 78-79 0.02568391247423778 80-81 0.019419543578082225 82-83 0.01847988824365889 84-85 0.01722701446442778 86-87 0.02349138336058334 88-89 0.02599713091904556 90-91 0.02568391247423778 92-93 0.032261499815201114 94-95 0.02317816491577556 96-97 0.027876441587892226 98-99 0.03320115514962445 100-101 0.02035919891250556 102-103 0.029442533811931116 104-105 0.02568391247423778 106-107 0.031948281370393336 108-109 0.02317816491577556 110-111 0.022551728026160005 112-113 0.024431038695006672 114-115 0.013468393126734446 116-117 0.012528737792311114 118-119 0.033827592039240004 120-121 0.022551728026160005 122-123 0.018166669798851114 124-125 0.020672417357313337 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 159633.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.388309434766 #Duplication Level Percentage of deduplicated Percentage of total 1 83.92077227641944 51.51754336509369 2 9.024858157475816 11.080415703519948 3 3.131760480019593 5.767604442690421 4 1.4255683905465528 3.5005293391717247 5 0.7377852157230907 2.264569355960234 6 0.45614106698232576 1.68010373794892 7 0.27552144985509613 1.1839657213734003 8 0.21327401118412997 1.047402479437209 9 0.13367892567043554 0.7385690928567401 >10 0.5969631413527082 6.264368896155557 >50 0.04796114127107229 2.0503279397117136 >100 0.02857259479978775 3.556908659237125 >500 0.0030613494428344016 1.2654025170234224 >1k 0.0030613494428344016 2.5765349269887805 >5k 0.001020449814278134 5.505753822831118 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8789 5.505753822831118 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1782 1.1163105372949202 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1208 0.7567357626555913 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1123 0.703488627038269 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 884 0.5537702104201512 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 618 0.3871379977824134 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 518 0.32449430882085784 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 474 0.2969310856777734 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 443 0.27751154209969114 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 344 0.21549429002775114 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 334 0.20922992113159558 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 327 0.2048448629042867 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 264 0.1653793388585067 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 255 0.1597414068519667 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 243 0.15222416417658002 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 236 0.14783910594927113 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 228 0.14282761083234669 No Hit AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 196 0.12278163036464891 No Hit AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 191 0.11964944591657113 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 186 0.11651726146849335 No Hit ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 178 0.1115057663515689 No Hit AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 167 0.10461496056579779 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.264368896155557E-4 0.0 12-13 0.0 0.0 0.0 6.264368896155557E-4 0.0 14-15 0.0 0.0 0.0 6.264368896155557E-4 0.0 16-17 0.0 0.0 0.0 9.396553344233335E-4 0.0 18-19 0.0 0.0 0.0 0.0012528737792311113 0.0 20-21 0.0 0.0 0.0 0.001879310668846667 0.0 22-23 0.0 0.0 0.0 0.005324713561732223 0.0 24-25 0.0 0.0 0.0 0.009083334899425557 0.0 26-27 0.0 0.0 0.0 0.016913796019620002 0.0 28-29 0.0 0.0 0.0 0.029442533811931116 0.0 30-31 0.0 0.0 0.0 0.05888506762386223 0.0 32-33 0.0 0.0 0.0 0.10837358190349114 0.0 34-35 0.0 0.0 0.0 0.1512845088421567 0.0 36-37 0.0 0.0 0.0 0.21768681914140559 0.0 38-39 0.0 0.0 0.0 0.3342040806098989 0.0 40-41 0.0 0.0 0.0 0.46982766721166674 0.0 42-43 0.0 0.0 0.0 0.5735029724430412 0.0 44-45 0.0 0.0 0.0 0.6928391999148046 0.0 46-47 0.0 0.0 0.0 0.8297156602958035 0.0 48-49 0.0 0.0 0.0 1.0044915524985436 0.0 50-51 0.0 0.0 0.0 1.2043249202859059 0.0 52-53 0.0 0.0 0.0 1.3881841473880714 0.0 54-55 0.0 0.0 0.0 1.5638996949252348 0.0 56-57 0.0 0.0 0.0 1.7671784656054825 0.0 58-59 0.0 0.0 0.0 1.9826727556332338 0.0 60-61 0.0 0.0 0.0 2.2507877443886914 0.0 62-63 0.0 0.0 0.0 2.5236010098162662 0.0 64-65 0.0 0.0 0.0 2.739095299844017 0.0 66-67 0.0 0.0 0.0 2.9796470654563905 0.0 68-69 0.0 0.0 0.0 3.2897333258160906 0.0 70-71 0.0 0.0 0.0 3.6565121246859986 0.0 72-73 0.0 0.0 0.0 4.023604142000714 0.0 74-75 0.0 0.0 0.0 4.333377183915607 0.0 76-77 0.0 0.0 0.0 4.662569769408581 0.0 78-79 0.0 0.0 0.0 5.058164665200804 0.0 80-81 0.0 0.0 0.0 5.509512444168813 0.0 82-83 0.0 0.0 0.0 5.961799878471243 0.0 84-85 0.0 0.0 0.0 6.372742478059048 0.0 86-87 0.0 0.0 0.0 6.766144844737617 0.0 88-89 0.0 0.0 0.0 7.188050089893693 0.0 90-91 0.0 0.0 0.0 7.700788684044026 0.0 92-93 0.0 0.0 0.0 8.252366365350522 0.0 94-95 0.0 0.0 0.0 8.780765881741244 0.0 96-97 0.0 0.0 0.0 9.259363665407529 0.0 98-99 0.0 0.0 0.0 9.766777545996128 0.0 100-101 0.0 0.0 0.0 10.37974604248495 0.0 102-103 0.0 0.0 0.0 11.027795004792242 0.0 104-105 0.0 0.0 0.0 11.66112270019357 0.0 106-107 0.0 0.0 0.0 12.22491590084757 0.0 108-109 0.0 0.0 0.0 12.79278094128407 0.0 110-111 0.0 0.0 0.0 13.44552818026348 0.0 112-113 0.0 0.0 0.0 14.159353015980404 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 85 0.0 77.260086 2 GAACAAA 705 0.0 71.979904 1 CATGATA 30 0.004173739 59.700974 2 TACAAGG 70 1.29675755E-8 59.70097 2 GTACAAG 185 0.0 51.633274 1 TAGGGAG 35 0.007738422 51.0758 5 AGAACAA 345 0.0 50.183434 2 GAGAACA 520 0.0 48.220016 1 GTCACTA 50 5.2309566E-4 47.76078 2 ACGCAAA 105 6.1390892E-9 45.486458 1 GGAAACA 225 0.0 45.107403 1 CGCAAAA 110 9.262294E-9 43.41889 2 ATATGGG 55 8.431196E-4 43.350666 3 TAACAAC 180 0.0 43.11737 1 GTATCAA 1100 0.0 42.33342 1 GTACATA 75 7.996322E-5 39.80065 1 GCAGAGA 325 0.0 38.5154 3 GTACATG 2085 0.0 38.368977 1 GAAAACA 530 0.0 38.29874 1 TACATGG 2080 0.0 37.600132 2 >>END_MODULE