##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940050_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 159633 Sequences flagged as poor quality 0 Sequence length 125 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.94207338081725 34.0 33.0 34.0 31.0 34.0 2 32.905451880250325 34.0 33.0 34.0 31.0 34.0 3 32.92473360771269 34.0 33.0 34.0 31.0 34.0 4 36.277799703068915 37.0 35.0 37.0 35.0 37.0 5 36.3860229401189 37.0 37.0 37.0 35.0 37.0 6 36.54418572600903 37.0 37.0 37.0 35.0 37.0 7 36.52220405555242 37.0 37.0 37.0 35.0 37.0 8 36.53661210401358 37.0 37.0 37.0 35.0 37.0 9 38.330789999561496 39.0 39.0 39.0 37.0 39.0 10-11 38.34667957126659 39.0 39.0 39.0 37.0 39.0 12-13 38.30273502346006 39.0 39.0 39.0 37.0 39.0 14-15 39.38782707836099 41.0 39.0 41.0 36.5 41.0 16-17 39.46803918989181 41.0 39.0 41.0 36.5 41.0 18-19 39.84152086348061 41.0 40.0 41.0 37.5 41.0 20-21 39.89846397674666 41.0 40.0 41.0 37.5 41.0 22-23 39.72200296931086 41.0 40.0 41.0 35.5 41.0 24-25 39.59610168323592 41.0 40.0 41.0 35.0 41.0 26-27 39.29858174688191 41.0 39.0 41.0 35.0 41.0 28-29 39.01681043393283 41.0 39.0 41.0 35.0 41.0 30-31 38.11222930095907 39.5 36.0 41.0 35.0 41.0 32-33 37.065268459529044 37.5 35.0 41.0 35.0 41.0 34-35 36.596465016631896 37.0 35.0 41.0 34.0 41.0 36-37 36.25285498612442 37.0 35.0 41.0 33.0 41.0 38-39 35.96506048248169 36.5 35.0 41.0 33.0 41.0 40-41 35.545307048041444 36.0 35.0 41.0 33.0 41.0 42-43 34.973912035731956 36.0 35.0 41.0 29.0 41.0 44-45 34.026448165479565 35.0 35.0 41.0 18.5 41.0 46-47 32.476624507463995 35.0 34.0 41.0 2.0 41.0 48-49 31.177763996166206 35.0 32.0 41.0 2.0 41.0 50-51 30.866800724161045 35.0 31.5 41.0 2.0 41.0 52-53 30.793576516133882 35.0 32.5 41.0 2.0 41.0 54-55 30.69006095230936 35.0 33.0 41.0 2.0 41.0 56-57 30.519601210276072 35.0 33.0 41.0 2.0 41.0 58-59 30.340972731202193 35.0 32.0 41.0 2.0 41.0 60-61 30.134680799082894 35.0 31.5 40.0 2.0 41.0 62-63 29.872570207914404 35.0 31.0 39.5 2.0 41.0 64-65 29.599944873553714 35.0 31.0 39.0 2.0 41.0 66-67 29.29271829759511 35.0 30.5 39.0 2.0 41.0 68-69 28.974817237037456 35.0 29.5 37.5 2.0 41.0 70-71 28.652023704371903 35.0 29.0 37.0 2.0 41.0 72-73 28.343381381042768 35.0 28.5 37.0 2.0 40.0 74-75 28.060134809218646 35.0 27.0 36.0 2.0 39.0 76-77 27.749249841824685 35.0 26.5 36.0 2.0 39.0 78-79 27.453806543759747 35.0 25.5 35.0 2.0 38.5 80-81 27.166685459773355 35.0 24.0 35.0 2.0 37.0 82-83 26.903522454630306 35.0 21.0 35.0 2.0 37.0 84-85 26.64394580068031 35.0 19.5 35.0 2.0 36.5 86-87 26.431088809957842 35.0 15.0 35.0 2.0 36.0 88-89 26.255836825718994 35.0 8.5 35.0 2.0 36.0 90-91 26.144017840922615 35.0 3.5 35.0 2.0 36.0 92-93 25.973232351706727 35.0 2.0 35.0 2.0 35.0 94-95 25.8201906873892 35.0 2.0 35.0 2.0 35.0 96-97 25.66558606303208 35.0 2.0 35.0 2.0 35.0 98-99 25.51525060607769 35.0 2.0 35.0 2.0 35.0 100-101 25.360476843760374 35.0 2.0 35.0 2.0 35.0 102-103 24.8524521872044 34.5 2.0 35.0 2.0 35.0 104-105 25.07870239862685 35.0 2.0 35.0 2.0 35.0 106-107 25.008153076118347 35.0 2.0 35.0 2.0 35.0 108-109 24.862644315398448 35.0 2.0 35.0 2.0 35.0 110-111 24.70958385797423 35.0 2.0 35.0 2.0 35.0 112-113 24.530673482300024 35.0 2.0 35.0 2.0 35.0 114-115 24.32991612010048 35.0 2.0 35.0 2.0 35.0 116-117 24.135147494565658 35.0 2.0 35.0 2.0 35.0 118-119 23.95571091190418 35.0 2.0 35.0 2.0 35.0 120-121 23.757932257114756 35.0 2.0 35.0 2.0 35.0 122-123 23.52291192923769 35.0 2.0 35.0 2.0 35.0 124-125 22.768152574968838 33.5 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31687645643302176 1101 2 0.22328698423894622 1101 3 0.3364669760504029 1101 4 0.06732638871889662 1101 5 0.05823464770131892 1101 6 0.1618811257739523 1101 7 0.07507996218622282 1101 8 0.1700036466650161 1101 9 0.3055408982926622 1101 10-11 0.28985608633177407 1101 12-13 0.2942696320917477 1101 14-15 0.3688214705601496 1101 16-17 0.399919639482718 1101 18-19 0.318508118713396 1101 20-21 0.25391792897763565 1101 22-23 0.07995271545954097 1101 24-25 0.2104454969596361 1101 26-27 0.050230137947444575 1101 28-29 -0.030602518245636645 1101 30-31 -0.015071278086026041 1101 32-33 0.3825689386544653 1101 34-35 -0.1385983798320325 1101 36-37 -0.29498066757239627 1101 38-39 0.03646728482254247 1101 40-41 -0.010146243944305411 1101 42-43 0.1032709178138802 1101 44-45 0.7950625878084168 1101 46-47 0.5980819111562212 1101 48-49 0.6988809439310941 1101 50-51 -0.5040886057208986 1101 52-53 -0.2039188588079739 1101 54-55 -0.1590494274338532 1101 56-57 -0.0389892411377879 1101 58-59 0.07260788822155462 1101 60-61 0.3344994268903214 1101 62-63 0.474270586394546 1101 64-65 0.10633871176715104 1101 66-67 -0.20486972103919499 1101 68-69 -0.39173564532041283 1101 70-71 -0.4278942478330272 1101 72-73 -0.1898571693927522 1101 74-75 -0.2953937161733471 1101 76-77 -0.21470987713105316 1101 78-79 -0.37127187912222936 1101 80-81 -0.32784051021581107 1101 82-83 -0.2783778530548844 1101 84-85 -0.30078353147758463 1101 86-87 -0.3739980231850524 1101 88-89 -0.7202123306821449 1101 90-91 -0.5726150411552986 1101 92-93 -0.5295096372193697 1101 94-95 -0.29727136303018753 1101 96-97 -0.14898244032276153 1101 98-99 -0.18719790022611704 1101 100-101 -0.172595811158903 1101 102-103 -0.13086550390534413 1101 104-105 -0.29097723344331783 1101 106-107 -0.3858549400265794 1101 108-109 -0.2578704203374258 1101 110-111 -0.3536910819584733 1101 112-113 -0.4879443767053857 1101 114-115 -0.6151809771466539 1101 116-117 -0.6540227917108048 1101 118-119 -1.0450939273339444 1101 120-121 -1.134854915306402 1101 122-123 -1.5022624783771938 1101 124-125 -1.78298426547655 1102 1 0.2997923021971616 1102 2 0.38056252603461616 1102 3 0.3355944739395156 1102 4 0.2705912353273945 1102 5 0.23645024015839766 1102 6 0.23761993804527037 1102 7 0.24547118087465236 1102 8 0.29387080118232234 1102 9 0.36447700862196086 1102 10-11 0.23369228109675788 1102 12-13 0.378888264233602 1102 14-15 0.4878898634675508 1102 16-17 0.4298520909322008 1102 18-19 0.20110029433962495 1102 20-21 0.16821570550451526 1102 22-23 0.26934196398191546 1102 24-25 0.18338385890728404 1102 26-27 0.2993717084512397 1102 28-29 0.30332655577631584 1102 30-31 0.1122858848748507 1102 32-33 0.5207197970839559 1102 34-35 0.6226315666920286 1102 36-37 0.763082940646008 1102 38-39 0.7185809915492527 1102 40-41 0.5495638601930395 1102 42-43 0.6661192150274999 1102 44-45 0.4179024414532222 1102 46-47 -0.20546438774611175 1102 48-49 -0.41956825392987085 1102 50-51 -0.3854986817130204 1102 52-53 -0.7796858163086746 1102 54-55 -0.46322899399732265 1102 56-57 -0.5581702149627255 1102 58-59 -0.8915632347730948 1102 60-61 -0.5860888084037938 1102 62-63 -0.4696922619026651 1102 64-65 -0.6312633146893631 1102 66-67 -0.48723673483036123 1102 68-69 -0.38011166558498033 1102 70-71 -0.15667274487778116 1102 72-73 0.024539115436972736 1102 74-75 -0.0065673722386456745 1102 76-77 -0.0020023060643517 1102 78-79 0.14178750731176848 1102 80-81 -0.40948982065769357 1102 82-83 -0.4783778530548872 1102 84-85 -0.834388935361627 1102 86-87 -0.49267238288108217 1102 88-89 -0.47626355499962614 1102 90-91 -0.30655818779194277 1102 92-93 -0.524837529141692 1102 94-95 -0.6447382923856608 1102 96-97 -0.7879354377896917 1102 98-99 -0.6203811256694038 1102 100-101 -0.6858944320426659 1102 102-103 -0.4882817718479302 1102 104-105 -0.2810278948562086 1102 106-107 -0.11937590822246946 1102 108-109 -0.33109023457890885 1102 110-111 -0.3930662578661561 1102 112-113 -0.16424609356437614 1102 114-115 -0.13064678069295255 1102 116-117 -0.2668288710803495 1102 118-119 -0.6618403388171998 1102 120-121 -0.6945368742143678 1102 122-123 -0.5822652929001322 1102 124-125 -0.47320379826573955 1103 1 0.19566352562885214 1103 2 0.12197787389964532 1103 3 0.19965158732478727 1103 4 0.040128953506496146 1103 5 -0.003159955558096783 1103 6 0.1768691033320593 1103 7 0.07839281284077515 1103 8 0.15453477143124417 1103 9 0.3069835913196428 1103 10-11 0.26873666340897984 1103 12-13 0.20160332164557815 1103 14-15 0.18716237357912036 1103 16-17 0.14580529270205744 1103 18-19 0.26637079571045774 1103 20-21 0.08587090787157337 1103 22-23 0.2188519941130309 1103 24-25 0.3338358255730469 1103 26-27 0.44341742087557634 1103 28-29 0.5335038135737662 1103 30-31 0.290833076709589 1103 32-33 0.33123311177762815 1103 34-35 0.7718077115385213 1103 36-37 0.7498496824142507 1103 38-39 0.7515487702620263 1103 40-41 1.2315449128817662 1103 42-43 1.6713446554574816 1103 44-45 1.8698555346425039 1103 46-47 0.9886776899432874 1103 48-49 0.9366982028143447 1103 50-51 1.2281716133547071 1103 52-53 1.4292096477375793 1103 54-55 1.2608677512997808 1103 56-57 1.2589936602781862 1103 58-59 1.4416434212164795 1103 60-61 1.5088649091238224 1103 62-63 1.6073456598650253 1103 64-65 1.3026518295870844 1103 66-67 1.4400007037537499 1103 68-69 1.1747351000709827 1103 70-71 1.147352880139188 1103 72-73 1.2016068968642628 1103 74-75 1.0479672242487723 1103 76-77 1.1931848597110495 1103 78-79 1.0067404104850333 1103 80-81 0.9875214988678103 1103 82-83 0.9708607256253146 1103 84-85 1.1442071177342754 1103 86-87 1.1858069460909277 1103 88-89 1.1464053556657028 1103 90-91 1.142666818315714 1103 92-93 1.1623418188319263 1103 94-95 0.7912806006353215 1103 96-97 0.7848406961987422 1103 98-99 0.6182789899689141 1103 100-101 0.3746256689372771 1103 102-103 0.49325952949032725 1103 104-105 0.5770029963189032 1103 106-107 0.15336493707735244 1103 108-109 0.23848277228881187 1103 110-111 0.23436662576260403 1103 112-113 0.35939732780970957 1103 114-115 0.5070605403526791 1103 116-117 0.5273023485510215 1103 118-119 0.35984863211034934 1103 120-121 0.6783831290430484 1103 122-123 0.9033613527742865 1103 124-125 1.0021212648467177 1104 1 0.30953914764380386 1104 2 0.3573808395669218 1104 3 0.23485950910718145 1104 4 0.05560743616509711 1104 5 0.04962692148924219 1104 6 0.11570534148564349 1104 7 0.09442141927722503 1104 8 0.19279337961808807 1104 9 0.22298969269245106 1104 10-11 0.14623410033228623 1104 12-13 -0.04022608997203747 1104 14-15 0.029814470300884466 1104 16-17 0.2755094761433341 1104 18-19 0.023628678734141317 1104 20-21 0.06154043351484262 1104 22-23 0.025921084658399707 1104 24-25 -0.0977862894028263 1104 26-27 -0.10603120506526409 1104 28-29 -0.13040806659927995 1104 30-31 0.1973995541670206 1104 32-33 0.31714244137678804 1104 34-35 0.2488400438132885 1104 36-37 0.21051583229797188 1104 38-39 0.07223342430918223 1104 40-41 -0.04570496834151072 1104 42-43 0.23580915997100504 1104 44-45 0.07975276151855581 1104 46-47 1.0117453801735863 1104 48-49 0.5764671508276464 1104 50-51 0.7885351951606161 1104 52-53 0.7816139387030461 1104 54-55 0.6389153120009397 1104 56-57 0.5071995065936079 1104 58-59 0.43122857787672686 1104 60-61 0.23425312147160327 1104 62-63 0.35731440283219484 1104 64-65 0.31095369750736523 1104 66-67 0.5889467166066424 1104 68-69 0.2720444946847671 1104 70-71 0.37450398913871297 1104 72-73 0.2433162814757992 1104 74-75 0.09436516378516657 1104 76-77 0.200574022272626 1104 78-79 0.45426610376602383 1104 80-81 0.4980863759651548 1104 82-83 0.7630014572899384 1104 84-85 0.6116873007754613 1104 86-87 0.5750945357986126 1104 88-89 0.9004125072748153 1104 90-91 0.82593305321857 1104 92-93 0.8032360200271924 1104 94-95 0.9928634567779575 1104 96-97 0.6437839584327492 1104 98-99 0.6684152706320603 1104 100-101 0.818913133616828 1104 102-103 0.5298552635304858 1104 104-105 0.8187323854314563 1104 106-107 0.7868699757110456 1104 108-109 1.1000891337201324 1104 110-111 1.1245044067633323 1104 112-113 0.9793618198204115 1104 114-115 1.2265224192754367 1104 116-117 1.4489847270861915 1104 118-119 1.481688546090993 1104 120-121 1.4564206874937007 1104 122-123 1.519062385506654 1104 124-125 1.4870053637689757 1105 1 -0.0821978625302151 1105 2 0.01609312018182152 1105 3 0.0675698588856335 1105 4 -0.00318405825280621 1105 5 0.013114309166539329 1105 6 0.10917020273771527 1105 7 0.21282130045651826 1105 8 0.10724247237249784 1105 9 0.22984663751508805 1105 10-11 0.13662942671791 1105 12-13 0.21652564901848592 1105 14-15 0.3974779106553612 1105 16-17 0.2527231306226341 1105 18-19 0.31532113114396765 1105 20-21 0.24928952379381997 1105 22-23 0.1113729746502301 1105 24-25 0.12090569267505202 1105 26-27 0.33531344924075057 1105 28-29 0.3998788358934817 1105 30-31 0.6108213429137095 1105 32-33 0.3649677749411637 1105 34-35 0.1727081528312766 1105 36-37 0.06897066805309748 1105 38-39 0.1059859306834241 1105 40-41 0.502429794140852 1105 42-43 0.49142220054331887 1105 44-45 0.9990165682368755 1105 46-47 0.46385816082561604 1105 48-49 0.21370506453411053 1105 50-51 -0.27056572817527424 1105 52-53 -0.5732391444466884 1105 54-55 -0.4397291417951372 1105 56-57 -0.58541875713885 1105 58-59 -0.6087991469543219 1105 60-61 -0.6004251962252525 1105 62-63 -0.4299346503153174 1105 64-65 -0.3645420076192565 1105 66-67 -0.3334732650751633 1105 68-69 -0.2335499801524712 1105 70-71 -0.25028250490787585 1105 72-73 -0.4098386557720133 1105 74-75 -0.5267081832455496 1105 76-77 -0.27666438652010683 1105 78-79 -0.47592673233566885 1105 80-81 -0.56499999183443 1105 82-83 -0.8452120239091592 1105 84-85 -0.6482577446487028 1105 86-87 -0.9640667880620697 1105 88-89 -1.2395855123801098 1105 90-91 -1.3502929041545073 1105 92-93 -1.1205702032072082 1105 94-95 -1.2147138385657605 1105 96-97 -0.8426878092727748 1105 98-99 -0.9258120234739344 1105 100-101 -0.9818393975117203 1105 102-103 -0.8167422560873057 1105 104-105 -1.2134977306662762 1105 106-107 -1.1948737181276066 1105 108-109 -1.5816075269229835 1105 110-111 -1.8406786513845503 1105 112-113 -1.8828928030476213 1105 114-115 -1.3328879329784442 1105 116-117 -1.4666649498700188 1105 118-119 -1.1983867070218608 1105 120-121 -1.2052992422040525 1105 122-123 -1.2340133379381584 1105 124-125 -1.0407070901261157 1106 1 -0.05387017759390744 1106 2 -0.06704349311723234 1106 3 -0.17503853190552832 1106 4 -0.26486455375356144 1106 5 -0.25828922010773425 1106 6 -0.08010173836313328 1106 7 -0.24829825078807488 1106 8 -0.14174665933254005 1106 9 -0.07512005641969921 1106 10-11 -0.06663106177104083 1106 12-13 0.0677702440868515 1106 14-15 0.013558312665409744 1106 16-17 0.18994411928688493 1106 18-19 -0.021210363538742172 1106 20-21 -0.1504884357714431 1106 22-23 -0.3136825354584474 1106 24-25 -0.039921700102794944 1106 26-27 -0.4012998498126521 1106 28-29 -0.4563675121256878 1106 30-31 -0.5435902499342546 1106 32-33 -0.845888833683361 1106 34-35 -1.2281332635529623 1106 36-37 -1.0522634093104557 1106 38-39 -1.2261398143206677 1106 40-41 -1.0351768436995172 1106 42-43 -0.6948930968740044 1106 44-45 -0.8332727855085977 1106 46-47 -0.33435382935785896 1106 48-49 -0.13018882351076755 1106 50-51 -0.0752636363206527 1106 52-53 -0.5563056397137274 1106 54-55 -1.019453344202521 1106 56-57 -0.740274430856271 1106 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-1.119426022337663 2216 42-43 -0.9283080295545361 2216 44-45 -0.36380167811956454 2216 46-47 -0.5888986982620636 2216 48-49 -0.9865761197808176 2216 50-51 -1.4003489935325355 2216 52-53 -0.7618135956500751 2216 54-55 -0.7719303138604481 2216 56-57 -0.7454989364286497 2216 58-59 -0.8204669040222115 2216 60-61 -0.7886307670154942 2216 62-63 -0.8146490811954763 2216 64-65 -0.9460158588699699 2216 66-67 -0.61691958253504 2216 68-69 -0.7945057284229087 2216 70-71 -0.749085383567099 2216 72-73 -1.1268377788110335 2216 74-75 -1.158551610910191 2216 76-77 -1.583823450538258 2216 78-79 -1.2056209095931472 2216 80-81 -1.3076189035676116 2216 82-83 -1.5781008447446396 2216 84-85 -1.7697586007296593 2216 86-87 -1.6372944220770194 2216 88-89 -1.8081624691862999 2216 90-91 -1.6083491131774608 2216 92-93 -1.5372658699063528 2216 94-95 -1.4949167923930666 2216 96-97 -1.751198506804755 2216 98-99 -1.835951362829995 2216 100-101 -1.6483575840120892 2216 102-103 -1.9017796313291662 2216 104-105 -1.7703401143297448 2216 106-107 -1.9035834718825306 2216 108-109 -2.073798398176759 2216 110-111 -2.238178616584513 2216 112-113 -2.135589806068264 2216 114-115 -2.4410812541549625 2216 116-117 -2.736017251544599 2216 118-119 -2.542600852541703 2216 120-121 -2.598428322508621 2216 122-123 -2.5943677415350876 2216 124-125 -2.7731504704626992 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 18.0 5 51.0 6 181.0 7 340.0 8 472.0 9 710.0 10 1243.0 11 1874.0 12 2234.0 13 3758.0 14 7415.0 15 7839.0 16 2850.0 17 1886.0 18 1661.0 19 1467.0 20 1372.0 21 1305.0 22 1311.0 23 1276.0 24 1327.0 25 1439.0 26 1602.0 27 1642.0 28 1774.0 29 1812.0 30 2106.0 31 2442.0 32 2848.0 33 3656.0 34 5394.0 35 22740.0 36 14509.0 37 34135.0 38 22739.0 39 201.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.79851139012104 16.405408114678092 15.959952885747938 12.836127609452925 2 17.384794647555566 22.541784649309644 42.588393013756985 17.4850276893778 3 17.90231343143335 34.4151898416994 33.57451153583532 14.10798519103193 4 9.665299261099193 20.807145634965437 38.12928882365486 31.398266280280506 5 7.7903602830123635 38.35080748766991 31.86207847388904 21.996753755428685 6 20.611600486172893 43.556956156728106 14.937912714423545 20.893530642675454 7 17.49888807452093 45.47305381719319 19.598704528512275 17.429353579773604 8 26.28278614071025 30.642160455544904 17.527077734553632 25.54797566919121 9 30.61334435862259 12.92715165410661 20.006514943652 36.4529890436188 10-11 23.18944077978864 29.98596781367261 21.8106531857448 25.013938220793946 12-13 30.76118346457186 30.243746593749414 25.995251608376712 12.999818333302013 14-15 25.506004397586967 24.636823213245382 35.7078423634211 14.149330025746556 16-17 13.47340462185137 29.34637574937513 34.98023591613263 22.19998371264087 18-19 12.822223475096001 17.628560510671353 53.777727662826614 15.771488351406038 20-21 14.717821503072672 15.611120507664456 57.28577424467372 12.38528374458915 22-23 13.89374377478341 16.501600546252966 57.57800705367938 12.026648625284245 24-25 13.044295352464715 16.433005706840063 58.01682609485508 12.505872845840146 26-27 12.782131514160605 16.42611490105429 58.092311740053745 12.699441844731352 28-29 12.984511299390789 16.287096925751335 57.89297292218063 12.835418852677243 30-31 13.153295371257823 16.371928110102548 57.930064585643315 12.54471193299631 32-33 12.630847005318449 16.628767234844926 57.64033752419613 13.100048235640498 34-35 12.933456532974175 16.377617339827417 57.776142076331574 12.912784050866835 36-37 13.380588296096295 16.255665427346276 56.769539655642255 13.594206620915175 38-39 13.36407117731998 16.41927087411788 56.50146119608218 13.71519675247996 40-41 13.404496563993659 16.58397699723741 55.95772803868875 14.053798400080183 42-43 13.840086950802007 16.75807568102587 54.84854084902321 14.553296519148908 44-45 14.19868321316035 17.111651245685362 53.53784665885699 15.151818882297299 46-47 14.720327250631135 18.052658284941085 50.918982917066025 16.30803154736176 48-49 15.303997018132387 19.053257032603213 48.30155702351979 17.341188925744603 50-51 15.631147027501097 19.972436258848585 46.76595877967801 17.63045793397231 52-53 15.55129578470617 20.74790300251201 45.52285554991762 18.177945662864197 54-55 15.56100555649521 20.84280819128877 45.30391585699699 18.292270395219035 56-57 16.145309666325254 20.456488850884693 44.823233509676975 18.574967973113075 58-59 16.115553634464376 20.07404553612539 45.148670531818595 18.661730297591642 60-61 16.634874695783726 20.095657811898025 45.63541656878498 17.634050923533263 62-63 17.437551876967408 19.802984981128528 45.91608851580975 16.84337462609431 64-65 17.41385628776275 20.029568099028076 46.1807350053091 16.37584060790007 66-67 17.8659466705924 20.197581289289953 45.62693219654139 16.30953984357626 68-69 18.159066847918012 20.165569344300295 45.31983136107648 16.355532446705215 70-71 17.697948086687152 20.613099544889323 45.042488481283456 16.64646388714007 72-73 17.674916840502902 20.84688003107127 44.56785251169871 16.91035061672712 74-75 17.02530178597157 21.116561112050768 44.81968014132416 17.0384569606535 76-77 16.257850307747727 21.488465333354213 44.97501996836385 17.278664390534214 78-79 15.990001941978688 21.282833534839725 45.12093515670515 17.606229366476438 80-81 15.72043374490237 21.222742164840604 44.899550844750145 18.15727324550688 82-83 15.841336064598172 21.04702661730344 44.03099609729818 19.08064122080021 84-85 16.003946564765947 20.9675348065087 43.70037429721391 19.32814433151144 86-87 15.883043866380591 20.816249823814072 43.35990478129453 19.9408015285108 88-89 15.636804420138692 20.823388647710686 43.55866268252805 19.98114424962257 90-91 15.76965843421609 21.047718979531112 43.44510046513085 19.737522121121952 92-93 15.523516588152752 21.189673749624134 43.45525959707327 19.831550065149845 94-95 15.394204777879054 21.173452608037888 43.51772676445439 19.914615849628674 96-97 15.855480556904139 21.31238939439024 43.172599564624996 19.659530484080623 98-99 15.98854873365783 20.969974879880727 43.150225830498705 19.89125055596274 100-101 16.04868667506092 21.35272781943583 43.08789535998196 19.510690145521288 102-103 16.150795888068256 21.306058271159472 42.94005625403269 19.603089586739582 104-105 16.239749926393664 21.339259426309095 42.84295853614228 19.57803211115496 106-107 15.895522855549919 21.658429021568224 42.54728032424374 19.898767798638126 108-109 16.02367938859568 21.586143172599563 42.25048157486727 20.139695863937483 110-111 15.89928147688761 21.903365845407905 42.02451873985955 20.17283393784493 112-113 15.682632554876216 22.113673950085197 41.69684023253483 20.506853262503757 114-115 15.393000106495606 22.398218391164622 41.3766749566187 20.832106545721068 116-117 15.242387889532388 22.13518093346823 41.67530641074231 20.947124766257076 118-119 15.107607207850581 22.589839724615757 41.48523317766231 20.81731988987136 120-121 15.07713028362019 22.810517908320673 41.096267990540774 21.016083817518364 122-123 15.274819585045604 22.927107346897866 40.362208078580736 21.435864989475796 124-125 14.94405883532124 23.193031472386487 39.962225618923526 21.900684073368748 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1093.0 1 819.5 2 1290.0 3 1853.0 4 985.5 5 578.5 6 1739.0 7 4525.0 8 8010.0 9 8552.5 10 5015.5 11 1795.0 12 1014.0 13 953.5 14 971.0 15 985.0 16 986.5 17 1041.5 18 1117.5 19 1100.5 20 1084.0 21 1125.5 22 1149.0 23 1253.5 24 1398.5 25 1483.0 26 1560.5 27 1700.0 28 1847.0 29 1978.0 30 2136.0 31 2275.5 32 2485.0 33 2694.0 34 2736.0 35 2710.0 36 2797.5 37 2990.0 38 3088.5 39 3044.0 40 2943.5 41 2885.5 42 2931.0 43 2907.0 44 3040.5 45 3321.0 46 3584.5 47 3913.0 48 4177.0 49 4389.0 50 4811.0 51 4471.0 52 3525.5 53 3189.5 54 3016.5 55 2808.0 56 2601.5 57 2432.5 58 2314.0 59 2156.5 60 1921.5 61 1733.5 62 1502.0 63 979.5 64 590.0 65 495.5 66 408.0 67 292.0 68 196.5 69 152.0 70 104.0 71 77.0 72 76.5 73 90.0 74 65.5 75 32.0 76 24.5 77 27.0 78 23.0 79 5.0 80 1.0 81 1.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013155174681926668 2 0.0031321844480777785 3 0.0 4 0.12967243615042 5 0.04009196093539556 6 0.011902300902695558 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.1321844480777783E-4 30-31 0.0 32-33 0.0 34-35 3.1321844480777783E-4 36-37 0.0015660922240388893 38-39 0.0021925291136544447 40-41 0.0 42-43 0.0015660922240388893 44-45 0.0012528737792311113 46-47 0.0 48-49 9.396553344233335E-4 50-51 0.001879310668846667 52-53 0.0 54-55 0.0 56-57 9.396553344233335E-4 58-59 9.396553344233335E-4 60-61 9.396553344233335E-4 62-63 3.1321844480777783E-4 64-65 9.396553344233335E-4 66-67 0.0015660922240388893 68-69 0.0012528737792311113 70-71 9.396553344233335E-4 72-73 0.0 74-75 0.0 76-77 0.003445402892885556 78-79 0.0012528737792311113 80-81 0.0 82-83 0.0 84-85 3.1321844480777783E-4 86-87 3.1321844480777783E-4 88-89 0.0 90-91 3.1321844480777783E-4 92-93 6.264368896155557E-4 94-95 9.396553344233335E-4 96-97 3.1321844480777783E-4 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 3.1321844480777783E-4 110-111 0.0 112-113 6.264368896155557E-4 114-115 0.0012528737792311113 116-117 0.0015660922240388893 118-119 9.396553344233335E-4 120-121 3.1321844480777783E-4 122-123 6.264368896155557E-4 124-125 6.264368896155557E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 159633.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.43462817838417 #Duplication Level Percentage of deduplicated Percentage of total 1 77.32058382369908 33.58390808917955 2 12.246163609091958 10.638151259451368 3 4.4219453097957775 5.761966510683881 4 2.1345332871812626 3.7085063865240895 5 1.179762316833968 2.5621268785276228 6 0.6562247605861313 1.7101727086504668 7 0.4384446752047998 1.3330577011019025 8 0.2668166608976578 0.9271265966310224 9 0.21345332871812622 0.8344139369679201 >10 0.9720779970001154 7.636892121303239 >50 0.0764393677166263 2.2708337248563892 >100 0.05913234106380524 5.228868717621044 >500 0.0028845044421368406 0.9835059166964224 >1k 0.007211261105342103 5.7005756955015565 >5k 0.0028845044421368406 9.469846460318356 >10k+ 0.0014422522210684203 7.650047295985166 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12212 7.650047295985166 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8975 5.622271084299612 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6142 3.8475753760187428 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2246 1.406977254076538 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2134 1.336816322439596 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1981 1.2409714783284158 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1667 1.0442702949891314 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1072 0.6715403456678757 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 866 0.5424943464070712 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 704 0.4410115702893512 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 395 0.2474425713981445 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 387 0.24243107628122004 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 363 0.2273965909304467 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 354 0.2217586589239067 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 322 0.20171267845620894 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 322 0.20171267845620894 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 286 0.17916095043004893 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 285 0.17853451354043337 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 264 0.1653793388585067 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 256 0.16036784374158225 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 249 0.15598278551427336 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 243 0.15222416417658002 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 237 0.1484655428388867 No Hit GCGTTGATACCACTGCTTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 230 0.1440804846115778 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 223 0.1396954263842689 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 223 0.1396954263842689 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 214 0.1340574943777289 No Hit GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 212 0.1328046205984978 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 208 0.13029887304003557 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 195 0.12215519347503336 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 194 0.1215287565854178 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 180 0.11275864013080002 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 174 0.10900001879310668 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 173 0.10837358190349113 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 166 0.10398852367618223 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.264368896155557E-4 0.0 12-13 0.0 0.0 0.0 6.264368896155557E-4 0.0 14-15 0.0 0.0 0.0 6.264368896155557E-4 0.0 16-17 0.0 0.0 0.0 9.396553344233335E-4 0.0 18-19 0.0 0.0 0.0 0.0012528737792311113 0.0 20-21 0.0 0.0 0.0 0.001879310668846667 0.0 22-23 0.0 0.0 0.0 0.005324713561732223 0.0 24-25 0.0 0.0 0.0 0.009083334899425557 0.0 26-27 0.0 0.0 0.0 0.016913796019620002 0.0 28-29 0.0 0.0 0.0 0.029442533811931116 0.0 30-31 0.0 0.0 0.0 0.05888506762386223 0.0 32-33 0.0 0.0 0.0 0.10837358190349114 0.0 34-35 0.0 0.0 0.0 0.15222416417658002 0.0 36-37 0.0 0.0 0.0 0.21862647447582895 0.0 38-39 0.0 0.0 0.0 0.3363966097235534 0.0 40-41 0.0 0.0 0.0 0.4707673225460901 0.0 42-43 0.0 0.0 0.0 0.5750690646670802 0.0 44-45 0.0 0.0 0.0 0.6962846028076901 0.0 46-47 0.0 0.0 0.0 0.8381725583056134 0.0 48-49 0.0 0.0 0.0 1.0154541980668157 0.0 50-51 0.0 0.0 0.0 1.2152875658541782 0.0 52-53 0.0 0.0 0.0 1.4013393220699981 0.0 54-55 0.0 0.0 0.0 1.577681306496777 0.0 56-57 0.0 0.0 0.0 1.785031916959526 0.0 58-59 0.0 0.0 0.0 2.006164138993817 0.0 60-61 0.0 0.0 0.0 2.278350967531776 0.0 62-63 0.0 0.0 0.0 2.554922854297044 0.0 64-65 0.0 0.0 0.0 2.7738625472176803 0.0 66-67 0.0 0.0 0.0 3.018799371057363 0.0 68-69 0.0 0.0 0.0 3.328572412972255 0.0 70-71 0.0 0.0 0.0 3.698170177845433 0.0 72-73 0.0 0.0 0.0 4.069960471832266 0.0 74-75 0.0 0.0 0.0 4.388816848646583 0.0 76-77 0.0 0.0 0.0 4.722081273922059 0.0 78-79 0.0 0.0 0.0 5.121748009496783 0.0 80-81 0.0 0.0 0.0 5.5790469389161395 0.0 82-83 0.0 0.0 0.0 6.040417708117996 0.0 84-85 0.0 0.0 0.0 6.458877550381187 0.0 86-87 0.0 0.0 0.0 6.862302907293604 0.0 88-89 0.0 0.0 0.0 7.298929419355647 0.0 90-91 0.0 0.0 0.0 7.827328935746368 0.0 92-93 0.0 0.0 0.0 8.396760068406909 0.0 94-95 0.0 0.0 0.0 8.944265909930904 0.0 96-97 0.0 0.0 0.0 9.440717144951233 0.0 98-99 0.0 0.0 0.0 9.97663390401734 0.0 100-101 0.0 0.0 0.0 10.607455851860205 0.0 102-103 0.0 0.0 0.0 11.28338125575539 0.0 104-105 0.0 0.0 0.0 11.949596887861532 0.0 106-107 0.0 0.0 0.0 12.543459059217078 0.0 108-109 0.0 0.0 0.0 13.134502264569356 0.0 110-111 0.0 0.0 0.0 13.817005255805505 0.0 112-113 0.0 0.0 0.0 14.564344465116863 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 55 0.0 108.34527 3 TATAACG 55 0.0 108.34527 2 TATCACG 30 3.5321318E-7 99.3165 2 GTATAAC 70 0.0 85.15514 1 ATCACGC 35 8.8430716E-7 85.128426 3 GGTATCA 2275 0.0 81.74894 1 TAACGCA 80 0.0 74.487366 4 TCACGCA 45 3.9376937E-6 66.211 4 CACGCAG 50 7.4114923E-6 59.5152 5 GTCTGCT 30 0.004230849 59.496555 8 CGACCGA 15 0.0042347233 59.496555 114-115 ATCCCGC 15 0.0042347233 59.496555 76-77 GTATCAA 4460 0.0 58.806694 1 TTAGATA 35 0.0077376897 51.077053 4 GTAGACA 35 0.0077376897 51.077053 3 ATAAGTA 35 0.0077376897 51.077053 4 TAGATAG 35 0.00777617 51.013027 5 AGTAGAC 50 5.2792596E-4 47.671917 2 CCGTGCA 25 5.3283025E-4 47.597244 16-17 GTGGTAT 655 0.0 47.322857 1 >>END_MODULE